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Molecular Biology Reports

, Volume 36, Issue 4, pp 669–676 | Cite as

Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA)

  • Xinya Chen
  • Xiuting Gu
  • Yuxi Shan
  • Wenwen Tang
  • Jian Yuan
  • Zhaomin Zhong
  • Yingli Wang
  • Weixue Huang
  • Bo Wan
  • Long Yu
Article

Abstract

l-lactate dehydrogenase is a crucial enzyme in the process of glycolysis. Here we report the cloning and characterization of another novel lactate dehydrogenase gene, named as LDHAL6A (lactate dehydrogenase A-like 6A), which encodes a 332-amino-acid protein. The LDHAL6A gene consists of seven exons, and is mapped to 11p15.1 by searching the UCSC genomic database. By RT-PCR analysis in various tissues, LDHAL6A was found to be exclusively expressed in human testis. Subcellular localization demonstrated that LDHAL6A protein was located in the cytoplasm when overexpressed in COS7 cells. Furthermore, we found that the recombinant protein GST-LDHAL6A can catalyze the pyruvate convert into the lactate with NADH as its coenzyme. And in the dual luciferase reporter system, expression of LDHAL6A was able to activate transcriptional activities of AP1(PMA).

Keywords

LDHAL6A Lactate dehydrogenase Catalysis Transcription AP1(PMA) 

Abbreviations

LDH

l-lactate dehydrogenase

LDHAL6A

Lactate dehydrogenase A-like 6A

NAD+

Nicotinamide adenine dinucleotide

Notes

Acknowledgements

This work was supported by the National 973 program of China, 863 projects of China and the National Natural Science foundation of China (30024001).

Supplementary material

11033_2008_9227_MOESM1_ESM.tif (1.2 mb)
LDHAL6A was located in the cytoplasm and might be on mitochondria. (ac) COS7 cells were transiently transfected with pCMV-Myc-LDHAL6A. (a) Cells expressing Myc-tagged LDHAL6A were stained with monoclonal antibody against Myc epitope (green). (b) The cells transfected with pCMV-Myc-LDHAL6A was stained using mitotracker (red) as cell mitochondria localization marker. (c) The nuclei of the cells was stained with DAPI. (d) The superimposed image of (a), (b) and (c) beach pollutant (TIF 1267 kb)

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Xinya Chen
    • 1
  • Xiuting Gu
    • 1
  • Yuxi Shan
    • 1
  • Wenwen Tang
    • 1
  • Jian Yuan
    • 1
  • Zhaomin Zhong
    • 1
  • Yingli Wang
    • 1
  • Weixue Huang
    • 1
  • Bo Wan
    • 1
  • Long Yu
    • 1
  1. 1.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life ScienceFudan UniversityShanghaiP.R. China

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