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Molecular Biology Reports

, Volume 36, Issue 2, pp 263–272 | Cite as

Primary molecular features of self-incompatible and self-compatible F1 seedling from apricot (Prunus armeniaca L.) Katy × Xinshiji

  • J. R. Feng
  • X. S. Chen
  • Z. H. Yuan
  • L. J. Zhang
  • Z. J. Ci
  • X. L. Liu
  • C. Y. Zhang
Article

Abstract

Expression of the S-RNase genes in the self-compatible (SC) apricot (Prunus armeniaca L.) cultivar Katy, the self-incompatible (SI) cultivar Xinshiji and their F1 seedling was examined in this study. Three S-genotypes, S9Sc (Sc, self-compatibility S-gene absent from the style), S8S9, and S8S10, were obtained. Seedlings with S-RNase that migrated as a single band in gel electrophoresis were SC, despite high transcript abundance, and those with S-RNase that migrated as two bands were SI with high transcript abundance or SC with low transcript expression. S8-RNase was induced in SI cultivars only 24 h after self-pollination, indicating post-transcriptional regulation of S8-RNase in SI apricots. A Proteomic study showed that 35 protein spots were synthesized differently between SC and SI pistils. Fifteen of the 35 protein spots were identified; nine proteins, including receptor protein kinase-like protein, reversibly glycosylated polypeptide-2, and isoflavone reductase-like protein, were detected only in the SC pistils; while nine proteins, including actin 7, a putative serine/threonine kinase, and S-RNase, were detected only in the SI pistils. A mitochondrial NAD-dependent malate dehydrogenase and a probable elongation factor G were up-regulated, while heat shock cognate 70 was down-regulated in the SC pistils compared to those in the SI pistils. The results suggest that the proteins responsible for self-compatibility and self-incompatibility may be different.

Keywords

Apricot F1 Seedling Self-incompatibility S-RNase gene Proteome 

Abbreviations

ACN

Acetonitrile

BB

Bromophenol blue

BS

At balloon stage

DTT

Dithiothreitol

FQRT-PCR

Fluorescence quantification RT-PCR technology

HPLC

High-pressure liquid chromatography

IAM

Iodoacetamide

IEF

Isoelectric focusing electrophoresis

LC-ESI-MS/MS

Liquid chromatography-electrospray ion trap tandem mass spectrometry

MS

Mass spectrometer

PMSF

Phenylmethyl sulfonyl fluoride

RT-PCR

Reverse transcript-PCR

SI

Self-incompatible

SC

Self-compatible

TFA

Triflouroacetic acid

2-DE

Two-dimensional gel electrophoresis

Notes

Acknowledgements

The research was supported by the National Natural Science Foundation (No. 30370992 and 30760143), the Special Program for Doctorial Site of Universities (No. 200404344011) and the Key Project for Excellent College Teachers from Ministry of Education, China (No. 204178), which are gratefully acknowledged. The authors sincerely thank Dr. Guanggan Hu in Michael Smith Laboratories, the University of British Columbia, Canada for his carefully reading of this manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • J. R. Feng
    • 1
  • X. S. Chen
    • 2
  • Z. H. Yuan
    • 2
  • L. J. Zhang
    • 2
  • Z. J. Ci
    • 2
  • X. L. Liu
    • 2
  • C. Y. Zhang
    • 2
  1. 1.Horticultural DepartmentShihezi UniversityShiheziChina
  2. 2.Horticultural Science and Engineering CollegeShandong Agricultural UniversityTaianChina

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