Abstract
A full-length cDNA encoding 10-deacetylbaccatin III-10-O-acetyl transferase (designated as TmDBAT), which catalyzes the acetylation of the C-10 hydroxyl group of the advanced metabolite 10-deacetylbaccatin III (10-DAB) to yield baccatin III, the immediate diterpenoid precursor of Taxol, was isolated from Taxus x media. Heterologous expression of TmDBAT in E. coli demonstrated that TmDBAT was a functional gene. Tissue expression pattern analysis revealed that TmDBAT expressed strongly in leaves, weak in stems and no expression could be detected in fruits, implying that TmDBAT was tissue-specific. Expression profiling analysis of TmDBAT under different elicitor treatments including silver nitrate, ammonium ceric sulphate and methyl jasmonate indicated that TmDBAT was an elicitor-responsive gene. Southern blot analysis suggested that TmDBAT belonged to a small multigene family.
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This work was funded by China National “863” High-tech Program, China Ministry of Education, and Shanghai Natural Science Fundation (05ZR14093).
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Binhui Guo and Guoyin Kai contributed equally to this work.
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Guo, B., Kai, G., Gong, Y. et al. Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media . Mol Biol Rep 34, 89–95 (2007). https://doi.org/10.1007/s11033-006-9018-6
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DOI: https://doi.org/10.1007/s11033-006-9018-6