Molecular Biology Reports

, Volume 34, Issue 1, pp 11–17 | Cite as

Group I intron renders differential susceptibility of Candida albicans to Bleomycin

  • Prathiba Jayaguru
  • Malathi Raghunathan
Original Paper


The alarming increase in drug resistance gained by fungal pathogens has raised an urgent need to develop drugs against novel targets. Candida albicans, an opportunistic fungal pathogen, harbors in its 25S rRNA gene, a self-splicing Group I intron, which can act as a selective drug target. We report that Bleomycin selectively inhibits the self-splicing of Group I intron of C. albicans at IC50 = 1.2 μM, leading to accumulation of precursor RNA as evinced by Reverse Transcriptase PCR. Drug susceptibility assays including MIC determination, growth curve analysis and disc diffusion assays indicate a strong susceptibility of the intron-containing strain (4-1) than the intronless strain (62-1). These results on the preferential targeting of Group I intron of C. albicans by Bleomycin might form a basis for design of small molecules that inhibit self-splicing of RNA as a antimicrobial tool against life-threatening microorganisms.


Group I intron Bleomycin Candida albicans 



We sincerely thank Dr. G. Jayaraman, Dept. of Genetics, University of Madras for his valuable suggestions and for providing gel documentation facilities.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Genetics, Dr ALM Post Graduate Institute of Basic Medical SciencesUniversity of MadrasChennaiIndia

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