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Molecular Biology Reports

, Volume 33, Issue 3, pp 215–221 | Cite as

Detection and transcript expression of S-RNase gene associated with self-incompatibility in apricot (Prunus armeniaca L.)

  • Jianrong Feng
  • Xuesen Chen
  • Yan Wu
  • Wen Liu
  • Qing Liang
  • Lijie Zhang
Research Article

Abstract

The identity and expression of S-RNase genotypes in the self-compatible (SC) apricot cultivar ‘Katy’ and the self-incompatible (SI) cultivar ‘Xinshiji’ were examined. We used allele specific polymerase chain reaction (AS-PCR) and designated the alleles in ‘Katy’ and ‘Xinshiji’ as S8Sc and S9S10, respectively. The S-RNase gene was expressed in style at the balloon stage in both genotypes. Using real-time fluorescence quantification RT-PCR technology (FQRT-PCR), spatio-temporal expression patterns of S-RNase gene between ‘Katy’ and ‘Xinshiji’ were compared. The results revealed that the expression of the S-RNase gene in ‘Katy’ and ‘Xinshiji’ were different. The transcript abundance was distinctly diverse at the key stage (i.e., at 24 h after self-pollination) in both genotypes, and was greater in ‘Xinshiji’ (SI) than ‘Katy’ (SC). In addition, the abundance of the S-RNase transcript was higher in upper-half of style than in the lower-half of style or in the ovary. In the SI cultivar ‘Xinshiji’, the expression of S-RNase reminded a relatively high level after cross-pollination, but it dropped continuously after self-pollination and un-pollination.

Keywords

Apricot Self-incompatibility S-RNase gene FQRT-PCR 

Abbreviations

ACP

After cross-pollination

AS-PCR

Allele specific polymerase chain reaction

ASP

After self-pollination

BA

Before anthesis

BS

At balloon stage

FQRT-PCR

Fluorescence quantification RT-PCR technology

RT-PCR

Reverse transcript-PCR

SI

Self-incompatible

SC

Self-compatible

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Jianrong Feng
    • 1
    • 2
  • Xuesen Chen
    • 1
  • Yan Wu
    • 1
  • Wen Liu
    • 1
  • Qing Liang
    • 1
  • Lijie Zhang
    • 1
  1. 1.Horticultural Science and Engineering CollegeShandong Agricultural UniversityTaian, ShandongChina
  2. 2.Horticultural DepartmentShihezi UniversityShihezi, XinjiangChina

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