Molecular Biology Reports

, Volume 33, Issue 1, pp 59–63 | Cite as

Semi-anaerobic Growth Conditions are Favoured by some Escherichia coli Strains During Heterologous Expression of Some Archaeal Proteins



Host cell physiology is known to play a crucial role in the expression of foreign genes in heterologous systems. Expression of archaeal genes in anaerobic or semi-anaerobic growth conditions of E. coli has been previously reported to be a means of improving solubility of some proteins. Here, we report that some of the Rosetta strains of E. coli, which harbour the rare tRNA genes for the expression of archaeal genes, favour semi-anaerobic conditions for the expression of putative FMN binding domain of glutamate synthase from Methanocaldococcus jannaschii at low inducer concentrations.


glutamate synthase Methanocaldococcus jannaschii putative FMN binding domain Rosetta strains semi-anaerobic expression 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Vanoni, MA, Curti, B 2005Arch. Biochem. Biophys.433193211CrossRefPubMedGoogle Scholar
  2. 2.
    Suzuki, A, Knaff, DB 2005Photosynth. Res.83191217CrossRefPubMedGoogle Scholar
  3. 3.
    Hirel B & Lea PJ (2003) In: Foyer CH & Noctor G (Eds.) Photosynthetic Nitrogen Assimilation and Associated Carbon and Respiratory Metabolism (pp. 69–92) Kluwer Academic Publishers, Dordrecht.Google Scholar
  4. 4.
    Vanoni, MA, Edmondson, DE, Zanetti, G, Curti, GB 1992Biochemistry3146134623CrossRefPubMedGoogle Scholar
  5. 5.
    Navarro, F, Chávez, S, Candau, S, Florencio, FJ 1995Plant. Mol. Biol.27753767CrossRefPubMedGoogle Scholar
  6. 6.
    Binda, C, Bossi, RT, Wakatsuki, S, Arzt, S, Coda, A, Curti, B, Vanoni, MA, Mattevi, A 2000Structure812991308CrossRefPubMedGoogle Scholar
  7. 7.
    Heuvel, RH, Ferrari, D, Bossi, RT, Ravasio, S, Curti, B, Vanoni, MA, Florencio, FJ, Mattevi, A 2002J. Biol. Chem.2772457924583PubMedGoogle Scholar
  8. 8.
    Ravasio, S, Dossena, L, Figuerosa, EM, Florencio, FJ, Mattevi, A, Morandi, P, Curti, B, Vanoni, MA 2002Biochemistry4181208133CrossRefPubMedGoogle Scholar
  9. 9.
    Bult, CJ, White, O, Olsen, GJ, Zhou, L, Fleischmann, RG, Sutton, GG, Blake, JA, FitzGerald, GA, Clayton, RA, Gocayne, JD, Kerlavage, AR, Dougherty, BA, Tomb, JB, Adams, MD, Reich, CI, Overbeek, R, Kirkness, EF, Weinstock, KG, Merrick, JM, Glodek, A, Scott, JL, Geoghagen, NSM, Weidman, JF, Fuhrmann, JL, Nguyen, D, Utterback, TR, Kelley, JM, Peterson, JD, Sadow, PW, Hanna, MC, Cotton, MD, Roberts, KM, Hurst, MA, Kaine, BP, Borodovsky, M, Klenk, HP, Fraser, CM, Smith, HO, Woese, CR, Venter, JC 1996Science27310581073PubMedGoogle Scholar
  10. 10.
    Dincturk, HB, Knaff, DB 2000Mol. Biol. Rep.27141148CrossRefPubMedGoogle Scholar
  11. 11.
    Schut, GJ, Brehm, SD, Datta, S, Adams, MWW 2003J. Bacteriol.18539353947CrossRefPubMedGoogle Scholar
  12. 12.
    Jongsareejit, B, Rahman, RNZA, Fujiwara, S, Imanaka, T 1997Mol. Gen. Genet.254635642PubMedGoogle Scholar
  13. 13.
    Sambrook, F, Fritsch, EF, Maniatis, T 1989Molecular CloningCold Spring Harbour Laboratory PressNew YorkGoogle Scholar
  14. 14.
    Laemmli, UK 1970Nature22680685Google Scholar
  15. 15.
    Bradford, MM 1976Anal. Biochem.72248254CrossRefPubMedGoogle Scholar
  16. 16.
    Kashima, Y, Ishikawa, K 2003J. Biochem.1342529CrossRefPubMedGoogle Scholar
  17. 17.
    Kim, R, Sandler, SJ, Goldman, S, Yokota, A, Clark, AJ, Kim, S-H 1998Biotech. Lett.20207210Google Scholar
  18. 18.
    Jannasch, HW, Mottl, MJ 1986Science229717725Google Scholar
  19. 19.
    Broderick, JB, Henshaw, TF, Cheek, J, Wojtuszewski, K, Smith, SR, Trojan, MR, McGhan, RM, Kopf, A, Kibbey, M, Broderick, WE 2000Biochem. Biophys. Res. Commun.269451456CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Molecular Biology and Genetics Faculty of Sciences and LettersIstanbul Technical UniversityMaslakTurkey

Personalised recommendations