Molecular Biology Reports

, Volume 33, Issue 2, pp 97–102 | Cite as

Identification of Epitope on DNA-binding Protein Expressed in Insect Cell Infected by Baculovirus

  • Mami Suzuki
  • Tetsuya Kohsaka
  • Enoch Y. Park


DNA-binding protein (DBP) is an early gene product produced during viral replication. Polyclonal anti-DBP was produced using rabbit by intradermal injections of Escherichia coli-expressed purified recombinant DBP. Prepared anti-DBP completely blocked the replication of baculovirus in insect cells. The anti-DBP binding to DBP was confirmed by both Western blotting with Tn-5B1-4 insect cell lysates as well as immunostained baculovirus-infected Tn-5B1-4 insect cells. To determine the anti-DBP epitope 12 peptides were synthesized and their specific-binding activities were measured using ELISA. Based on specific-binding activity against anti-DBP the epitope was predicted to be between amino acid residues 248–265 (QRMSVEDFDRLFEMDKID). Especially from 18 amino acid residues it was further to be narrowed between amino acid residues 260–265 (EMDKID) which showed a critical role in specific-binding activity.


anti-DBP baculovirus DNA-binding protein (DBP) epitope insect cell 


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Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Laboratory of BiotechnologyShizuoka UniversityShizuokaJapan
  2. 2.Laboratory of Animal Reproduction and Physiology, Department of Applied Biological Chemistry, Faculty of AgricultureShizuoka UniversityShizuokaJapan

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