Molecular Breeding

, 38:71 | Cite as

Proteomic and functional analysis of soybean chlorophyll-deficient mutant cd1 and the underlying gene encoding the CHLI subunit of Mg-chelatase

  • Hongyang Du
  • Mengzhe Qi
  • Xiaopei Cui
  • Yanmei Cui
  • Hui Yang
  • Jinyu Zhang
  • Yujie Ma
  • Shanshan Zhang
  • Xi Zhang
  • Deyue Yu
Article
  • 36 Downloads

Abstract

Sufficient chlorophyll biosynthesis is vital for the growth of photoautotrophic plants. Mg-chelatase, consisting of three subunits (CHLI, CHLD, and CHLH), catalyzes the insertion of Mg2+ into protoporphyrin IX, which is the rate-limiting step of chlorophyll biosynthesis. A chlorophyll-deficient mutant (cd1) was previously identified by phenotype. This mutant shows a chlorina phenotype, abnormal chloroplasts, and lower chlorophyll content, plant height, and seed yield than the WT (Nannong 86-4). In this study, map-based cloning of cd1 revealed that a missense mutation (G1709A) was present in GmCHLI1b, leading to an amino acid substitution (D278N) in the Arg-finger domain. Yeast two-hybrid assays indicated that this substitution weakened the interaction between Gmcd1 and GmCHLI1a/b. In addition, GmCHLI1b was not a target protein of GmTrxF1/2 or GmNTRC1/2 in the Y2H system but could interact with them in a bimolecular fluorescence complementation (BiFC) assay. A proteomic analysis of leaf soluble proteins identified 31 differentially accumulated proteins, mainly involved in photosynthesis, carbon fixation in photosynthetic organisms, and carbohydrate/energy and amino acid/protein metabolism. These results indicated that cd1 possesses weaker photosynthesis, respiration and protein metabolism and that its leaf redox homeostasis is disrupted. Global transcriptome analysis of chlorophyll biosynthesis and RT-PCR demonstrated a transcriptional downregulation of photosynthesis-associated genes in cd1 during the diurnal rhythm. This study provides insights into the mechanisms of molecular regulation in the cd1 mutant and adds to the knowledge of the biological and biochemical functions of GmCHLIs in soybean.

Keywords

Soybean Map-based cloning Mg-chelatase GmCHLI1b Proteomes 2-DE Thioredoxin 

Notes

Funding information

This work was supported in part by the Ministry of Science and Technology (2016YFD0100304, 2017YFE0111000), Key Transgenic Breeding Program of China (2016ZX08004-003), National Natural Science Foundation of China (31701453,31671715), Fundamental Research Funds for the Central Universities (Y0201600116), and Jiangsu Collaborative Innovation Center for Modern Crop Production (JCIC-MCP).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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11032_2018_819_MOESM2_ESM.xlsx (37 kb)
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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Hongyang Du
    • 1
    • 2
  • Mengzhe Qi
    • 1
  • Xiaopei Cui
    • 1
  • Yanmei Cui
    • 1
  • Hui Yang
    • 1
  • Jinyu Zhang
    • 3
  • Yujie Ma
    • 1
  • Shanshan Zhang
    • 1
  • Xi Zhang
    • 1
  • Deyue Yu
    • 1
    • 4
  1. 1.National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.Provincial Key Laboratory of Agrobiology, Institute of BiotechnologyJiangsu Academy of Agricultural SciencesNanjingChina
  3. 3.Henan Collaborative Innovation Center of Modern Biological BreedingHenan Institute of Science and TechnologyXinxiangChina
  4. 4.School of Life SciencesGuangzhou UniversityGuangzhouChina

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