Molecular Breeding

, 38:53 | Cite as

Identification and mapping of net form of net blotch resistance in South African barley

  • Anke Martin
  • Gregory J. Platz
  • Daniel de Klerk
  • Ryan A. Fowler
  • Francois Smit
  • Francois G. Potgieter
  • Renée Prins


Net form of net blotch (NFNB) caused by the fungus Pyrenophora teres f. teres is an economically important foliar disease of barley (Hordeum vulgare) in southern and eastern Africa. Little attention has been given to disease resistance breeding, and knowledge about the presence of NFNB resistance in breeding lines is limited. Deploying resistance into varieties used in this region is important for future control of the disease. We have identified NFNB disease resistance in existing South African breeders’ lines and have mapped the resistance in line UVC8. Six different trials, three conducted in South Africa and another three in Australia, were used to identify resistance QTL. A major QTL was identified on chromosome 6H having a LOD score of 40.5 and 55% of the phenotypic variance explained. Kompetitive Allele Specific PCR (KASP™) markers were designed for this QTL region. These and microsatellite markers can now be used to routinely select for NFNB resistance.


Hordeum vulgare Net form of net blotch Quantitative trait loci KASP™ markers 



We would like to thank the South African Winter Cereal Trust and the South African National Research Foundation for funding this project and Judy McIlroy, Janet Barsby, Lizaan Rademeyer, and Debbie Snyman for their technical support. We would also like to thank Dr. Kelvin Khoo for his help with the initial KAPS™ marker development.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

Supplementary material

11032_2018_814_MOESM1_ESM.docx (13 kb)
Supplementary Table 1 SABBI lines and standards (last five listed lines) screened for adult NFNB resistance in 2008 in Australia. Lines in bold were also screened in 2009 in Australia and those underlined were screened in South Africa in 2011 and 2012. The pedigree of each line is given (DOCX 12 kb)
11032_2018_814_MOESM2_ESM.docx (12 kb)
Supplementary Table 2 Summary of linkage map of DH population UVC8/Erica. The number of markers located on each chromosome is indicated together with the linkage group sizes and the average distance between markers (DOCX 11 kb)
11032_2018_814_MOESM3_ESM.xlsx (582 kb)
Supplementary Table 3 Genotype data of UVC8/Erica population (XLSX 581 kb)
11032_2018_814_MOESM4_ESM.xlsx (8 kb)
Supplementary Table 4 List of SSR markers added to the UVC8/Erica genetic map (XLSX 8 kb)
11032_2018_814_MOESM5_ESM.docx (54 kb)
Supplementary Fig. 1 Distribution of infection response scores of the UVC8/Erica population across different environments and years. Parental scores are indicated (DOCX 54 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centre for Crop HealthUniversity of Southern QueenslandToowoombaAustralia
  2. 2.Queensland Department of Agriculture and Fisheries, Hermitage Research FacilityWarwickAustralia
  3. 3.South African Barley Breeding InstituteCaledonSouth Africa
  4. 4.CenGen (Pty) LtdWorcesterSouth Africa
  5. 5.Department of Plant SciencesUniversity of the Free StateBloemfonteinSouth Africa

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