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Molecular Breeding

, 38:23 | Cite as

Molecular mapping of powdery mildew resistance gene PmSGD in Chinese wheat landrace Shangeda using RNA-seq with bulk segregant analysis

  • Xiaodan Xu
  • Qiang Li
  • Zhanhong Ma
  • Jieru Fan
  • Yilin Zhou
Article

Abstract

Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of the most devastating diseases of wheat in China and causes serious yield losses. Resistance genes are urgently needed by wheat breeding programs to combat this disease. In the present study, genetic analysis of powdery mildew resistance was conducted on segregated F2 and F2:3 populations derived from the cross of Shangeda (providing good resistance to powdery mildew) and Chancellor (susceptible to powdery mildew). The results showed that the resistance of Shangeda to E09 was controlled by a single recessive gene, tentatively designated as PmSGD. In addition, RNA sequencing of the parental lines Shangeda and Chancellor and the corresponding bulked pools derived from homozygous resistant or susceptible F2:3 lines was implemented to identify single-nucleotide polymorphisms (SNPs). The PmSGD gene was estimated to be located in the 240–250-Mb region of chromosome 7B based on the characteristics of putative SNP loci distributed on 21 wheat chromosomes. Among the developed SNP markers, 17 (57%) markers were linked to PmSGD flanked by SNP2-57 and SNP2-46, with genetic distances of 0.4 and 0.8 cM, respectively. The reaction patterns of Shangeda and cultivars (lines) carrying the Pm5e, Pmhym, mlxbd, and PmTm4 genes to 22 Bgt isolates indicated that PmSGD may be allelic or very closely linked to those genes. All of the SNP loci linked to PmSGD were used to test 38 cultivars with known Pm gene(s), and the results suggested that these SNP loci are useful for pyramiding PmSGD by marker-assisted selection.

Keywords

Wheat landrace Powdery mildew Genetic mapping RNA-seq 

Notes

Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2016YFD0300705), the Special Fund for Agro-scientific Research in the Public Interest (201303016). The authors are grateful to Dr. S.C. Xu and T.G. Liu, Chinese Academy of Agricultural Sciences, China, and Dr. X.M. Xu, NIAB East Malling Research, Kent, UK, for the excellent proposal for this research.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Xiaodan Xu
    • 1
    • 2
  • Qiang Li
    • 3
  • Zhanhong Ma
    • 4
  • Jieru Fan
    • 1
  • Yilin Zhou
    • 1
  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Department of PathophysiologyHarbin Medical University—DaqingDaqingChina
  3. 3.College of Plant ProtectionNorthwest A & F University/State Key Laboratory of Crop Stress Biology for Arid AreasYanglingChina
  4. 4.College of Plant ProtectionChina Agricultural UniversityBeijingChina

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