Molecular Breeding

, 38:23 | Cite as

Molecular mapping of powdery mildew resistance gene PmSGD in Chinese wheat landrace Shangeda using RNA-seq with bulk segregant analysis

  • Xiaodan Xu
  • Qiang Li
  • Zhanhong Ma
  • Jieru Fan
  • Yilin Zhou


Wheat powdery mildew, caused by the fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of the most devastating diseases of wheat in China and causes serious yield losses. Resistance genes are urgently needed by wheat breeding programs to combat this disease. In the present study, genetic analysis of powdery mildew resistance was conducted on segregated F2 and F2:3 populations derived from the cross of Shangeda (providing good resistance to powdery mildew) and Chancellor (susceptible to powdery mildew). The results showed that the resistance of Shangeda to E09 was controlled by a single recessive gene, tentatively designated as PmSGD. In addition, RNA sequencing of the parental lines Shangeda and Chancellor and the corresponding bulked pools derived from homozygous resistant or susceptible F2:3 lines was implemented to identify single-nucleotide polymorphisms (SNPs). The PmSGD gene was estimated to be located in the 240–250-Mb region of chromosome 7B based on the characteristics of putative SNP loci distributed on 21 wheat chromosomes. Among the developed SNP markers, 17 (57%) markers were linked to PmSGD flanked by SNP2-57 and SNP2-46, with genetic distances of 0.4 and 0.8 cM, respectively. The reaction patterns of Shangeda and cultivars (lines) carrying the Pm5e, Pmhym, mlxbd, and PmTm4 genes to 22 Bgt isolates indicated that PmSGD may be allelic or very closely linked to those genes. All of the SNP loci linked to PmSGD were used to test 38 cultivars with known Pm gene(s), and the results suggested that these SNP loci are useful for pyramiding PmSGD by marker-assisted selection.


Wheat landrace Powdery mildew Genetic mapping RNA-seq 



This work was financially supported by the National Key Research and Development Program of China (2016YFD0300705), the Special Fund for Agro-scientific Research in the Public Interest (201303016). The authors are grateful to Dr. S.C. Xu and T.G. Liu, Chinese Academy of Agricultural Sciences, China, and Dr. X.M. Xu, NIAB East Malling Research, Kent, UK, for the excellent proposal for this research.

Supplementary material

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  1. Bennett FGA (1984) Resistance to powdery mildew in wheat: a review of its use in agriculture and breeding programmes. Plant Pathol 33(3):279–300. CrossRefGoogle Scholar
  2. Cao SQ, Luo HS, Wu CP, Jin SL, Jin MA, Jia QZ, Zhang B, Huang J, Wang XM (2010) Evaluation of 193 Gansu landraces on wheat to powdery mildew. Gansu Agricutural Science and Technology 5:8–10Google Scholar
  3. Chen Z, Wang B, Dong X, Liu H, Ren L, Chen J, Hauck A, Song W, Lai J (2014) An ultra-high density bin-map for rapid QTL mapping for tassel and ear architecture in a large F2 maize population. BMC Genomics 15:1–10CrossRefGoogle Scholar
  4. Coram TE, Settles ML, Chen X (2008) Transcriptome analysis of high-temperature adult-plant resistance conditioned by Yr39 during the wheat-Puccinia striiformis f. sp. tritici interaction. Mol Plant Pathol 9(4):479–493. CrossRefPubMedGoogle Scholar
  5. Fu B, Chen Y, Kong Z, Zhang L, Jia H, Ma Z (2013) pmX: a recessive powdery mildew resistance gene at the Pm4 locus identified in wheat landrace Xiaohongpi. Theor Appl Genet 126(4):913–921. CrossRefPubMedGoogle Scholar
  6. Guo Y, Yuan H, Fang D, Song L, Liu Y, Liu Y, Wu L, Yu J, Li Z, Xu X, Zhang H (2014) An improved 2b-RAD approach (I2b-RAD) offering genotyping tested by a rice (Oryza sativa L.) F2 population. BMC Genomics 15(1):956–956. CrossRefPubMedPubMedCentralGoogle Scholar
  7. Hansey CN, Vaillancourt B, Sekhon RS, de Leon N, Kaeppler SM, Buell CR (2012) Maize (Zea mays L.) genome diversity as revealed by RNA-sequencing. PLoS One 7(3):e33071. CrossRefPubMedPubMedCentralGoogle Scholar
  8. Herrera-Foessel SA, Singh RP, Huerta-Espino J, Rosewarne GM, Periyannan SK, Viccars L, Calvo-Salazar V, Lan C, Lagudah ES (2012) Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat. Theor Appl Genet 124(8):1475–1486. CrossRefPubMedGoogle Scholar
  9. Hsam SLK, Huang XQ, Zeller FJ (2001) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.) 6. Alleles at the Pm5 locus. Theor Appl Genet 102(1):127–133. CrossRefGoogle Scholar
  10. Hu TZ, Li HJ, Xie CJ, You MS, Yang ZM, Sun QX, Liu ZY (2008) Molecular mapping and chromosomal location of powdery mildew resistance gene in wheat variety Tangmai 4. Acta Agron Sin 34:1193–1198Google Scholar
  11. Hu XY, Ohm HW, Dweikat I (1997) Identification of RAPD markers linked to the gene PM1 for resistance to powdery mildew in wheat. Theor Appl Genet 94(6-7):832–840. CrossRefGoogle Scholar
  12. Huang XQ, Röder MS (2011) High-density genetic and physical bin mapping of wheat chromosome 1D reveals that the powdery mildew resistance gene Pm24 is located in a highly recombinogenic region. Genetica 139(9):1179–1187. CrossRefPubMedGoogle Scholar
  13. Huang XQ, Wang LX, Xu MX, Röder M (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.) Theor Appl Genet 106(5):858–865. CrossRefPubMedGoogle Scholar
  14. Huo ZG, Ye CL, Qian S, Chen L, Liu WC (2002) Relationship between climatic anomaly and prevailling of the wheat powdery mildew in China. Journal of Natural Disasters 11:85–90Google Scholar
  15. Kosambi DD (1943) The estimation of map distances from recombination values. Ann Hum Genet 12:172–175Google Scholar
  16. Lan C, Zhang Y, Herrera-Foesse SA, Basnet BR, Huerta-Espino J, Lagudah ES, Singh RP (2015) Identification and characterization of pleiotropic and co-located resistance loci to leaf rust and stripe rust in bread wheat cultivar Sujata. Theor Appl Genet 128(3):549–561. CrossRefPubMedGoogle Scholar
  17. Law CN, Wolfe MS (1966) Location of genetic factors for mildew resistance and ear emergence time on chromosome 7B of wheat. Can J Genet Cytol 8(3):462–470. CrossRefGoogle Scholar
  18. Li Y, Niu YC, Chen XM (2009) Mapping a stripe rust resistance gene YrC591 in wheat variety C591 with SSR and AFLP markers. Theor Appl Genet 118(2):339–346. CrossRefPubMedGoogle Scholar
  19. Li ZF, Zheng TC, He ZH, Li GQ, Xu SC, Li XP, Yang GY, Singh RP, Xia XC (2006) Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 112(6):1098–1103. CrossRefPubMedGoogle Scholar
  20. Liu J, Liu D, Tao W, Li W, Wang S, Chen P, Cheng S, Gao D (2000) Molecular marker-facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breed 119(1):21–24. CrossRefGoogle Scholar
  21. Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas (Beijing) 25:317–321Google Scholar
  22. Lowry JR, Sammons DJ, Baenziger PS, Moseman JG (1984) Identification and characterization of the gene conditioning powdery mildew resistance in ‘Amigo’ wheat. Crop Sci 24(1):129–132. CrossRefGoogle Scholar
  23. Luo PG, Luo HY, Chang ZJ, Zhang HY, Zhang M, Ren ZL (2009) Characterization and chromosomal location of Pm40 in common wheat: a new gene for resistance to powdery mildew derived from Elytrigia intermedium. Theor Appl Genet 118(6):1059–1064. CrossRefPubMedGoogle Scholar
  24. Maccaferri M, Mantovani P, Tuberosa R, Deambrogio E, Giuliani S, Demontis A, Massi A, Sanguineti MC (2008) A major QTL for durable leaf rust resistance widely exploited in durum wheat breeding programs maps on the distal region of chromosome arm 7BL. Theor Appl Genet 117(8):1225–1240. CrossRefPubMedGoogle Scholar
  25. Petersen S, Lyerly JH, Worthington ML, Parks WR, Cowger C, Marshall DS, Brown-Guedira G, Murphy JP (2015) Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat. Theor Appl Genet 128(2):303–312. CrossRefPubMedGoogle Scholar
  26. Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ, Sheng BQ, Xiang QJ, Duan XY, Zhou YL (1995) New resistance resource to powdery mildew—gene Pm21. Acta Agron Sin 21:257–262Google Scholar
  27. Ramirez-Gonzalez RH, Segovia V, Bird N, Fenwick P, Holdgate S, Berry S, Jack P, Caccamo M, Uauy C (2015) RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat. Plant Biotechnology J 13(5):613–624. CrossRefGoogle Scholar
  28. Ren RS, Wang MN, Chen XM, Zhang ZJ (2012) Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI183527. Theor Appl Genet 125(5):847–857. CrossRefPubMedGoogle Scholar
  29. Ren Y, Li SR, Wei YM, Zhou Q, Du XY, He YJ, Zheng YL (2015) Molecular mapping of a recessive stripe rust resistance gene yrMY37 in Chinese wheat cultivar Mianmai 37. Mol Breeding 35:293–293Google Scholar
  30. Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5(2):69–76. CrossRefPubMedGoogle Scholar
  31. Sheng BQ (1988) Wheat powdery mildew was recorded using infection type in seedling stage. Plant Prot 1:49Google Scholar
  32. Takagi H, Abe A, Yoshida K, Kosugi S, Natsume S, Mitsuoka C, Uemura A, Utsushi H, Tamiru M, Takuno S, Innan H, Cano LM, Kamoun S, Terauchi R (2013) QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant J 74(1):174–183. CrossRefPubMedGoogle Scholar
  33. Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, van Baren MJ, Salzberg SL, Wold BJ, Pachter L (2010) Transcript assembly and quantification by RNA-seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol 28(5):511–515. CrossRefPubMedPubMedCentralGoogle Scholar
  34. Trick M, Adamski NM, Mugford SG, Jiang CC, Febrer M, Uauy C (2012) Combining SNP discovery from next-generation sequencing data with bulked segregant analysis (BSA) to fine-map genes in polyploid wheat. BMC Plant Biol 12(1):14. CrossRefPubMedPubMedCentralGoogle Scholar
  35. Wan YX, Zhang XK, Xia XC, Zhang PZ, He ZH (2008) Development of multiplex PCR and identification of major quality genes in cultivars from Yellow and Huai River valley wheat region. Sci Agric Sin 41:643–653Google Scholar
  36. Wang JM, Kang ZS, Liu HY (2009) Genetic analysis and location of a powdery mildew resistance gene in wheat landrace Hongyoumai by SSR marker. Acta Phytopathologica Sinica 39:285–289Google Scholar
  37. Wang XY, Chen PD, Zhang SZ (2001) Pyramiding and marker-assisted selection for powdery mildew resistance genes in common wheat. Acta Genet Sin 28(7):640–646PubMedGoogle Scholar
  38. Wang Z, Li H, Zhang D, Zhang D, Guo L, Chen J, Chen Y, Wu Q, Xie J, Zhang Y, Sun Q, Dvorak J, Luo MC, Liu Z (2015) Genetic and physical mapping of powdery mildew resistance gene MlHLT in Chinese wheat landrace Hulutou. Theor Appl Genet 128(2):365–373. CrossRefPubMedGoogle Scholar
  39. Worthington M, Lyerly J, Petersen S, Brown-Guedira G, Marshall D, Cowger C, Parks Ryan MJP (2014) MlUM15: an Aegilops neglecta-derived powdery mildew resistance gene in common wheat. Crop Sci 54(4):1397–1406. CrossRefGoogle Scholar
  40. Xiao M, Song F, Jiao J, Wang X, Xu H, Li H (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126(5):1397–1403. CrossRefPubMedGoogle Scholar
  41. Xu X, Bai G, Carver BF, Shaner GE, Hunger RM (2005) Molecular characterization of slow leaf-rusting resistance in wheat. Crop Sci 45(2):758–765. CrossRefGoogle Scholar
  42. Xue F, Zhai WW, Duan XY, Zhou YL, Ji WQ (2009) Microsatellite mapping of powdery mildew resistance gene in wheat land-race Xiaobaidong. Acta Agron Sin 35:1806–1811Google Scholar
  43. Yuan XP (2009) Identification and evaluation on main agronomic characters and disease resistance of the spring wheat landraces. Journal of Anhui Agricultural Sciences 37:28–30Google Scholar
  44. Zhai WW, Duan XY, Zhou YL, Ma H (2008) Inheritance of resistance to powdery mildew in four Chinese landraces. Plant Prot 34:37–40Google Scholar
  45. Zhang H, Yang Y, Wang C, Liu M, Li H, Fu Y, Wang Y, Nie Y, Liu X, Ji W (2014) Large-scale transcriptome comparison reveals distinct gene activations in wheat responding to stripe rust and powdery mildew. BMC Genomics 15(1):898. CrossRefPubMedPubMedCentralGoogle Scholar
  46. Zhang R, Sun B, Chen J, Cao A, Xing L, Feng Y, Lan C, Chen P (2016) Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. Theor Appl Genet 129(10):1975–1984. CrossRefPubMedGoogle Scholar
  47. Zhang Y, Wang L, Xin H, Li D, Ma C, Ding X, Hong W, Zhang X (2013) Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment (SLAF) sequencing. BMC Plant Biol 13(1):141. CrossRefPubMedPubMedCentralGoogle Scholar
  48. Zhao NJ, Xue F, Wang CY, Han JR, Ji WQ, Zheng L (2010) SSR analysis of powdery mildew resistance gene in Chinese wheat landrace Baihulu. Journal of Triticeae Crops 30:411–414Google Scholar
  49. Zhao X, Xu H, Li X, An D (2012) Genetic analysis of resistance to powdery mildew in three Chinese wheat landraces. Plant Prot 38:51–54Google Scholar
  50. Zhou R, Zhu Z, Kong X, Huo N, Tian Q, Li P, Jin C, Dong Y, Jia J (2005) Development of wheat near-isogenic lines for powdery mildew resistance. Theor Appl Genet 110(4):640–648. CrossRefPubMedGoogle Scholar
  51. Zhou XL, Wang MN, Chen XM, Lu Y, Kang ZS, Jing JX (2014) Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759. Theor Appl Genet 127(4):935–945. CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Xiaodan Xu
    • 1
    • 2
  • Qiang Li
    • 3
  • Zhanhong Ma
    • 4
  • Jieru Fan
    • 1
  • Yilin Zhou
    • 1
  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Department of PathophysiologyHarbin Medical University—DaqingDaqingChina
  3. 3.College of Plant ProtectionNorthwest A & F University/State Key Laboratory of Crop Stress Biology for Arid AreasYanglingChina
  4. 4.College of Plant ProtectionChina Agricultural UniversityBeijingChina

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