Skip to main content
Log in

Allele-specific CAPS marker in a Ve1 homolog of Capsicum annuum for improved selection of Verticillium dahliae resistance

  • Short Communication
  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Verticillium wilt (Verticillium dahliae) is an economically important disease for many high-value crops. The pathogen is difficult to manage due to the long viability of its resting structures, wide host range, and the inability of fungicides to affect the pathogen once in the plant vascular system. In chile pepper (Capsicum annuum), breeding for resistance to Verticillium wilt is especially challenging due to the limited resistance sources. The dominant Ve locus in tomato (Solanum lycopersicum) contains two closely linked and inversely oriented genes, Ve1 and Ve2. Homologs of Ve1 have been characterized in diverse plant species, and interfamily transfer of Ve1 confers race-specific resistance. Queries in the chile pepper WGS database in NCBI with Ve1 and Ve2 sequences identified one open reading frame (ORF) with homology to the tomato Ve genes. Comparison of the candidate CaVe (Capsicum annuum Ve) gene sequences from susceptible and resistant accessions revealed 16 single nucleotide polymorphisms (SNPs) and several haplotypes. A homozygous haplotype was identified for the susceptible accessions and for resistant accessions. We developed a cleaved amplified polymorphic sequence (CAPS) molecular marker within the coding region of CaVe and screened diverse germplasm that has been previously reported as being resistant to Verticillium wilt in other regions. Based on our phenotyping using the New Mexico V. dahliae isolate, the marker could select resistance accessions with 48% accuracy. This molecular marker is a promising tool towards marker-assisted selection for Verticillium wilt resistance and has the potential to improve the efficacy of chile pepper breeding programs, but does not eliminate the need for a bioassay. Furthermore, this work provides a basis for future research in this important pathosystem.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Bae J, Halterman D, Jansky S (2008) Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids. Mol Breed 22:61–69

    Article  CAS  Google Scholar 

  • Chai Y, Zhao L, Liao Z, Sun X, Zuo K, Zhang L, Wang S, Tang K (2003) Molecular cloning of a potential Verticillium dahliae resistance gene SlVe 1 with multi-site polyadenylation from Solanum lycopersicoides. DNA Seq 14:375–384

    Article  CAS  PubMed  Google Scholar 

  • Chen T, Kan J, Yang Y, Ling X, Chang Y, Zhang B (2016) A Ve homologous gene from Gossypium barbadense, Gbvdr3, enhances the defense response against Verticillium dahliae. Plant Physiol Biochem 98:101–111

    Article  CAS  PubMed  Google Scholar 

  • Fei J, Chai Y, Wang J, Lin J, Sun X, Sun C, Zuo K, Tang K (2004) cDNA cloning and characterization of the Ve homologue gene StVe from Solanum torvum Swartz. DNA Seq 15:88–95

    Article  CAS  PubMed  Google Scholar 

  • Fradin EF, Zhang Z, Ayala JCJ, Castroverde CDM, Nazar RN, Robb J, Liu CM, Thomma BPHJ (2009) Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1. Plant Physiol 150:320–332

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gurung S, Short DPG, Hu X, Sandoya GV, Hayes RJ, Subbarao KV (2015) Screening of wild and cultivated Capsicum germplasm reveals new sources of verticillium wilt resistance. Plant Dis 99:1404–1409

    Article  Google Scholar 

  • Hayes RJ, McHale LK, Vallad GE, Truco MJ, Michelmore RW, Klosterman SJ, Maruthachalam K, Subbarao KV (2011) The inheritance of resistance to Verticillium wilt cause by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theor Appl Genet 123:509–517

    Article  CAS  PubMed  Google Scholar 

  • Kawchuk LM, Hachey J, Lynch DR, Kulcsar F, van Rooijen G, Waterer DR, Robertson A, Kokko E, Byers R, Howard RJ, Fischer R, Prüfer D (2001) Tomato Ve disease resistance genes encode cell surface-like receptors. Proc Natl Acad Sci U S A 98:6511–6515

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu J, Zheng Z, Zhou X, Feng C, Zhuang Y (2015) Improving the resistance of eggplant (Solanum melongena) to Verticillium wilt using wild species Solanum linnaeanum. Euphytica 201:463–469

    Article  CAS  Google Scholar 

  • Park YH, Lee YJ, Kang JS, Choi YW, Son BG (2008) Development of gene-based DNA marker for verticillium wilt resistance in tomato. Kor J Hort Sci Technol 26:313–319

    CAS  Google Scholar 

  • Song Y, Zhang Z, Seidl MF, Majer A, Jakse J, Javornik B, Thomma BPHJ (2017) Broad taxonomic characterization of the Verticillium wilt resistance gene reveals an ancient origin of the tomato Ve1 immune receptor. Mol Plant Pathol 18:195–209

    Article  CAS  PubMed  Google Scholar 

  • Tang J, Lin J, Yang Y, Chen t LX, Zhang B, Chang Y (2016) Ectopic expression of a Ve homolog VvVe gene from Vitis vinifera enhances defense response to Verticillium dahlia infection in tobacco. Gene 576:492–498

    Article  CAS  PubMed  Google Scholar 

  • Uribe P, Jansky S, Halterman D (2014) Two CAPS markers predict Verticillium wilt resistance in wild Solanum species. Mol Breed 33:465–476

    Article  CAS  Google Scholar 

  • Vining K, Davis T (2009) Isolation of a Ve homolog, mVe1, and its relationship to verticillium wilt resistance in Mentha longifolia (L.) Huds. Mol Gen Genomics 282:173–184

    Article  CAS  Google Scholar 

  • Wang G, Ellendorff U, Kemp B, Mansfield JW, Forsyth A, Mitchell K, Bastas K, Liu CM, Woods-Tor A, Zipfel C, de Wit PJ, Jones JDG, Tor M, Thomma BPHJ (2008) A genome-wide function investigation into the roles of receptor-like proteins in Arabidopsis. Plant Physiol 147:503–517

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang G, Fiers M, Ellendorff U, Wang Z, de Wit PJ, Angenent GC, Thomma BPHJ (2010) The diverse roles of extracellular leucine-rich repeat containing receptor-like proteins in plants. Crit Rev Plant Sci 29:174–182

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Paul W. Bosland.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Barchenger, D.W., Rodriguez, K., Jiang, L. et al. Allele-specific CAPS marker in a Ve1 homolog of Capsicum annuum for improved selection of Verticillium dahliae resistance. Mol Breeding 37, 134 (2017). https://doi.org/10.1007/s11032-017-0735-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11032-017-0735-4

Keywords

Navigation