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Molecular Breeding

, 36:128 | Cite as

Genome re-sequencing of two accessions and fine mapping the locus of lobed leaflet margins in mungbean

  • Keyuan Jiao
  • Xin Li
  • Wuxiu Guo
  • Xingxing Yuan
  • Xiaoyan Cui
  • Xin Chen
Article

Abstract

Mungbean (Vigna radiata L.) is a fast-growing warm-season legume crop widely distributed in Asia. A large amount of self-shading of mungbean plants could reduce its seed yield. Thus, modification of the leaflet type could affect the leaf canopy and improve yield. In this study, a classical locus, lobed leaflet margins (lma) in mungbean, was investigated, which was controlled by a semi-dominant allele. In order to map the lma, the whole genomes of AL127 carrying lma and another accession Sulu with oval leaflets were re-sequenced; 236,998 single nucleotide polymorphisms and 8896 insertion/deletions (InDels) were identified between the two accessions. Using the validated InDels and SNP markers, lma was mapped to a syntenic region about 376 kb on chromosome 3 in mungbean and on chromosome 1 in common bean, respectively. Our results provided a framework for map-based gene cloning in mungbean. Cloning the lma should shed light on the underlying molecular mechanism controlling leaf shape in legumes and further provide the molecular basis for genetic improvement on legume crops.

Keywords

Mungbean lma Genome re-sequencing SNP InDel Synteny 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 31271786) and the Ministry of Agriculture of China for Transgenic Research (grant no. 2014ZX0800943B).

Supplementary material

11032_2016_552_MOESM1_ESM.xlsx (9 kb)
Table S1 The number of SNPs and InDels detected on the mungbean chromosomes and scaffold between AL127 and VC1973A, Sulu and VC1973A. (XLSX 8 kb)
11032_2016_552_MOESM2_ESM.xlsx (6.6 mb)
Table S2 The SNPs between AL127 and Sulu. (XLSX 6726 kb)
11032_2016_552_MOESM3_ESM.xlsx (1.3 mb)
Table S3 The InDels between AL127 and Sulu. (XLSX 1280 kb)
11032_2016_552_MOESM4_ESM.xlsx (11 kb)
Table S4 Developing new markers for fine mapping of the lma locus in mungbean. (XLSX 11 kb)
11032_2016_552_MOESM5_ESM.xlsx (24 kb)
Table S5 The SNP between AL127 and Sulu in the lma region. (XLSX 23 kb)
11032_2016_552_MOESM6_ESM.xlsx (9 kb)
Table S6 The InDels between AL127 and Sulu in the lma region. (XLSX 9 kb)
11032_2016_552_MOESM7_ESM.xlsx (15 kb)
Table S7 The homolog genes in the syntenic block. (XLSX 14 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Keyuan Jiao
    • 1
    • 2
  • Xin Li
    • 2
  • Wuxiu Guo
    • 1
  • Xingxing Yuan
    • 2
    • 3
  • Xiaoyan Cui
    • 3
  • Xin Chen
    • 3
  1. 1.Guangdong Key Laboratory of Plant Resources, School of Life SciencesSun Yat-Sen UniversityGuangzhouChina
  2. 2.College of Life Sciences, Laboratory Center of Life SciencesNanjing Agricultural UniversityNanjingChina
  3. 3.Institute of Vegetable CropsJiangsu Academy of Agricultural SciencesNanjingChina

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