QTL analysis and the development of closely linked markers for days to flowering in spring oilseed rape (Brassica napus L.)
- 472 Downloads
Days to flowering (DTF) is an important trait impacting cultivar performance in oilseed rape (Brassica napus L.), but the interaction of all loci controlling this trait in spring-type oilseed rape is not fully understood. We identified quantitative trait loci (QTL) for variation in DTF in a doubled haploid (DH) population from the Qinghai–Tibet Plateau that includes 217 lines derived from a cross between spring-type oilseed rape (B. napus L.) line No. 5246 and line No. 4512, the latter of which is responsive to the effective accumulated temperature (EAT). A linkage map was constructed for the DH population, using 202 SSR and 293 AFLP markers. At least 22 DTF QTL were found in multiple environments. Four major QTL were located on linkage groups A7, C2, C8 and C8. Among these QTL, cqDTFA7a and cqDTFC2a were identified in five environments and individually explained 10.4 and 23.0 % of the trait variation, respectively. cqDTFC8, a major QTL observed in spring environments, and a unique winter environment QTL, qDTFC8-3, were identified; these QTL explained 10.0 and 46.5 % of the phenotypic variation, respectively. Minor QTL (for example, cqDTFC2c) and epistatic interactions seemed evident in this population. Two closely linked SSR markers for cqDTFA7a and cqDTFC8 were developed (G1803 and S034). BnAP1, a B. napus gene with homology to Arabidopsis thaliana that was identified as a cqDTFA7a candidate gene, played a major role in this study. The allelic effects of the major and minor QTL on DTF were further validated in the DH population and in 93 breeding genotypes.
KeywordsSpring Brassica napus L. Doubled haploid lines Days to flowering QTL analysis Molecular markers
The authors thank Prof. Ruisheng Wang, Mr. Jianshe Qin and Mr. Jianrong Lin for providing help with field management. The authors also thank Dr. Jun Zou (HAU China) for assistance with data analysis. Financial support for this study was provided by the National System of Technology of the Rapeseed Industry (CARS-13), the “973” Program Early Research Project (2012CB723007), the “863” High-tech Program (2011AA10A104) and the National Science and Technology Support Program (2010BD01B03).
Compliance with ethical standards
Conflict of interest
We declare that we do not have any commercial or associative interests that represent a conflict of interest in connection with the work submitted.
This article does not describe any studies involving human participants or animals.
- Du DZ, Liu QY, Li XP, Yu QL, Fu Z, Wang RS, Zhao HC, Tang GY, An FY, Wang NH (2004) Breeding for a hybrid variety Qingza No.3 with very early maturity and double-low quality in Brassica napus L. Chin J Oil Crop Sci 26(1):66–68Google Scholar
- Du DZ, Nie P, Xu L, Luo YX, Yao YM, Zhou HW, Zhang XM (2010) Rapeseed heterosis of different ecotypes in Qinghai province. Chin J Oil Crop Sci 32(2):180–186Google Scholar
- Kim SY, Park BS, Kwon SJ, Kim J, Lim MH, Park YD, Kim DY, Suh SC, Jin YM, Ahn JH, Lee YH (2007) Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese cabbage (Brassica rapa L.: ssp. pekinensis). Plant Cell Rep 26:327–336CrossRefPubMedGoogle Scholar
- Panjabi P, Jagannath A, Bisht NC, Padmaja KL, Sharma S, Gupta V, Pradhan AK, Pental D (2008) Comparative mapping of Brassica juncea and Arabidopsis thaliana using Intron Polymorphism (IP) markers: homoeologous relationships, diversification and evolution of the A, B and C Brassica genomes. BMC Genom 9:113–132CrossRefGoogle Scholar
- Raman H, Raman R, Coombes N, Song J, Prangnell R, Bandaranayake C, Tahira R, Sundaramoorthi V, Killian A, Meng J, Dennis ES, Balasubramanian S (2015) Genome-wide association analyses reveal complex genetic architecture underlying natural variation for flowering time in canola. Plant Cell Environ. doi: 10.1111/pce.12644 Google Scholar
- Shao YL, Gao YN, Kong YQ, Wang YP (2013) Study on anti-nutritional compounds in seeds of Brassica napus. Bot Res 2:56–61Google Scholar
- Tan ZM, Li YC, Hu Q, Mei DS, Li YD, Xu YS (2007) Heterosis prediction based on genetic distance estimated by molecular markers in rapeseed. Chin J Oil Crop Sci 29(2):20–26Google Scholar
- Wang S, Bastern JC, Zeng ZB (2006) Windows QTL Cartographer 2.5. North Carolina State University, Raleigh. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
- Yao YM, Xu L, Hu Q, Du DZ (2008) Genetic diversity on spring-planted varieties of B. napus L. and their parents. Acta Agron Sin 17(4):114–118Google Scholar