Advertisement

Molecular Breeding

, 35:133 | Cite as

Multiple structural aberrations and physical mapping of rye chromosome 2R introgressed into wheat

  • Lifang Zhuang
  • Peng Liu
  • Zhenqian Liu
  • Tingting Chen
  • Nan Wu
  • Ling Sun
  • Zengjun Qi
Article

Abstract

Multiple structural aberrations produced by chromosome breakage and reunion not only provide new germplasm for enriching genetic diversity but are also helpful for physical mapping of alien chromosomes introgressed into wheat. In this study, mass structural aberrations of rye chromosome 2R were induced by means of spontaneous breakage and reunion, gametocidal chromosome action and irradiation. A total of 88 chromosome 2R aberrations were identified in 65 plants. From the self-pollinated progenies of plants carrying these aberrations, 19 stable lines with different segments of chromosome 2R which included seven whole arm, six small segmental and three large segmental translocations, one deletion and two ditelosomic additions, were subsequently identified and characterized using cytogenetic and molecular markers. Based on these lines, 88 markers specific for chromosome 2R were physically mapped to 13 different blocks of 2R with three in arm 2RS and 10 in arm 2RL. The powdery mildew resistance gene PmJZHM2RL was located to a region corresponding to the block 2RL-7. A total of eighteen 2R-specific EST markers were located in the same block where ten were derived from genes that were up-regulated during powdery mildew infection. Potential use of these stable aberrations and the colinearity of chromosome 2R with corresponding chromosomes in the other model monocot species were discussed.

Keywords

Physical mapping Gene location Powdery mildew resistance Translocations Diagnostic markers 

Notes

Acknowledgments

This project was supported by the National 863 Program of China (2012AA101105), the 111 project (B08025) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Thanks to Dr. B. S. Gill, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA, for providing clones of pSc119.2, pAs1, 45S rDNA and 676D4, Dr. Michael G. Francki, Department of Agriculture and Value Added Wheat Cooperative Research Centre, Bentley, Western Australia, for donating wheat EDM primers and Dr. P. Wehling, Federal Centre for Breeding Research on Cultivated Plants, Gross Lusewitz, Germany, for providing rye SCM primer sequences. Thanks to Dr. C. G. Chu, Monsanto Company, St Louis, Mo, USA, and R. McIntosh, University of Sydney, NSW, Australia, for critical review and suggestions on improving the manuscript.

Supplementary material

11032_2015_333_MOESM1_ESM.doc (336 kb)
Supplementary material 1 (DOC 337 kb)
11032_2015_333_MOESM2_ESM.tif (618 kb)
Supplementary material 2 (TIFF 618 kb)
11032_2015_333_MOESM3_ESM.tif (984 kb)
Supplementary material 3 (TIFF 985 kb)
11032_2015_333_MOESM4_ESM.tif (507 kb)
Supplementary material 4 (TIFF 508 kb)

References

  1. Ashida T, Nasuda S, Sato K, Endo TR (2007) Dissection of barley chromosome 5H in common wheat. Genes Genet Syst 82:123–133PubMedCrossRefGoogle Scholar
  2. Bie TD, Cao YP, Chen PD (2007) Mass production of intergeneric chromosomal translocations through pollen irradiation of Triticum durum-Haynaldia villosa amphiploid. J Integr Plant Biol 49:1619–1626CrossRefGoogle Scholar
  3. Cai XW (1994) Chromosome analysis and C-banded karyotype of “Jingzhouheimai” (Secale cereale). J Huazhong Agric Univ 13:90–92 in Chinese with English abstract Google Scholar
  4. Cao A, Xing L, Wang X, Yang X, Wang W, Sun Y, Qian C, Ni J, Chen Y, Liu D, Wang X, Chen P (2011) Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA 108:7727–7732PubMedCentralPubMedCrossRefGoogle Scholar
  5. Chen TT (2010) Development and molecular marker analysis of chromosome 2R variations of Secale cereale cv. Jingzhouheimai. MS thesis, Nanjing Agricultural University, Nanjing, ChinaGoogle Scholar
  6. Chen SW, Chen PD, Wang XE (2008) Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line. Sci China C Life Sci 51:346–352PubMedCrossRefGoogle Scholar
  7. Cuadrado A, Cardoso M, Jouve N (2008) Physical organisation of simple sequence repeats (SSRs) in Triticeae: structural, functional and evolutionary implications. Cytogenet Genome Res 120:210–219PubMedCrossRefGoogle Scholar
  8. Devos KM, Atkinson MD, Chinoy CN, Francis HA, Harcourt RL, Koebner RMD, Liu CJ, Masojć P, Xie DX, Gale MD (1993) Chromosomal rearrangements in the rye genome relative to that of wheat. Theor Appl Genet 85:673–680PubMedCrossRefGoogle Scholar
  9. Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307CrossRefGoogle Scholar
  10. Friebe B, Jiang J, Gill BS, Dyck PL (1993) Radiation-induced nonhomoeologous wheat-Agropyron intermedium chromosomal translocations conferring resistance to leaf rust. Theor Appl Genet 86:141–149PubMedGoogle Scholar
  11. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  12. Friebe B, Kynast RG, Gill BS (2000) Gametocidal factor-induced structural rearrangements in rye chromosomes added to common wheat. Chromosome Res 8:501–511PubMedCrossRefGoogle Scholar
  13. Gustafson JP, Ma X-F, Korzun V, Snape JW (2009) A consensus map of rye integrating mapping data from five mapping populations. Theor Appl Genet 118:793–800PubMedCrossRefGoogle Scholar
  14. Gyawali YP, Nasuda S, Endo TR (2009) Cytological dissection and molecular characterization of chromosome 1R derived from ‘Burgas 2’ common wheat. Genes Genet Syst 84:407–416PubMedCrossRefGoogle Scholar
  15. Gyawali YP, Nasuda S, Endo TR (2010) A cytological map of the short arm of rye chromosome 1R constructed with 1R dissection stocks of common wheat and PCR-based markers. Cytogenet Genome Res 129:224–233PubMedCrossRefGoogle Scholar
  16. Hackauf B, Wehling P (2003) Development of microsatellite markers in rye: map construction. Plant Breed Seed Sci 48:143–151Google Scholar
  17. Hackauf B, Rudd S, van der Voort JR, Miedaner T, Wehling P (2009) Comparative mapping of DNA sequences in rye (Secale cereale L.) in relation to the rice genome. Theor Appl Genet 118:371–384PubMedCrossRefGoogle Scholar
  18. Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223CrossRefGoogle Scholar
  19. Jiang J, Gill BS (1993) Sequential chromosome banding and in situ hybridization analysis. Genome 36:792–795PubMedCrossRefGoogle Scholar
  20. Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212CrossRefGoogle Scholar
  21. Joshi GP, Nasuda S, Endo TR (2011) Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat. Genes Genet Syst 86:231–248PubMedCrossRefGoogle Scholar
  22. Khlestkina EK, Than MHM, Pestsova EG, Röder MS, Malyshev SV, Korzun V, Börner A (2004) Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor Appl Genet 109:725–732PubMedCrossRefGoogle Scholar
  23. Koebner RMD, Shepherd KW (1985) Induction of recombination between rye chromosome 1RL and wheat chromosomes. Theor Appl Genet 71:208–215PubMedGoogle Scholar
  24. Korzun V, Malyshev S, Voylokov AV, Börner A (2001) A genetic map of rye (Secale cereale L.) combining RFLP, isozyme, protein, microsatellite and gene loci. Theor Appl Genet 102:709–717CrossRefGoogle Scholar
  25. Li AX, Qi ZJ, Pei ZY, Zhuang LF, Feng YG, Wang XE (2007) Development and WSSMV resistance identification of wheat landrace Huixianhong alien chromosome lines derived from rye cultivar Jingzhouheimai. Acta Agron Sin 33:639–645 in Chinese with English abstract Google Scholar
  26. Liu ZQ (2012) Development and identification of chromosome variations of Secale cereale cv. Jingzhouheimai. MS thesis, Nanjing Agricultural University, Nanjing, ChinaGoogle Scholar
  27. Loarce Y, Hueros G, Ferrer E (1996) A molecular linkage map of rye. Theor Appl Genet 93:1112–1118PubMedCrossRefGoogle Scholar
  28. Luan Y, Wang X, Liu W, Li C, Zhang J, Gao A, Wang Y, Yang X, Li L (2010) Production and identification of wheat-Agropyron cristatum 6P translocation lines. Planta 232:501–510PubMedCrossRefGoogle Scholar
  29. Lukaszewski AJ (2000) Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225CrossRefGoogle Scholar
  30. Lukaszewski AJ, Rybka K, Korzun V, Malyshev SV, Lapinski B, Whitkus R (2004) Genetic and physical mapping of homoeologous recombination points involving wheat chromosome 2B and rye chromosome 2R. Genome 47:36–45PubMedCrossRefGoogle Scholar
  31. Ma ZQ, Sorrells ME (1995) Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphisms. Crop Sci 35:1137–1143CrossRefGoogle Scholar
  32. Ma X-F, Wanous MK, Houchins K, Rodriguez Milla MA, Goicoechea PG, Wang Z, Xie M, Gustafson JP (2001) Molecular linkage mapping in rye (Secale cereale L). Theor Appl Genet 102:517–523CrossRefGoogle Scholar
  33. Martis MM, Zhou R, Haseneyer G, Schmutzer T, Vrána J, Kubaláková M, König S, Kugler KG, Scholz U, Hackauf B, Korzun V, Schön C-C, Doležel J, Bauer E, Mayer KFX, Stein N (2013) Reticulate evolution of the rye genome. Plant Cell 25:3685–3698PubMedCentralPubMedCrossRefGoogle Scholar
  34. Masojć P, Myśków B, Milczarski P (2001) Extending a RFLP-based genetic map of rye using random amplified polymorphic DNA (RAPD) and isozyme markers. Theor Appl Genet 102:1273–1279CrossRefGoogle Scholar
  35. Masoudi-Nejad A, Nasuda S, Bihoreau MT, Waugh R, Endo TR (2005) An alternative to radiation hybrid mapping for large-scale genome analysis in barley. Mol Gen Genomics 274:589–594CrossRefGoogle Scholar
  36. McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat, 12th international wheat genetics symposium, pp 130–140. http://www.shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp
  37. Milczarski P, Banek-Tabor A, Lebiecka K, Stojałowski S, Myšków B, Masojć P (2007) New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 x RXL10 intercross. J Appl Genet 48:11–24PubMedCrossRefGoogle Scholar
  38. Milczarski P, Bolibok-Bragoszewska H, Myšków B, Stojałowski S, Heller-Uszyńska K, Góralska M, Bragoszewski P, Uszyński G, Kilian A, Rakoczy-Trojanowska M (2011) A high density consensus map of rye (Secale cereale L.) based on DArT markers. PLoS ONE 6:e28495. doi: 10.1371/journal.pone.0028495a PubMedCentralPubMedCrossRefGoogle Scholar
  39. Mukai Y, Nakahara Y, Yamamoto M (1993) Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome 36:489–494PubMedCrossRefGoogle Scholar
  40. Nasuda S, Kikkawa Y, Ashida T, Islam AKMR, Sato K, Endo TR (2005) Chromosomal assignment and deletion mapping of barley EST markers. Genes Genet Syst 80:357–366PubMedCrossRefGoogle Scholar
  41. Philipp U, Wehling P, Wricke G (1994) A linkage map of rye. Theor Appl Genet 88:243–248PubMedCrossRefGoogle Scholar
  42. Qi ZJ, Zhuang LF, Liu DJ, Chen PD (2000) Transfer of germplasm from Secale cereale cv Jingzhouheimai into cultivated wheat. J Nanjing Agric Univ 23:1–4 in Chinese with English abstract Google Scholar
  43. Ren LJ, Chen PD, Chen HG, Ma HX (2010) Screening of resistance to sharp eyespot in wheat. J Plant Genet Resour 11:108–111 in Chinese with English abstract Google Scholar
  44. Sakai K, Nasuda S, Sato K, Endo TR (2009) Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps. Genes Genet Syst 84:25–34PubMedCrossRefGoogle Scholar
  45. Sakata M, Nasuda S, Endo TR (2010) Dissection of barley chromosome 4H in common wheat by the gametocidal system and cytological mapping of chromosome 4H with EST markers. Genes Genet Syst 85:19–29PubMedCrossRefGoogle Scholar
  46. Senft P, Wricke G (1996) An extended genetic map of rye (Secale cereale L.). Plant Breed 115:508–510CrossRefGoogle Scholar
  47. Sheng BQ, Duan XY (1991) Improvement of scale 0–9 method for scoring adult plant resistance for powdery mildew of wheat. Beijing Agri Sci 1:38–39 in Chinese with English abstract Google Scholar
  48. Shi F, Endo TR (1999) Genetic induction of structural changes in barley chromosomes added to common wheat by a gametocidal chromosome derived from Aegilop cylindrica. Genes Genet Syst 74:49–54CrossRefGoogle Scholar
  49. Shi F, Endo TR (2000) Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat. Chromosoma 109:358–363PubMedCrossRefGoogle Scholar
  50. Szakács É, Kruppa K, Molnár I, Molnár-Láng M (2010) Induction of wheat/barley translocations by irradiation and their detection using fluorescence in situ hybridization. Acta Agron Hung 58:203–209CrossRefGoogle Scholar
  51. Tsuchida M, Fukushima T, Nasuda S, Ali Masoudi-Nejad, Ishikawa G, Endo TR (2008) Dissection of rye chromosome 1R in common wheat. Genes Genet Syst 83:43–53PubMedCrossRefGoogle Scholar
  52. Wang YZ (2013) Development and characterization of small segmental translocations of Thinopyrum bessarabicum and cytological mapping of interest genes. MS thesis, Nanjing Agricultural University, Nanjing, ChinaGoogle Scholar
  53. Wang L, Chen P (2008) Development of Triticum aestivum-Leymus racemosus ditelosomic substitution line 7Lr#1S (7A) with resistance to wheat scab and its meiotic behavior analysis. Chin Sci Bull 53:3522–3529CrossRefGoogle Scholar
  54. Wang D, Zhuang LF, Sun L, Feng YG, Pei ZY, Qi ZJ (2010) Allocation of a powdery mildew resistance locus to the chromosome arm 6RL of Secale cereale L. cv. ‘Jingzhouheimai’. Euphytica 176:157–166CrossRefGoogle Scholar
  55. Wang CL, Zhuang LF, Qi ZJ (2012) Structural variations of chromosome 1R from rye cultivar Jingzhouheimai induced by irradiation. J Nucl Agric Sci 26:28–31 in Chinese with English abstract Google Scholar
  56. Zhang P, Li WL, Friebe B, Gill BS (2004) Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome 47:979–987PubMedCrossRefGoogle Scholar
  57. Zheng Q, Li B, Zhang X, Mu S, Zhou H, Li Z (2006) Molecular cytogenetic characterization of wheat-Thinopyrum ponticum translocations bearing blue-grained gene(s) induced by r-ray. Euphytica 152:51–60CrossRefGoogle Scholar
  58. Zhuang LF, Sun L, Li AX, Chen TT, Qi ZJ (2011) Identification and development of diagnostic markers for a powdery mildew resistance gene on chromosome 2R of Chinese rye cultivar Jingzhouheimai. Mol Breed 27:455–465CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Lifang Zhuang
    • 1
  • Peng Liu
    • 1
  • Zhenqian Liu
    • 1
  • Tingting Chen
    • 1
    • 2
  • Nan Wu
    • 1
  • Ling Sun
    • 1
    • 3
  • Zengjun Qi
    • 1
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingChina
  2. 2.State Key Laboratory of Cotton BiologyInstitute of Cotton Research of Chinese Academy of Agricultural SciencesAnyangChina
  3. 3.School of Food and Biological EngineeringJiangsu UniversityZhenjiangChina

Personalised recommendations