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Molecular Breeding

, 35:78 | Cite as

Mapping of qBK1, a major QTL for bakanae disease resistance in rice

  • Yeon-Jae Hur
  • Sais Beul Lee
  • Tae Heon Kim
  • Tackmin Kwon
  • Jong-Hee Lee
  • Dong-Jin Shin
  • Soo-Kwon Park
  • Un-Ha Hwang
  • Jun Hyeon Cho
  • Young-Nam Yoon
  • Un-Sang Yeo
  • You-Chun Song
  • Do-Yeon Kwak
  • Min-Hee Nam
  • Dong-Soo Park
Article

Abstract

Bakanae disease is caused by several species of Fusarium and imposes serious limitations on rice production worldwide. The incidence of this disease is increasing in the top rice-growing countries. Thus, higher resistance to this disease may be a cost-saving solution preferable to the application of fungicides. Here, we developed 168 near-isogenic rice lines (NILs, BC6F4) to locate a QTL for resistance against bakanae disease. The lines were derived from a cross between Shingwang, a highly resistant variety (indica), and Ilpum, a highly susceptible variety (japonica). The 24 markers representing the Shingwang allele in a bakanae disease-resistant NIL, YR24982-9-1 (parental line of the BC6F4 NILs), were located on chromosome 1, 2, 7, 8, 10, 11, and 12. Single marker analysis using an SSR marker, RM9, showed that a major QTL is located on chromosome 1. The QTL explained 65 % of the total phenotype variation in BC6F4 NILs. The major QTL that designated qBK1 was mapped in 520 kb region between RM8144 and RM 11295. The identification of qBK1 and the closely linked SSR marker, RM9, could be useful for improving rice bakanae disease resistance in marker-assisted breeding.

Keywords

Oryza sativa QTL mapping Bakanae disease Resistance QTL Fusarium fujikuroi 

Notes

Acknowledgments

This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (Project title: QTL mapping and development of elite line for bakanae disease resistance in rice, Project No. PJ01112702)”, Rural Development Administration, Republic of Korea.

Supplementary material

11032_2015_281_MOESM1_ESM.ppt (462 kb)
Supplementary material 1 (PPT 462 kb)
11032_2015_281_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 16 kb)

References

  1. Carter LL, Leslie JF, Webster RK (2008) Population structure of Fusarium fujikuroi from California rice and water grass. Phytopathology 98:992–998CrossRefPubMedGoogle Scholar
  2. Desjardins AE, Manandhar HK, Plattner RD, Manandhar GG, Poling SM, Maragos CM (2000) Fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species. Appl Environ Microbiol 66:1020–1025CrossRefPubMedCentralPubMedGoogle Scholar
  3. Frisch M, Bohn M, Melchinger AE (1999) Comparison of selection strategies for marker-assisted backcrossing of a gene. Crop Sci 39:1295–1301CrossRefGoogle Scholar
  4. Hayasaka T, Ishiguro K, Shibutani K, Namai T (2001) Seed disinfection using hot water immersion to control several seed-borne diseases of rice plants. Jpn J Phytopathol 67:26–32CrossRefGoogle Scholar
  5. Hospital F, Charcosset A (1997) Marker-assisted introgression of quantitative trait loci. Genetics 147:1469–1485PubMedCentralPubMedGoogle Scholar
  6. Hu ZB, Keceli MA, Piisila M, Li J, Survila M, Heino P, Brader G, Palva ET, Li J (2012) F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity. Cell Res 22:777–781CrossRefPubMedCentralPubMedGoogle Scholar
  7. Hwang IS, Kang WR, Hwang DJ, Bae SC, Yun SH, Ahn IP (2013) Evaluation of bakanae disease progression caused by Fusarium fujikuroi in Oryza sativa L. J Microbiol 51:858–865CrossRefPubMedGoogle Scholar
  8. Iqbal M, Javed N, Sahi ST, Cheema NM (2011) Genetic management of bakanae disease of rice and evaluation of various fungicides against Fusarium moniliforme in vitro. Pak J Phytopathol 23:103–107Google Scholar
  9. Ito S, Kimura J (1931) Studies on the ‘bakanae’ disease of the rice plant. Rep Hokkaido Natl Agric Exp Stat 27:1–95Google Scholar
  10. Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-box proteins in rice. Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. Plant Physiol 143:1467–1483CrossRefPubMedCentralPubMedGoogle Scholar
  11. Khokhar LK, Jaffrey AH (2002) Identification of sources of resistance against bakanae and foot rot disease in rice. Pak J Agric Res 17:176–177Google Scholar
  12. Kim JM, Hong SK, Kim WG, Lee YK, Yu SH, Choi HW (2010) Fungicide resistance of gibberella fujikuroi isolates causing rice bakanae disease and their progeny isolates. Kor J Mycol 38:75–79CrossRefGoogle Scholar
  13. Kim MH, Hur YJ, Lee SB, Kwon TM, Hwang UH, Park SK, Yoon YN, Lee JH, Cho JH, Shin DJ, Kim TH, Han SI, Yeo US, Song YC, Nam MH, Park DS (2014) Large-scale screening analysis for the evaluation of bakanae disease in rice. J Gen Plant Pathol. doi: 10.1007/s10327-014-0528-0 Google Scholar
  14. Kwon T, Lee JH, Park SK, Hwang UH, Cho JH, Kwak DY, Youn YN, Yeo US, Song YC, Nam JS, Kang HW, Nam MH, Park DS (2012) Fine mapping and identification of candidate rice genes associated with qSTV11 SG, a major QTL for rice stripe disease resistance. Theor Appl Genet 125:1033–1046CrossRefPubMedGoogle Scholar
  15. Lee YH, Lee MJ, Choi HW, Kim ST, Park JW, Myung IS, Park K, Lee SW (2011) Development of in vitro seedling screening method for selection of resistant rice against bakanae disease. Res Plant Dis 17:288–294CrossRefGoogle Scholar
  16. Li D, Luo K (1997) Study of the relationship between the occurrence of bakanae disease in hybrid rice and the application of gibberellin to seed reproduction. J Hunan Agric Univ 23:47–49Google Scholar
  17. Li D, Luo K, Chen Z (1993) Studies on resistance of rice varieties to bakanae disease and pathogenicity of pathogen Fusarium moniliforme. Acta Phytopathol Sin 23:315–319Google Scholar
  18. Lv B (1994) Preliminary study of identification for resistance to rice bakanae disease in rice resource. Plant Protect 3:20–21Google Scholar
  19. Ma L, Ji Z, Bao J, Zhu X, Li X, Zhuang J, Yang C, Xia Y (2008) Responses of rice genotypes carrying different dwarf genes to Fusarium moniliforme and Gibberellic acid. Plant Prod Sci 11:134–138CrossRefGoogle Scholar
  20. Melchinger A (1990) Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed 104:1–19CrossRefGoogle Scholar
  21. Mourgues F, Brisset MN, Chevreau E (1998) Strategies to improve plant resistance to bacterial diseases through genetic engineering. Trends Biotechnol 16:203–210CrossRefPubMedGoogle Scholar
  22. Nagano H, Onishi K, Ogasawara M, Horiuchi Y, Sano Y (2005) Genealogy of the “Green Revolution” gene in rice. Genes Genet Syst 80:351–356CrossRefPubMedGoogle Scholar
  23. Nelson PE (1981) Fusarium: diseases, biology and taxonomy. The Pennsylvania State University Press, University ParkGoogle Scholar
  24. Nelson JC (1997) QGENE: software for marker-based genomic analysis and breeding. Mol Breed 3:239–245CrossRefGoogle Scholar
  25. Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW, Joyce D (1998) Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol Mol Plant Pathol 53:17–37CrossRefGoogle Scholar
  26. Ogawa K (1988) Damage by bakanae disease and its chemical control. Jpn Pestic Inf 52:13–15Google Scholar
  27. Ou SH (1985) Rice diseases. IRRI, Los BañosGoogle Scholar
  28. Park WS, Choi HW, Han SS, Shin DB, Shim HK, Jung ES, Lee SW, Lim CK, Lee YH (2009) Control of bakanae disease of rice by seed soaking into the mixed solution of procholraz and fludioxnil. Res Plant Dis 15:94–100CrossRefGoogle Scholar
  29. Pra MD, Tonti S, Pancaldi D, Nipoti P, Alberti I (2010) First report of Fusarium andiyazi associated with rice bakanae in Italy. Plant Dis 94:1070CrossRefGoogle Scholar
  30. Rosales AM, Mew TW (1997) Suppression of Fusarium moniliforme in rice by rice-associated antagonistic bacteria. Plant Dis 81:49–52CrossRefGoogle Scholar
  31. Sunder S, Satyavir S (1998) Vegetative compatibility, biosynthesis of GA3 and virulence of Fusarium moniliforme isolates from bakanae disease of rice. Plant Pathol 47:767–772CrossRefGoogle Scholar
  32. Takahashi N, Kitamura H, Kawarada A, Seta Y, Takai M, Tamura S, Sumiki Y (1955) Biochemical studies on bakanae fungus. Bull Agric Chem Soc Jpn 19:267–277CrossRefGoogle Scholar
  33. Thakur KSS (1974) Role of gibberllic acid, fusaric acid anc pectic enzymes in the foot rot disease of rice. Riso 23:191–207Google Scholar
  34. Wahid A, Saleem M, Khan MU, Tariq AR, Saleem A (1993) Seed-borne mycoflora of rice. Pak J Agric Res 31:95–100Google Scholar
  35. Webster RK, Gunnell PS (1992) Compendium of rice diseases. American Phytopathological Society, St. PaulGoogle Scholar
  36. Wulff EG, Sorensen JL, Lubeck M, Nielsen KF, Thrane U, Torp J (2010) Fusarium spp. associated with rice bakanae: ecology, genetic diversity, pathogenicity and toxigenicity. Environ Microbiol 12:649–657CrossRefPubMedGoogle Scholar
  37. Xiang Y, Cao YL, Xu CG, Li XH, Wang SP (2006) Xa3, conferring resistance for rice bacterial blight and encoding a receptor kinase-like protein, is the same as Xa26. Theor Appl Genet 113:1347–1355CrossRefPubMedGoogle Scholar
  38. Yabuta T, Hayasi T (1935) Biochemistry of the bakanae fungus of rice. Agric Hortic 10:17–22Google Scholar
  39. Yamanaka S, Honkura R (1978) Symptoms on rice seedlings inoculated with bakanae disease fungus, Fusarium moniliforme Sheldon. Ann Phytopathol Soc Jpn 44:57–58CrossRefGoogle Scholar
  40. Yan YS, Chen XY, Yang K, Sun ZX, Fu YP, Zhang YM, Fang RX (2011) Overexpression of an F-box protein gene reduces abiotic stress tolerance and promotes root growth in rice. Mol Plant 4:190–197CrossRefPubMedGoogle Scholar
  41. Yang H, Wang Z, Wu H, Zhu C (2003) Study on the variation of rice bakanae under the different methods of seedling raising. J Anhui Agric Sci 31:119–124Google Scholar
  42. Yang CD, Guo LB, Li XM, Ji ZJ, Ma LY, Qian Q (2006) Analysis of QTLs for resistance to rice bakanae disease. Chin J Rice Sci 6:657–659Google Scholar
  43. Yokotani N, Sato Y, Tanabe S, Chujo T, Shimizu T, Okada K, Yamane H, Shimono M, Sugano S, Takatsuji H, Kaku H, Minami E, Nishizawa Y (2013) WRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance. J Exp Bot 64:5085–5097CrossRefPubMedCentralPubMedGoogle Scholar
  44. Zainudin NIM, Izzati NA, Salleh B (2010) Variability of Fusarium species associated with bakanae disease of rice based on their virulence, vegetative and biological compatibilities. Sydowia 62:89–104Google Scholar
  45. Zhu LH, Li X (1984) Heredity of dwarfism in Japonica. Acta Nanjing Agric Coll 4:1–5Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Yeon-Jae Hur
    • 1
  • Sais Beul Lee
    • 1
  • Tae Heon Kim
    • 1
  • Tackmin Kwon
    • 3
  • Jong-Hee Lee
    • 2
  • Dong-Jin Shin
    • 1
  • Soo-Kwon Park
    • 1
  • Un-Ha Hwang
    • 1
  • Jun Hyeon Cho
    • 1
  • Young-Nam Yoon
    • 1
  • Un-Sang Yeo
    • 1
  • You-Chun Song
    • 1
  • Do-Yeon Kwak
    • 2
  • Min-Hee Nam
    • 1
  • Dong-Soo Park
    • 1
  1. 1.National Institute of Crop ScienceMilyangKorea
  2. 2.Research Policy Bureau, RDAJeonjuKorea
  3. 3.Dong-A UniversityPusanKorea

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