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Molecular Breeding

, 35:110 | Cite as

Genetic analysis and gene mapping of papaya ring spot virus resistance in cucumber

  • Guili Tian
  • Yuhong Yang
  • Shengping Zhang
  • Han Miao
  • Hongwei Lu
  • Ye Wang
  • Bingyan Xie
  • Xingfang Gu
Article

Abstract

The papaya ring spot virus (PRSV) causes significant fruit yield loss in cucurbit crops. Understanding of the inheritance and molecular mapping of PRSV resistance will facilitate development of resistant varieties to control this disease. In the present study, an F2 population was developed from the cross between susceptible ‘65G’ and resistant ‘02245’ cucumber inbred lines. Genetic analysis of PRSV resistance in 144 F2:3-derived F3 families showed that resistance is controlled by a single recessive gene which was designated as prsv 02245 . Simple sequence repeat (SSR) markers were employed in polymorphism screening between PRSV-susceptible and resistant DNA pools. The PRSV resistance gene, prsv 02245 , was mapped on chromosome 6 that was flanked by two SSR markers, SSR11-177 and SSR11-1, which was 1.1 and 2.9 cM away from the prsv 02245 locus, respectively. The physical distance between the two markers was approximately 600 kb. The accuracy rate of marker-assisted selection of PRSV resistance among 35 cucumber lines using the marker, SSR11-177 was more than 80 %. Results from this study provide a valuable tool for fine mapping, gene cloning, and marker-assisted breeding for PRSV resistance in cucumber.

Keywords

Cucumber Papaya ring spot virus Virus resistance Gene mapping 

Notes

Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program, No. 2012AA100103); the earmarked fund for Modern Agro-industry Technology Research System (CARS-25) and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China. The authors would like to thank Dr. Graham Collins, formerly of the University of Adelide, South Australia for proofreading.

Supplementary material

11032_2015_279_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Guili Tian
    • 1
  • Yuhong Yang
    • 1
  • Shengping Zhang
    • 1
  • Han Miao
    • 1
  • Hongwei Lu
    • 1
  • Ye Wang
    • 1
  • Bingyan Xie
    • 1
  • Xingfang Gu
    • 1
  1. 1.Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijingChina

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