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Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea)

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Abstract

Anthocyanins have several biological functions in plants and are beneficial to human health. To elucidate the metabolic profile of anthocyanins and determine the genetic basis controlling anthocyanin accumulation in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea), we conducted anthocyanin profile characterization and quantitative trait locus (QTL) analysis. Seventeen anthocyanin compounds were identified as cyanidin glycosides in zicaitai. A genetic linkage map based on 200 F2 lines was constructed using 161 insertion/deletion markers. Total anthocyanin content (TAC) was determined by pH differential spectrophotometry for the F2 lines. Using the map and phenotypic data, a major QTL which explained 56.7 % of phenotypic variation was identified for TAC on chromosome A09. Two genes, BrEGL3.1 and BrEGL3.2, as syntenic orthologs of AtEGL3 encoding basic helix–loop–helix transcription factors in this QTL region, are candidate genes for a key role in the control of anthocyanin accumulation in zicaitai.

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Acknowledgments

The work was funded by the National High Technology R&D Program of China (2012AA100101); the National Program on Key Basic Research Projects of China (The 973 Program: 2012CB113900, 2013CB127000, and 2013CB127006); the International Joint Research Grant of Ministry of Science and Technology, P.R. China (2011DFR31180); and the National Natural Science Foundation of China (NSFC Grants: 31301771, 31201628, 31201636 and 31301784); the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R. China and the Sino-Dutch Joint Lab of Horticultural Genomics Technology as well as the National Engineering Research Center for Vegetables in Beijing.

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Authors and Affiliations

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, Nandajie No. 12, Haidian District, Beijing, 100081, People’s Republic of China

    Ning Guo, Jian Wu, Shuning Zheng, Feng Cheng, Bo Liu, Jianli Liang, Yang Cui & Xiaowu Wang

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  1. Ning Guo
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  2. Jian Wu
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  3. Shuning Zheng
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  4. Feng Cheng
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  5. Bo Liu
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  6. Jianli Liang
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  7. Yang Cui
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  8. Xiaowu Wang
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Correspondence to Xiaowu Wang.

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Supplemental Table S1 The detail informantion of the newly designed 300 InDel markers in this study (XLSX 39 kb)

11032_2015_237_MOESM2_ESM.jpg

Supplementary Fig. S1 Amino acid sequence alignment and comparison of BrEG3.1 and BrEGL3.2 showing amino acid substitutions of the parental lines. Identical residues are highlighted on a black background, and similar residues are highlighted on a gray background (JPEG 329 kb)

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Guo, N., Wu, J., Zheng, S. et al. Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea). Mol Breeding 35, 113 (2015). https://doi.org/10.1007/s11032-015-0237-1

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