Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea)
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Anthocyanins have several biological functions in plants and are beneficial to human health. To elucidate the metabolic profile of anthocyanins and determine the genetic basis controlling anthocyanin accumulation in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea), we conducted anthocyanin profile characterization and quantitative trait locus (QTL) analysis. Seventeen anthocyanin compounds were identified as cyanidin glycosides in zicaitai. A genetic linkage map based on 200 F2 lines was constructed using 161 insertion/deletion markers. Total anthocyanin content (TAC) was determined by pH differential spectrophotometry for the F2 lines. Using the map and phenotypic data, a major QTL which explained 56.7 % of phenotypic variation was identified for TAC on chromosome A09. Two genes, BrEGL3.1 and BrEGL3.2, as syntenic orthologs of AtEGL3 encoding basic helix–loop–helix transcription factors in this QTL region, are candidate genes for a key role in the control of anthocyanin accumulation in zicaitai.
KeywordsAnthocyanin profile QTL EGL3 Brassica rapa
The work was funded by the National High Technology R&D Program of China (2012AA100101); the National Program on Key Basic Research Projects of China (The 973 Program: 2012CB113900, 2013CB127000, and 2013CB127006); the International Joint Research Grant of Ministry of Science and Technology, P.R. China (2011DFR31180); and the National Natural Science Foundation of China (NSFC Grants: 31301771, 31201628, 31201636 and 31301784); the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R. China and the Sino-Dutch Joint Lab of Horticultural Genomics Technology as well as the National Engineering Research Center for Vegetables in Beijing.
- Andersen OM, Markham KR (2006) Flavonoids: chemistry, biochemistry and applications. CRC Press, Taylor & Francis, Boca Raton. 397-398Google Scholar
- Harborne JJB, Baxter H, Moss GP (1999) Phytochemical dictionary: a handbook of bioactive compounds from plants, 2nd edn. CRC Press, Boca Raton, pp 361–363Google Scholar
- Liu J, Wang W, Zhang D, Yu S, Zhang F, Zhao X, Yu J, Lu G (2013b) Primary mapping of pur, a gene controlling purple leaf color in Brassica rapa. Acta Agric Boreal Sin 28(1):49–53 (in Chinese)Google Scholar
- Mazza G, Miniati E (1993) Anthocyanins in fruits, vegetables, and grains. CRC Press, Boca RatonGoogle Scholar
- Park KI, Ishikawa N, Morita Y, Choi JD, Hoshino A, Iida S (2007) A bHLH regulatory gene in the common morning glory, Ipomoea purpurea, controls anthocyanin biosynthesis in flowers, proanthocyanidin and phytomelanin pigmentation in seeds, and seed trichome formation. Plant J 49(4):641–654CrossRefPubMedGoogle Scholar
- Paz-Ares J, Ghosal D, Wienand U, Peterson P, Saedler H (1987) The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. The EMBO J 6(12):3553Google Scholar
- Van Ooijen JW (2004) MapQTL5, software for mapping of quantitative trait loci in experimental populations. Kyazma BV, WageningenGoogle Scholar
- Van Ooijen JW (2006) JoinMap4, software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, WageningenGoogle Scholar
- van Poppel G, Verhoeven DT, Verhagen H, Goldbohm RA (1999) Brassica vegetables and cancer prevention. In: Zappia V, Della Ragione F, Barbarisi A, Russo GL, Iacovo RD (eds) Advances in nutrition and cancer 2. Springer, pp 159–168Google Scholar