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Molecular Breeding

, 35:7 | Cite as

Identification and fine mapping of pm5.1: a recessive gene for powdery mildew resistance in cucumber (Cucumis sativus L.)

  • Jingtao Nie
  • Huanle He
  • Jialin Peng
  • Xuqin Yang
  • Beibei Bie
  • Junlong Zhao
  • Yunli Wang
  • Longting Si
  • Jun-Song Pan
  • Run Cai
Article

Abstract

Powdery mildew is one of the most important diseases of cucumber (Cucumis sativus L.). For many years, researchers have studied and attempted to isolate resistance genes for this disease for breeding powdery mildew-resistant cucumber. In this study, bulked segregant analysis and an initial quantitative trait loci (QTLs) analysis based on a local linkage map were performed using an F2 population. A major locus on the long arm of chromosome 5 was identified for powdery mildew resistance and named pm5.1. For fine mapping of the pm5.1 locus, BC3F1 and BC2F2 populations that segregated only at the target genomic region were developed by marker-assisted selection. The location of the pm5.1 locus was confirmed by QTL analysis using a subset of the BC3F1 population and was delimited in a 1.8-cM interval between flanking markers UW065011 and UW065190. Using the large BC3F1 (n = 480) and BC2F2 (n = 483) populations, pm5.1 was mapped to a ~170-kb region between markers UW065021 and UW065094. Based on the annotations for the genes in this region, a MLO-like gene was identified as the most likely candidate for the pm5.1. Sequence alignment analysis of the MLO-like genes from susceptible and resistant cucumber lines revealed two types of mutations in this candidate gene that may result in recessive resistance to powdery mildew in the resistant cucumber lines.

Keywords

Cucumber (Cucumis sativus L.) Powdery mildew Fine mapping Recessive resistance MLO-like candidate gene QTL 

Notes

Acknowledgments

We thank Lihuang Zhu (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences) for technical assistance. We thank Dr. Sanwen Huang (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) and Dr. Yiqun Weng (University of Wisconsin) for providing the cucumber genome data. We also thank Dr. Dehua Ma (Tianjin Derit Seeds Company Ltd) for providing the cucumber material. This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA100104), the National Key Basic Research Projects (973 Program) (No. 2012CB113900), the National Natural Science Foundation of China (No. 31471892), and Shanghai Graduate Education and Innovation Program (Horticulture).

Supplementary material

11032_2015_206_MOESM1_ESM.doc (364 kb)
Supplementary material 1 (DOC 363 kb)

References

  1. Büschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J, Töpsch S, Vos P, Salamini F, Schulze-Lefert P (1997) The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695–705CrossRefPubMedGoogle Scholar
  2. Cavagnaro PF, Senalik DA, Yang L, Simon PW, Harkins TT, Kodira CD, Huang S, Weng Y (2010) Genome-wide characterization of simple sequence repeats in cucumber (Cucumis sativus L.). BMC Genomics 15:569CrossRefGoogle Scholar
  3. Consonni C, Humphry ME, Hartmann HA, Livaja M, Durner J, Westphal L, Vogel J, Lipka V, Kemmerling B, Schulze-Lefert P, Somerville SC, Panstruga R (2006) Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nat Genet 38:716–720CrossRefPubMedGoogle Scholar
  4. Darvasi A, Soller M (1994) Selective DNA pooling for determination of linkage between a molecular marker and a quantitative trait locus. Genetics 138:1365–1373PubMedCentralPubMedGoogle Scholar
  5. de Ruiter W, Hofstede R, de Vries J, van den Heuvel H (2008) Combining QTL for resistance to CYSDV and powdery mildew in a single cucumber line. In: Pitrat M (ed) Proceedings of the 9th EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, France, May 21–24, pp 181–188Google Scholar
  6. Fukino N, Ohara T, Monforte A, Sugiyama M, Sakata Y, Kunihisa M, Matsumoto S (2008) Identification of QTLs for resistance to powdery mildew and SSR markers diagnostic for powdery mildew resistance genes in melon(Cucumis melo L.). Theor Appl Genet 118:165–175CrossRefPubMedGoogle Scholar
  7. Fukino N, Yoshioka Y, Sugiyama M, Sakata Y, Matsumoto S (2013) Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativas L.). Mol Breed 32:267–277CrossRefGoogle Scholar
  8. Fukuoka S, Okuno K (2001) QTL analysis and mapping of pi21, a recessive gene for field resistance to rice blast in Japanese upland rice. Theor Appl Genet 103:185–190CrossRefGoogle Scholar
  9. He XM, Li YH, Pandey S, Yandell BS, Pathak M, Weng YQ (2013) QTL mapping of powdery mildew resistance in WI 2757 cucumber (Cucumis sativus L.). Theor Appl Genet 126:2149–2161CrossRefPubMedGoogle Scholar
  10. Hofstede R, de Ruiter W, de Vries RJ, van den Heuvel H (2008) Disease resistant cucumber plants. US Patent (# US 2008/0307540 A1)Google Scholar
  11. Huang SW, Li RQ, Zhang ZH, Li L, Gu XF, Fan W et al (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41:1275–1281CrossRefPubMedGoogle Scholar
  12. Kang HX, Weng YQ, Yang YH, Zhang ZH, Zhang SP, Mao ZC, Cheng GH, Gu XF, Huang SW, Xie BY (2011) Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster. Theor Appl Genet 122:795–803CrossRefPubMedGoogle Scholar
  13. Kosambi D (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175CrossRefGoogle Scholar
  14. Kou YJ, Wang SP (2010) Broad-spectrum and durability: understanding of quantitative disease resistance. Curr Opin Plant Biol 13:181–185CrossRefPubMedGoogle Scholar
  15. Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898CrossRefPubMedGoogle Scholar
  16. Liu LZ, Yuan XJ, Cai R, Pan JS, He HL, Yuan LH, Guan Y, Zhu LH (2008) Quantitative trait loci for resistance to powdery mildew in cucumber under seedling spray inoculation and leaf disc infection. J Phytopathol 156:691–697CrossRefGoogle Scholar
  17. Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832CrossRefPubMedCentralPubMedGoogle Scholar
  18. Morishita M, Sugiyama K, Saito T, Sakata Y (2003) Powdery mildew resistance in cucumber. Jpn Agric Res Q 37(1):7–14CrossRefGoogle Scholar
  19. Murray MG, Thompson WF (1980) Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325CrossRefPubMedCentralPubMedGoogle Scholar
  20. Pavan S (2011) Exploring recessive resistance to the powdery mildew disease. Dissertation, Wageningen UniversityGoogle Scholar
  21. Perchepied L, Bardin M, Dogimont C, Pitrat M (2005) Relationship between loci conferring downy mildew and powdery mildew resistance in melon assessed by quantitative trait loci mapping. Phytopathology 95:556–565CrossRefPubMedGoogle Scholar
  22. Qi JJ, Liu X, Shen D, Miao H, Xie BY, Li XX, Zeng P, Wang SH, Shang Y, Gu XF, Du YC, Li Y, Lin T, Yuan JH, Yang XY, Chen JF, Chen HM, Xiong XY, Huang K, Fei ZJ, Mao LY, Tian L, Thomas S, Susanne SR, Sophien K, Willian JL, Zhang ZH, Huang SW (2013) A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity. Nat Genet. doi: 10.1038/ng.2801 Google Scholar
  23. Qiu YF, Guo JP, Jing SL, Zhu LL, He GC (2010) High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds. Theor Appl Genet 121:1601–1611CrossRefPubMedGoogle Scholar
  24. Ren Y, Zhang Z, Staub J, Cheng Z, Li X, Lu J, Miao H, Kang H, Xie B, Gu X (2009) An integrated genetic and cytogenetic map of the cucumber genome. PLoS One 4:1–8CrossRefGoogle Scholar
  25. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386PubMedGoogle Scholar
  26. Sakata Y, Kubo N, Morishita M, Kitadani E, Sugiyama M, Hirai M (2006) QTL analysis of powdery mildew resistance in cucumber. Theor Appl Genet 112:243–250CrossRefPubMedGoogle Scholar
  27. Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0, software for the calculation of genetic linkage maps. Kyazma BV, WageningenGoogle Scholar
  28. Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. North Carolina State University, Raleigh. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
  29. Wang X, Li G, Gao X, Xiong L, Wang W, Han R (2011) Powdery mildew resistance gene (Pm-AN) located in a segregation distortion region of melon LGV. Euphytica 180(3):421–428CrossRefGoogle Scholar
  30. Wóycicki R, Witkowicz J, Gawroński P, Dąbrowska J, Lomsadze A, Pawełkowicz M, Siedlecka E, Yagi K, Pląder W, Seroczyńska A, Śmiech M, Gutman W, Niemirowicz-Szczytt K, Bartoszewski G, Tagashira N, Hoshi Y, Borodovsky M, Karpiński S, Malepszy S, Przybecki Z (2011) The genome sequence of the North-European cucumber (Cucumis sativus L.) unravels evolutionary adaptation mechanisms in plants. PLoS One 6:e22728CrossRefPubMedCentralPubMedGoogle Scholar
  31. Yang L, Koo DH, Li Y, Zhang X, Luan F, Havey MJ, Jiang J, Weng Y (2012) Chromosome rearrangements during domestication of cucumber as revealed from high-density genetic mapping and draft genome assembly. Plant J 71:895–906CrossRefPubMedGoogle Scholar
  32. Yuste-Lisbona FJ, López-Sesé AI, Gómez-Guillamón ML (2010) Inheritance of resistance to races 1, 2 and 5 of powdery mildew in the melon TGR-1551. Plant Breed 129:72–75CrossRefGoogle Scholar
  33. Yuste-Lisbona FJ, Capel C, Sarria E, Torreblance R, Gómez-Guillamón ML, Capel J, Lozano R, López-Sesé AI, Lozano R (2011a) Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance. Mol Breed 27(2):181–192CrossRefGoogle Scholar
  34. Yuste-Lisbona FJ, Capel C, Gómez-Guillamón ML, Capel J, López-Sesé AI, Lozano R (2011b) Codominant PCR-based markers and candidate genes for powdery mildew resistance in melon(Cucumis melo L.). Theor Appl Genet 122:747–758CrossRefPubMedGoogle Scholar
  35. Zhang HY, Wang ZG, Mao AJ, Zhang F, Wang YJ, Xu Y (2008) SSR markers linked to the resistant gene of cucumber powdery mildew. Acta Agric Boreali Sin 23:77–80Google Scholar
  36. Zhang SP, Liu MM, Miao H, Zhang SQ, Yang YH, Xie BY, Gu XF (2011) QTL mapping of resistance genes to powdery mildew in cucumber. Sci Agric Sin 44:3584–3593Google Scholar
  37. Zhang WW, Pan JS, He HL, Zhang C, Li Z, Zhao JL, Yuan XJ, Zhu LH, Huang SW, Cai R (2012) Construction of a high density integrated genetic map for cucumber (Cucumis sativus L.). Theor Appl Genet 124:249–259CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Jingtao Nie
    • 1
  • Huanle He
    • 1
  • Jialin Peng
    • 1
  • Xuqin Yang
    • 1
  • Beibei Bie
    • 1
  • Junlong Zhao
    • 1
  • Yunli Wang
    • 1
  • Longting Si
    • 2
  • Jun-Song Pan
    • 1
  • Run Cai
    • 1
  1. 1.School of Agriculture and BiologyShanghai Jiaotong UniversityShanghaiChina
  2. 2.Department of HorticultureShenyang Agriculture UniversityShenyangChina

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