Evaluation of juvenile drought stress tolerance and genotyping by sequencing with wild barley introgression lines
- 633 Downloads
Drought is a major stress which can seriously limit yield in many crops including barley. Wild barley introgression lines (ILs) like the S42IL library may enhance drought stress tolerance of barley cultivars through the introduction of exotic alleles. The S42IL library was already characterized with 636 Illumina SNPs. New approaches like genotyping by sequencing (GBS) are available for barley to enhance the characterization of ILs. We generated an improved genetic map of the S42IL library, consisting of 4,201 SNPs by adding GBS data. The new map with a total length of 989.2 cM confirmed the extent of wild barley introgressions. Adding GBS data increased the resolution of the S42IL map tenfold from 0.4 to 4.2 markers/cM. This may assist to select possible candidate genes that improve drought tolerance. In four greenhouse experiments, juvenile drought stress response of 52 barley S42ILs was tested to identify quantitative trait loci (QTL). Thirteen S42ILs showed effects for plant biomass and leaf senescence. Subsequently, two verification experiments were conducted with these S42ILs. Nine out of eleven QTL were verified, and 22 additional QTL were detected. For 21 QTL, the Hsp allele increased trait performance, indicating the value of wild barley introgressions. For example, S42IL-107 and S42IL-123 produced more biomass under drought. Two different water-saving strategies were observed. S42IL-143 and S42IL-129 both revealed increased relative water content under drought. While S42IL-143 reduced biomass under drought, S42IL-129 maintained a high biomass production. We recommend using S42IL-107, S42IL-123 and S42IL-129 in barley breeding programs to enhance drought tolerance.
KeywordsBarley Hordeum vulgare spp. spontaneum Introgression lines Drought tolerance Genotyping by sequencing (GBS)
We are grateful to the team of Australian Genome Research Facility Ltd., St. Lucia, QLD, Australia, for carrying out the sequencing of the S42ILs. This work was supported by the Interdisciplinary Centre for Crop Plant Research (IZN), Halle (Saale) and by the Joint Research Co-operation Scheme, of the German Academic Exchange Service (DAAD, ID 54391967) and the Group of Eight, Australia.
- Becker H (2011) Pflanzenzüchtung, 2nd edn. Verlag Eugen Ulmer, StuttgartGoogle Scholar
- Elshire RJ, Glaubitz JC, Sun Q et al (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS One. doi: 10.1371/journal.pone.0019379
- Institute SAS (2008) The SAS enterprise guide for Windows, release 4.2. SAS Institute, CaryGoogle Scholar
- Jana S, Wilen RW (2005) Breeding for abiotic stress tolerance in barley. In: Ashraf M, Harris PJC (eds) Abiotic stresses: plant resistance through breeding and molecular approaches. Food Products PressGoogle Scholar
- Karsai I, Meszaros K, Szucs P et al (2006) The influence of photoperiod on the Vrn-H2 locus (4H) which is a major determinant of plant development and reproductive fitness traits in a facultative X winter barley (Hordeum vulgare L.) mapping population. Plant Breed 125:468–472. doi: 10.1111/j.1439-0523.2006.01266.x CrossRefGoogle Scholar
- Laurie DA, Pratchett N, Bezant JH, Snape JW (1995) RFLP mapping of 5 major genes and 8 quantitative trait loci controlling flowering time in a winter x spring barley (Hordeum vulgare L.) cross. Genome 38:575–585Google Scholar
- Schmalenbach I, March TJ, Bringezu T et al (2011) High-resolution genotyping of wild barley introgression lines and fine-mapping of the threshability locusthresh-1 using the Illumina GoldenGate Assay. G3 1:187–196. doi: 10.1534/g3.111.000182
- Tyagi K, Park MR, Lee HJ et al (2011a) Fertile crescent region as source of drought tolerance at early stage of plant growth of wild barley (Hordeum vulgare L. ssp. spontaneum). Pak J Bot 43:475–486Google Scholar
- Tyagi K, Park MR, Lee HJ et al (2011b) Diversity for seedling vigor in wild barley (Hordeum vulgare L. subsp spontaneum) Germplasm. Pak J Bot 43:2167–2173Google Scholar
- Wang G, Schmalenbach I, von Korff M et al (2010) Association of barley photoperiod and vernalization genes with QTLs for flowering time and agronomic traits in a BC(2)DH population and a set of wild barley introgression lines. Theor Appl Genet 120:1559–1574. doi: 10.1007/s00122-010-1276-y PubMedCrossRefPubMedCentralGoogle Scholar