OsMsr9, a novel putative rice F-box containing protein, confers enhanced salt tolerance in transgenic rice and Arabidopsis
Salinity is a major environmental stress that limits agricultural production and geographical distribution of plants. In a previous study, it has been shown that OsMsr9 was induced by cold, drought and heat stresses. However, functions of OsMsr9 at physiological and molecular levels are still unknown. Here, we report that OsMsr9 plays roles in salt tolerance in plants. Quantitative real-time PCR (qPCR) analysis revealed that OsMsr9 was also rapidly and strongly induced by salt stress. Overexpression of OsMsr9 in Arabidopsis and rice showed enhanced salt stress tolerance displaying increased shoot and root elongation, higher survival rates in transgenic plants compared with wild type. OsMsr9 might act as a positive regulator of plant salt tolerance with reinforced expression of stress-related genes, such as RD29A, DREB2A and RAB18 in transgenic plants under salt conditions. Furthermore, transgenic plants accumulated more compatible solutes (proline and soluble sugar) and low level of malondialdehyde, alleviating the changes in reactive oxygen species. These results indicate that OsMsr9 could be a useful gene in developing transgenic crops with enhanced salt tolerance.
KeywordsF-box Salt tolerance Overexpression Rice Arabidopsis
This research was supported by National Natural Science Foundation of China (31171536, 31301253).
- Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP (2007) F-Box proteins in rice. Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress. Plant Physiol 143:1467–1483PubMedCrossRefPubMedCentralGoogle Scholar
- Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406PubMedCrossRefPubMedCentralGoogle Scholar
- Lu XD, Song SF, Li LY, Wang ML, Xia XJ (2010) Expression and cloning of a novel stress-responsive gene OsMsr9 in rice. Res Agr Moder 31:228–232Google Scholar
- Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K (2004) Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiol 136:2734–2746PubMedCrossRefPubMedCentralGoogle Scholar
- Székely G, Abrahám E, Cséplo A, Rigó G, Zsigmond L, Csiszár J, Ayaydin F, Strizhov N, Jásik J, Schmelzer E, Koncz C, Szabados L (2008) Duplicated P5CS genes of Arabidopsis play distinct oles in stress regulation and developmental control of proline bio-synthesis. Plant J 53:11–28PubMedCrossRefGoogle Scholar
- Van den Burg HA, Tsitsigiannis DI, Rowland O, Lo J, Rallapalli G, Maclean D, Takken FL, Jones JD (2008) The F-box protein ACRE189/ACIF1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato. Plant Cell 20:697–719PubMedCrossRefPubMedCentralGoogle Scholar
- Xu GY, Cui YC, Li MJ, Wang ML, Yu Y, Zhang B, Huang LF, Xia XJ (2013) OsMSR2, a novel rice calmodulin-like gene, confers enhanced salt tolerance in rice (Oryza sativa L.). Aust J Crop Sci 7(3):368–373Google Scholar