Oil palm (Elaeis guineensis Jacq.) linkage map, and quantitative trait locus analysis for sex ratio and related traits
- 625 Downloads
Sex ratio and shell-thickness type are among the main components determining yield in oil palm. An integrated linkage map of oil palm was constructed based on 208 offspring derived from a cross between two tenera palms differing in inherited sex ratio. The map consisted of 210 genomic simple sequence repeats (SSRs), 28 expressed sequence tag SSRs, 185 amplified fragment length polymorphism markers, and the Sh locus, which controls shell-thickness phenotype, distributed across 16 linkage groups covering 1,931 cM, with an average marker distance of 4.6 cM. Quantitative trait locus (QTL) analysis identified eight QTLs across six linkage groups associated with sex ratio and related traits. These QTLs explained 8.1–13.1 % of the total phenotypic variance. The QTL for sex ratio on linkage group 8 overlapped with a QTL for number of male inflorescences. In most cases a specific QTL allele combination was responsible for genotype class mean differences, suggesting that most QTLs in heterozygous oil palm are likely to be segregating for multiple alleles with different degrees of dominance. In addition, two new SSRs were shown to flank the major Sh locus controlling the fruit variety type in oil palm.
KeywordsOil palm Sex ratio QTL mapping
This study was supported by grants from the National Centre for Genetic Engineering and Biotechnology (BIOTEC), Thailand.
- Beirnaert A, Vanderweyen R (1941) Contribution à l’étude génétique et biométrique des variétés d’Elaeis guineensis Jacq. Publications de l’Institut National pour l’Etude Agronomique du Congo Belge. Série scientifique no 27Google Scholar
- Benbouza H, Jacquemin JM, Baudoin T, Mergeai G (2006) Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol Agron Soc Environ 10:77–81Google Scholar
- Billotte N, Marseillac N, Risterucci AM, Adon B, Brottier P, Baurens FC, Singh R, Herrán A, BillotC A, Amblard P, Durand-Gasselin T, Courtois B, Asmono D, Cheah SC, Rohde W, Ritter E, Charrier A (2005) Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 110:754–765PubMedCrossRefGoogle Scholar
- Billotte N, Jourjon MF, Marseillac N, Berger A, Flori A, Asmady H, Adon B, Singh R, Nouy B, Potier F, Cheah SC, Rohde W, Ritter E, Courtois B, Charrier A, Mangin B (2010) QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 120:1673–1687PubMedCentralPubMedCrossRefGoogle Scholar
- Bishop DT, Cannings C, Skolnick M, Williamson JA (1983) The number of polymorphic clones required to map the human genome. In: Weir BS (ed) Statistical analysis of DNA sequence data. Marcel and Dekker, New York, pp 181–200Google Scholar
- Corley RHV (1977) Oil palm yield components and yield cycles. In: Earp DA, Newall W (eds) International developments in oil palm. Incorporated Society of Planters, Kuala Lumpur, Malaysia, pp 116–129Google Scholar
- Maria M, Clyde MM, Cheah SC (1995) Cytological analysis of Elaeis guineensis (tenera) chromosomes. Elaeis 7:122–134Google Scholar
- Van Ooijen J, Voorrips R (2001) JoinMap v. 3, software in the calculation of genetic linkage maps. Plant Research International, Wageningen, The NetherlandsGoogle Scholar
- Van Ooijen JW, Boer MP, Jansen RC, Maliepaard C (2002) MapQTL 4.0, software for the calculation of QTL positions on genetic maps. Plant Research International, Wageningen, The NetherlandsGoogle Scholar