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Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance

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Abstract

Pre-harvest sprouting (PHS) reduces the quality of wheat (Triticum aestivum L.) and the economic value of the grain. Our previous studies characterized the haplotypes of Viviparous-1B (Vp-1B) and its association with PHS resistance in Chinese wheats. The objective of this study was to analyze the haplotypes of Viviparous-1A (Vp-1A) and Viviparous-1D (Vp-1D) in a collection of 103 widely-grown winter wheats in China, and their associations with PHS resistance. In total, 17 Vp-1A haplotypes were explored on chromosome 3A of bread wheat, and were located in three major regions, the third intron, fifth intron and sixth exon, and designated TaVp-1Aam, TaVp-1Aan, TaVp-1Aao, TaVp-1Abm, TaVp-1Abn, TaVp-1Agm, TaVp-1Ahm, TaVp-1Ahn, TaVp-1Aho, TaVp-1Aim, TaVp-1Ain, TaVp-1Aio, TaVp-1Ajm, TaVp-1Ajn, TaVp-1Akm, TaVp-1Alm and TaVp-1Aln, respectively. However, no allelic variation of Vp-1D was found in this set of germplasm. Based on the haplotypes explored and their average germination index values, a novel co-dominant sequence-tagged site marker of the TaVp-1A gene was developed and designated Vp1A3. In most cases, haplotype TaVp-1Agm was associated with higher resistance to PHS. By combining with our previously exploited Vp1B3 marker, the efficiency of marker-assisted selection for PHS-resistant varieties was improved. Moreover, while the haplotype combination of TaVp-1Aam and TaVp-1Ba was associated with greater PHS susceptibility, the haplotype combinations TaVp-1Agm and TaVp-1Bb, TaVp-1Agm and TaVp-1Ba, TaVp-1Aim and TaVp-1Bb, and TaVp-1Aam and TaVp-1Bb could confer higher PHS resistance and be used as potential parental lines for molecular marker-assisted wheat breeding for PHS resistance.

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Acknowledgments

This project was partly funded by the China National Basic Research Program (2009CB118300), National Natural Science Foundation of China (30960177, 31160247), Natural Science Foundation of Inner Mongolia (2009BS0301) and Innovative Research Group Fund of Inner Mongolia Agriculture University (NDPYTD2010-3).

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Authors and Affiliations

  1. College of Life Science, Inner Mongolia Agricultural University, No. 306 Zhaowuda Road, Hohhot, 010018, Inner Mongolia, China

    Y. Yang, S. X. Liu, Y. Q. Sun & J. Y. Meng

  2. Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS), No. 12 Zhongguancun South Street, Beijing, 100081, China

    C. L. Zhang & L. Q. Xia

  3. Wheat Research Institute, Heilongjiang Academy of Agricultural Sciences, No. 1 Nongye Road, Harbin, 150086, Heilongjiang, China

    C. L. Zhang

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  1. Y. Yang
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  2. C. L. Zhang
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  3. S. X. Liu
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  5. J. Y. Meng
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Correspondence to Y. Yang or L. Q. Xia.

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Y. Yang and C. L. Zhang contributed equally to this work.

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Yang, Y., Zhang, C.L., Liu, S.X. et al. Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance. Mol Breeding 33, 75–88 (2014). https://doi.org/10.1007/s11032-013-9935-8

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