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The effect of pyramiding two potato cyst nematode resistance loci to Globodera pallida Pa2/3 in potato

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Abstract

Globodera pallida is a major nematode pest causing severe constraints in many potato production regions worldwide. The most prevalent G. pallida pathotypes are Pa2 and Pa3, which exist in mixed populations referred to as Pa2/3. Due to heterogeneity for avirulence genes within these nematode populations, so far, breeders have failed to identify a single resistance gene source which offers complete resistance. Currently, there are two quantitative trait loci (QTL) available, \( GpaIV_{adg}^{s} \) and Gpa5, conferring partial levels of resistance to G. pallida pathotype Pa2/3. The objective of this research was to pyramid \( GpaIV_{adg}^{s} \) and Gpa5 through marker-assisted selection to investigate whether or not pyramiding provides increased resistance to G. pallida Pa2/3. We developed a population segregating for both resistance QTLs and, using diagnostic genetic markers (Contig237 and HC), we identified groups of individuals from this population containing each individual QTL, both QTLs simultaneously, and neither QTL. These individuals were assessed for their resistance levels against G. pallida Pa2/3 population Chavornay. We demonstrated that individuals carrying both QTLs showed a significant reduction in the number of cysts formed in comparison to genotypes carrying \( GpaIV_{adg}^{s} \) or Gpa5, indicating an additive effect. Overall these results show that MAS-based pyramiding of these QTLs is an effective strategy for breeding cultivars exhibiting very high levels of resistance to G. pallida pathotype Pa2/3.

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Acknowledgments

This research was funded by the Teagasc Walsh Fellowship Scheme. We would like to acknowledge Dr. Jim Grant for his statistical advice.

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Authors and Affiliations

  1. Teagasc, Crops, Environment and Land Use Programme, Oak Park Research, Carlow, Ireland

    Emmet Dalton, Denis Griffin & Dan Milbourne

  2. School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland

    Emmet Dalton & Thomas F. Gallagher

  3. Averis Seeds B.V., Valthermond, The Netherlands

    Nick de Vetten

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  1. Emmet Dalton
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  2. Denis Griffin
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  3. Thomas F. Gallagher
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  4. Nick de Vetten
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  5. Dan Milbourne
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Correspondence to Dan Milbourne.

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Dalton, E., Griffin, D., Gallagher, T.F. et al. The effect of pyramiding two potato cyst nematode resistance loci to Globodera pallida Pa2/3 in potato. Mol Breeding 31, 921–930 (2013). https://doi.org/10.1007/s11032-013-9845-9

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  • DOI: https://doi.org/10.1007/s11032-013-9845-9

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