Advertisement

Molecular Breeding

, Volume 31, Issue 3, pp 529–536 | Cite as

Markers for breeding heat-tolerant cowpea

  • Mitchell R. Lucas
  • Jeffery D. Ehlers
  • Bao-Lam Huynh
  • Ndeye-Ndack Diop
  • Philip A. Roberts
  • Timothy J. Close
Article

Abstract

The warm-season legume, cowpea (Vigna unguiculata), is an important crop that performs well in marginal environments. The effects of high temperature are among the most substantial challenges faced by growers of cowpea. Heat injury during late reproductive development sterilizes pollen such that no fruit is set. To study the inheritance of this trait and to deliver resources to breed cowpea with enhanced tolerance to heat, we performed a quantitative trait locus (QTL) analysis using 141 individuals from a recombinant inbred population made from a cross between cowpea varieties CB27 and IT82E-18. Five regions, which represent 9 % of the cowpea genome, explain 11.5–18.1 % of the phenotypic variation and are tagged with 48 transcript-derived single nucleotide polymorphism markers. Favorable haplotypes were donated by CB27 for four of these regions while IT82E-18 was the source of tolerance explained by the fifth QTL. Homeologous regions in soybean contain several genes important for tolerance to heat, including heat shock proteins, heat shock transcription factors, and proline transporters. This work presents essential information for marker-assisted breeding and supports previous findings concerning heat-induced male sterility in cowpea.

Keywords

Legume Heat QTL analysis Haplotype Synteny Breeding 

Notes

Acknowledgments

This work was supported by the Generation Challenge Program (GCP) through a grant from the Bill and Melinda Gates Foundation and US Agency for International Development Collaborative Research Support Program GDG-G-00-02-00012-00 and EDH-A-00-07-00005. We acknowledge the collaborative effort led by members of the GCP, the Centro International de Mejoramiento de Maiz y Trigo within the Integrated Breeding Platform, and members of the University of California Riverside cowpea team for the development of the cowpea Kbiosciences genotyping platform and for the implementation of Genotyping Support Services which can be accessed by following the link: http://www.generationcp.org/gss_homepage.

Supplementary material

11032_2012_9810_MOESM1_ESM.docx (96 kb)
Supplementary material 1 (DOCX 97 kb)
11032_2012_9810_MOESM2_ESM.docx (18 kb)
Supplementary material 2 (DOCX 19 kb)
11032_2012_9810_MOESM3_ESM.docx (14 kb)
Supplementary material 3 (DOCX 14 kb)

References

  1. Ahmed FE, Hall AE, DeMason DA (1992) Heat injury during floral development in cowpea (Vigna unguiculata, Fabaceae). Am J Bot 79:784–791CrossRefGoogle Scholar
  2. Choi HK, Mun JH, Kim DJ, Zhu H, Baek JM, Mudge J, Roe B, Ellis N, Doyle J, Kiss GB, Young ND, Cook DR (2004) Estimating genome conservation between crop and model legume species. Proc Natl Acad Sci USA 101:15289–15294PubMedCrossRefGoogle Scholar
  3. Dow El-Medina IM, Hall AE (1986) Flowering of contrasting cowpea (Vigna unguiculata [L.] Walp.) genotypes under different temperatures and photoperiods. Field Crop Res 14:87–104CrossRefGoogle Scholar
  4. Ehlers JD, Hall AE, Patel PN, Roberts PA, Matthews WC (2000) Registration of ‘California Blackeye 27′ cowpea. Crop Sci 40:854–855CrossRefGoogle Scholar
  5. Frova C, Sari-Gorla M (1994) Quantitative trait loci (QTLs) for pollen thermotolerance detected in maize. Mol Gen Genet 245:424–430PubMedCrossRefGoogle Scholar
  6. Hall AE (1992) Breeding for heat tolerance. Plant Breed Rev 10:129–168Google Scholar
  7. Hall AE (2012) Heat stress. In: Shabala S (ed) Plant stress physiology. CABI, Cambridge, pp 118–131CrossRefGoogle Scholar
  8. Mason RE, Mondal S, Beecher FW, Pacheco A, Jampala B, Ibrahim AMH, Hays DB (2010) QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica 174:423–436Google Scholar
  9. Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455PubMedGoogle Scholar
  10. Lamesch P, Berardini TZ, Li D, Swarbreck D, Wilks C, Sasidharan R, Muller R, Dreher K, Alexander DL, Garcia-Hernandez M, Karthikeyan AS, Lee CH, Nelson WD, Ploetz L, Singh S, Wensel A, Huala E (2011) The Arabidopsis information resource (TAIR): improved gene annotation and new tools. Nucleic Acids Res 40:1202–1210CrossRefGoogle Scholar
  11. Lane A, Jarvis A (2007) Changes in climate will modify the geography of crop suitability: agricultural biodiversity can help with adaptation. J SAT Agric Res 4:1–12Google Scholar
  12. Lucas MR, Diop NN, Wanamaker S, Ehlers JD, Roberts PA, Close TJ (2011) Cowpea-soybean synteny clarified through an improved genetic map. Plant Gen 4:218–225CrossRefGoogle Scholar
  13. Marfo KO, Hall AE (1992) Inheritance of heat tolerance during pod set in cowpea. Crop Sci 32:912–918CrossRefGoogle Scholar
  14. Medicago truncatula HapMap Project (2010) Mt3.5 genome assembly annotation November 2010, Nevin Young, University of Minnesota, St. Paul, MN. Available at http://medicagohamap.org/
  15. Muchero W, Diop NN, Bhat PR, Fenton RD, Wanamaker S, Pottorff M, Hearne S, Cisse N, Fatokun C, Ehlers JD, Roberts PA, Close TJ (2009) A consensus genetic map of cowpea [Vigna unguiculata (L) Walp.] and synteny based on EST-derived SNPs. Proc Natl Acad Sci USA 106(43):18159–18164PubMedCrossRefGoogle Scholar
  16. Mutters RG, Ferreira LGR, Hall AE (1989a) Proline content of the anthers and pollen of heat-tolerant and heat-sensitive cowpea subjected to different temperatures. Crop Sci 29:1497–1500CrossRefGoogle Scholar
  17. Mutters RG, Hall AE, Patel PN (1989b) Photoperiod and light quality effects on cowpea floral development at high temperatures. Crop Sci 29:1501–1505CrossRefGoogle Scholar
  18. Nielsen CL, Hall AE (1985) Responses of cowpea (Vigna unguiculata (L.) Walp.) in the field to high night air temperature during flowering. II. Plant Responses. Field Crops Res 10:181–196CrossRefGoogle Scholar
  19. Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, GRimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183PubMedCrossRefGoogle Scholar
  20. Thiaw S, Hall AE (2004) Comparison of selection for either leaf electrolyte-leakage or pod set in enhancing heat tolerance and grain yield of cowpea. Field Crops Res. 86:239–253CrossRefGoogle Scholar
  21. Van Ooijen JW (2004) MapQTL5. Software for the mapping of quantitative trait loci in experimental populations, Kyazma BV, Wageningen, NetherlandsGoogle Scholar
  22. Wahid A, Gelani S, Ashraf M, Foolad MR (2007) Heat tolerance in plants: an overview. Environ Exp Bot 61:199–223CrossRefGoogle Scholar
  23. Wanamaker S and Close TJ (2011) HarvEST. HarvEST:Cowpea version 1.27, University of California Riverside. Available at http://harvest.ucr.edu
  24. Warrag MOA, Hall AE (1984) Reproductive responses of cowpea (Vigna unguiculata [L.] Walp.) to heat stress. II. Responses to night air temperature. Field Crop Res 8:17–33CrossRefGoogle Scholar
  25. Ye C, Argayoso MA, Redona ED, Sierra SN, Laza MA, Dilla CJ, Mo Y, Thomson MJ, Chin J, Delavina CB, Diaz GQ, Hernandez JE (2012) Mapping QTL for heat tolerance at flowering stage in rice using SNP markers. Plant Breed 131:33–41CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mitchell R. Lucas
    • 1
  • Jeffery D. Ehlers
    • 1
  • Bao-Lam Huynh
    • 2
  • Ndeye-Ndack Diop
    • 1
  • Philip A. Roberts
    • 2
  • Timothy J. Close
    • 1
  1. 1.Department of Botany and Plant SciencesUniversity of California RiversideRiversideUSA
  2. 2.Department of NematologyUniversity of California RiversideRiversideUSA

Personalised recommendations