Markers for breeding heat-tolerant cowpea
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The warm-season legume, cowpea (Vigna unguiculata), is an important crop that performs well in marginal environments. The effects of high temperature are among the most substantial challenges faced by growers of cowpea. Heat injury during late reproductive development sterilizes pollen such that no fruit is set. To study the inheritance of this trait and to deliver resources to breed cowpea with enhanced tolerance to heat, we performed a quantitative trait locus (QTL) analysis using 141 individuals from a recombinant inbred population made from a cross between cowpea varieties CB27 and IT82E-18. Five regions, which represent 9 % of the cowpea genome, explain 11.5–18.1 % of the phenotypic variation and are tagged with 48 transcript-derived single nucleotide polymorphism markers. Favorable haplotypes were donated by CB27 for four of these regions while IT82E-18 was the source of tolerance explained by the fifth QTL. Homeologous regions in soybean contain several genes important for tolerance to heat, including heat shock proteins, heat shock transcription factors, and proline transporters. This work presents essential information for marker-assisted breeding and supports previous findings concerning heat-induced male sterility in cowpea.
KeywordsLegume Heat QTL analysis Haplotype Synteny Breeding
This work was supported by the Generation Challenge Program (GCP) through a grant from the Bill and Melinda Gates Foundation and US Agency for International Development Collaborative Research Support Program GDG-G-00-02-00012-00 and EDH-A-00-07-00005. We acknowledge the collaborative effort led by members of the GCP, the Centro International de Mejoramiento de Maiz y Trigo within the Integrated Breeding Platform, and members of the University of California Riverside cowpea team for the development of the cowpea Kbiosciences genotyping platform and for the implementation of Genotyping Support Services which can be accessed by following the link: http://www.generationcp.org/gss_homepage.
- Hall AE (1992) Breeding for heat tolerance. Plant Breed Rev 10:129–168Google Scholar
- Mason RE, Mondal S, Beecher FW, Pacheco A, Jampala B, Ibrahim AMH, Hays DB (2010) QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica 174:423–436Google Scholar
- Lamesch P, Berardini TZ, Li D, Swarbreck D, Wilks C, Sasidharan R, Muller R, Dreher K, Alexander DL, Garcia-Hernandez M, Karthikeyan AS, Lee CH, Nelson WD, Ploetz L, Singh S, Wensel A, Huala E (2011) The Arabidopsis information resource (TAIR): improved gene annotation and new tools. Nucleic Acids Res 40:1202–1210CrossRefGoogle Scholar
- Lane A, Jarvis A (2007) Changes in climate will modify the geography of crop suitability: agricultural biodiversity can help with adaptation. J SAT Agric Res 4:1–12Google Scholar
- Medicago truncatula HapMap Project (2010) Mt3.5 genome assembly annotation November 2010, Nevin Young, University of Minnesota, St. Paul, MN. Available at http://medicagohamap.org/
- Muchero W, Diop NN, Bhat PR, Fenton RD, Wanamaker S, Pottorff M, Hearne S, Cisse N, Fatokun C, Ehlers JD, Roberts PA, Close TJ (2009) A consensus genetic map of cowpea [Vigna unguiculata (L) Walp.] and synteny based on EST-derived SNPs. Proc Natl Acad Sci USA 106(43):18159–18164PubMedCrossRefGoogle Scholar
- Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, GRimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183PubMedCrossRefGoogle Scholar
- Van Ooijen JW (2004) MapQTL5. Software for the mapping of quantitative trait loci in experimental populations, Kyazma BV, Wageningen, NetherlandsGoogle Scholar
- Wanamaker S and Close TJ (2011) HarvEST. HarvEST:Cowpea version 1.27, University of California Riverside. Available at http://harvest.ucr.edu