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Association mapping for soilborne pathogen resistance in synthetic hexaploid wheat

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Abstract

Soilborne pathogens such as cereal cyst nematode (CCN; Heterodera avenae) and root lesion nematode (Pratylenchus neglectus; PN) cause substantial yield losses in the major cereal-growing regions of the world. Incorporating resistance into wheat cultivars and breeding lines is considered the most cost-effective control measure for reducing nematode populations. To identify loci with molecular markers linked to genes conferring resistance to these pathogens, we employed a genome-wide association approach in which 332 synthetic hexaploid wheat lines previously screened for resistance to CCN and PN were genotyped with 660 Diversity Arrays Technology (DArT) markers. Two sequence-tagged site markers reportedly linked to genes known to confer resistance to CCN were also included in the analysis. Using the mixed linear model corrected for population structure and familial relatedness (Q+K matrices), we were able to confirm previously reported quantitative trait loci (QTL) for resistance to CCN and PN in bi-parental crosses. In addition, we identified other significant markers located in chromosome regions where no CCN and PN resistance genes have been reported. Seventeen DArT marker loci were found to be significantly associated with CCN and twelve to PN resistance. The novel QTL on chromosomes 1D, 4D, 5B, 5D and 7D for resistance to CCN and 4A, 5B and 7B for resistance to PN are suggested to represent new sources of genes which could be deployed in further wheat improvement against these two important root diseases of wheat.

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Acknowledgments

We thank all our colleagues from the Australian wheat community for their collaborative spirit and their willingness to share the data consistent with the aims of the synthetic evaluation project. We thank Grain Research and Development Corporation (GRDC), Australia, Department of Primary Industries, Horsham, Victoria and ICARDA, Syria for their financial support. The technical assistance of Jayne Wilson, DPI, Victoria and Professor Rudi Appels for critical review and editorial assistance with the manuscript are gratefully acknowledged.

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  1. Muhammad A. Mulki

    Present address: Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany

Authors and Affiliations

  1. International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria

    Muhammad A. Mulki, Abdulqader Jighly & Francis C. Ogbonnaya

  2. International Rice Research Institute (IRRI), Los Banos, Laguna, Philippines

    Gouyou Ye

  3. EH Graham Centre for Agricultural Innovation, Pine Gully Road, WaggaWagga, NSW, 2650, Australia

    Livinus C. Emebiri

  4. InterGrain Pty Ltd, 19 Ambitious Link, Bibra Lake, WA, 6163, Australia

    David Moody

  5. Grains Research and Development Corporation, P.O. Box 5367, Kingston, ACT, 2604, Australia

    Omid Ansari & Francis C. Ogbonnaya

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  1. Muhammad A. Mulki
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  2. Abdulqader Jighly
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Correspondence to Francis C. Ogbonnaya.

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Mulki, M.A., Jighly, A., Ye, G. et al. Association mapping for soilborne pathogen resistance in synthetic hexaploid wheat. Mol Breeding 31, 299–311 (2013). https://doi.org/10.1007/s11032-012-9790-z

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