Molecular validation of a multiple-allele recessive genic male sterility locus (BnRf) in Brassica napus L.
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The recessive genic male sterility (RGMS) line 9012AB has been used successfully for rapeseed hybrid production in China. This male sterility was previously thought to be controlled by three independent genes (Bnms3, Bnms4, and BnRf). Here, we initially attempted to locate the BnMs4 locus and develop feasible molecular markers for application in practical rapeseed breeding. However, we found that three sequence characterized amplified region markers and five simple sequence repeat markers identified as linked to BnMs4 were also genetically associated with BnRf, suggesting the possible co-localization of these two loci. Moreover, we proved that four intron-based polymorphism markers tightly linked or co-segregated with BnRf could also be mapped to BnMs4 with a genetic distance ranging from 0.054 to 0.594 cM. Finally, integration of genetic maps around BnRf and BnMs4 allows for the physical restriction of both loci to a DNA fragment of about 50 kb. Systematic genetic tests also provided evidence that the candidate BnMs4 locus was allelic to the BnRf locus. These results confirmed a major modification of the sterility inheritance model in 9012A: specifically, that this male sterility was essentially controlled by two loci (BnMs3 and BnRf), whereas the previously designated BnMs4 locus (hereafter designated as BnRf a ) was just one allele of BnRf in addition to BnRf b (the allele from 9012A) and BnRf c (the allele from temporary maintainer), with a dominance relationship of BnRf a > BnRf b > BnRf c . This inheritance model will simplify the breeding process involved with this RGMS line, especially with the BnRf allele-specific molecular markers identified here.
KeywordsBrassica napus Recessive genic male sterility Multiple-allele locus Molecular validation Marker-assisted selection
This research was funded by the Natural Science Foundation of China (31070279) and the National “863” Project (No. 2011AA10A104).
- Chen FX, Hu BC, Li QS (1993) Discovery and study of genic male sterility (GMS) material 9012A in Brassica napus L. (in Chinese). Acta Agric Univ Pekinensis 19(Suppl):57–61Google Scholar
- Chen FX, Hu BC, Li C, Li QS, Zhang ML (1995) Success in the breeding of all maintainer for recessive genic male sterile in Brassica napus L. (in Chinese). Acta Agron Sin 28:94–96Google Scholar
- Chen FX, Hu BC, Li C, Li QS, Chen WS, Zhang ML (1998) Genetic studies on GMS in Brassica napus L.: I. Inheritance of recessive GMS line 9012A (in Chinese). Acta Agron Sin 24:431–438Google Scholar
- Choi SR, Teakle GR, Plaha P, Kim JH, Allender CJ, Beynon E, Piao ZY, Soengas P, Han TH, King GJ, Barker GC, Hand P, Lydiate DJ, Batley J, Edwards D, Koo DH, Bang JW, Park BS, Lim YP (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theor Appl Genet 115:777–792PubMedCrossRefGoogle Scholar
- Dong FM, Hong DF, Liu PW, Xie YZ, He QB, Yang GS (2010) A novel genetic model for the recessive genic male sterility line 9012AB in rapeseed (Brassica napus L.) (in Chinese). J Huazhong Agric Univ 29:262–267Google Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Fu TD (2000) Breeding and utilization of rapeseed hybrid (in Chinese). Scientific and technical documents publishing house of Hubei, Wuhan. ISBN7-5352-1652-8Google Scholar
- Fu TD, Tu JX (2002) Present situation and prospects on the research and utilization of hybrid rapeseed (in Chinese). In: Liu HL (ed) Analects of crop breeding. China Agricultural University Press, Beijing, pp 235–250Google Scholar
- Hou GZ (2009) the research and utilization of recessive genic male sterility (RGMS) in Brassica napus L. (in Chinese). Scientific and technical documents publishing house. ISBN978-7-5023-6269-0Google Scholar
- Hou GZ, Wang H, Zhang RM (1990) Genetic study on genic male sterility (GMS) material no. 117A in Brassica napus L. (in Chinese). Oil Crop China 2:7–10Google Scholar
- Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with Mapmaker/Exp 3.0. Whitehead Institute Technical Report, CambridgeGoogle Scholar
- Liu J, Hong DF, Lu W, Liu PW, He QB, Yang GS (2008) Genetic analysis and molecular mapping of gene associated with dominant genic male sterility in rapeseed (Brassica napus L.). Genes Genomics 30:523–532Google Scholar
- Liu RH, Meng JL (2003) Mapdraw, a Microsoft Excel macro for draw genetic linkage maps based on given genetic linkage data. Hereditas (Beijing) 25:317–321Google Scholar
- Pan T, Zeng FY, Wu SH, Zhao Y (1988) A study on breeding and application GMS line of low eruci acid in rapeseed (B. napus). Oil Crop China 3:5–8Google Scholar
- Stiewe G, Pleines S, Coque M, Gielen J (2009) New hybrid system for Brassica napus. Euro Patent Appl EP2016821Google Scholar
- Wang L, Wei P, Liu Zh, Li C, Wang Y, Ji R, Feng H (2010) SSR mapping of the Msf, a multiple-allele male-fertility restorer gene in Chinese cabbage (Brassica campestris L. ssp. Pekinensis) (in Chinese). Acta Hort Sin 37:923–930Google Scholar
- Xia S, Cheng L, Zu F, Dun X, Zhou Z, Yi B, Wen J, Ma C, Shen J, Tu J, Fu T (2012) Mapping of BnMs4 and BnRf to a common microsyntenic region of Arabidopsis thaliana chromosome 3 using intron polymorphism markers. Theor Appl Genet. doi: 10.1007/s00122-011-1779-1
- Zu F, Xia SQ, Dun XL, Zhou ZF, Zeng FQ, Yi B, Wen J, Ma CZ, Shen JX, Tu JX, Fu TD (2010) Analysis of genetic model of a recessive genic male sterile line 7-7365AB in Brassica napus L. based on molecular markers. Sci Agric Sin 43:3067–3075Google Scholar