Molecular Breeding

, Volume 28, Issue 4, pp 623–634 | Cite as

Introgression of group 4 and 7 chromosomes of Ae. peregrina in wheat enhances grain iron and zinc density

  • Kumari Neelam
  • Nidhi Rawat
  • Vijay K. Tiwari
  • Sundip Kumar
  • Parveen Chhuneja
  • Kuldeep Singh
  • Gursharn S. Randhawa
  • Harcharan S. Dhaliwal


Dietary deficiency of iron and zinc micronutrients affects more than two billion people worldwide. Breeding for micronutrient-dense crops is the most sustainable and cost-effective approach for alleviation of micronutrient malnutrition. Three accessions of Aegilops peregrina (Hack.) Maire & Weill (2n = 28, UPUPSPSP), selected for high grain iron and zinc concentration were crossed with Triticum aestivum L. cv. Chinese Spring (Ph I ). The sterile F1 hybrids were backcrossed with elite wheat cultivars to get fertile BC2F2 derivatives. Some of the fertile BC2F2 derivatives showed nearly 100% increase in grain iron and more than 200% increase in grain zinc concentration compared to the recipient wheat cultivars. The development of derivatives with significantly higher grain micronutrients, high thousand-grain weight and harvest index suggests that the enhanced micronutrient concentration is due to the distinct genetic system of Ae. peregrina and not to the concentration effect. Genomic in situ hybridization, comparison of introgressed chromosomes with the standard karyotype of Ae. peregrina and simple sequence repeat marker analysis revealed the introgression of 7SP chromosomes in five selected derivatives, 7UP in four, group 4 and 4SP in three and a translocated 5UP of Ae. peregrina in one of the selected derivatives. Molecular marker analysis using the introgressed chromosome markers indicated that two of the BC2F3 progenies were stabilized as disomic addition lines. It could, therefore, be concluded that the group 4 and 7 chromosomes of Ae. peregrina carry the genes for high grain iron and zinc concentration.


Iron Zinc Wheat Ae. peregrina Introgression SSR markers GISH 



The contribution of the Department of Biotechnology, Ministry of Science and Technology, Government of India for supporting the research through a Network Project “Biofortification of wheat for enhanced iron and zinc content by conventional and molecular breeding” is acknowledged with thanks. The authors are grateful to the Head, Institute Instrumentation Centre, I.I.T., Roorkee, and Mr. R. Juyal for their help in micronutrient analysis.

Supplementary material

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Supplementary material 1 (PDF 13 kb)
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Supplementary material 2 (PDF 79 kb)
11032_2010_9514_MOESM3_ESM.pdf (395 kb)
Supplementary material 3 (PDF 395 kb)


  1. Aghaee-Sarbarzeh M, Ferrahi M, Singh S, Singh H, Friebe B, Gill BS, Dhaliwal HS (2002) Ph I -induced transfer of leaf and stripe rust-resistance genes from Aegilops triuncialis and Ae. geniculata to bread wheat. Euphytica 127:377–382CrossRefGoogle Scholar
  2. Badaeva ED, Amosova AV, Samatadze TE, Zoshchuk SA, Shostak NG, Chikida NN, Zelenin AV, Raupp WJ, Friebe B, Gill BS (2004) Genome differentiation in Aegilops. 4. Evolution of the U-genome cluster. Plant Syst Evol 246:45–76CrossRefGoogle Scholar
  3. Bouis HE (2007) The potential of genetically modified food crops to improve human nutrition in developing countries. J Dev Stud 43:79–96CrossRefGoogle Scholar
  4. Bouis HE, Welch RM (2010) Biofortification—a sustainable agricultural strategy for reducing micronutrient malnutrition in the global south. Crop Sci 50:20–32CrossRefGoogle Scholar
  5. Cakmak I, Pfeiffer WH, McClafferty B (2010) Biofortification of durum wheat with zinc and iron. Cereal Chem 87:10–20CrossRefGoogle Scholar
  6. Calderini DF, Monasterio I (2003) Are synthetic hexaploids a means of increasing grain element concentrations in wheat? Euphytica 134:169–178CrossRefGoogle Scholar
  7. Chen Q, Tsujimoto H, Gill BS (1994) Transfer of Ph I gene promoting homeologous pairing from Triticum speltoides into common wheat and their utilization in alien genetic introgression. Theor Appl Genet 88:97–101Google Scholar
  8. Chhuneja P, Dhaliwal HS, Bains NS, Singh K (2006) Aegilops kotschyi and Aegilops tauschii as sources for higher levels of grain iron and zinc. Plant Breed 125:529–531CrossRefGoogle Scholar
  9. Chhuneja P, Kaur S, Goel RK, Aghaee-Sarbarzeh M, Prashar M, Dhaliwal HS (2008) Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk to bread wheat (Triticum aestivum L.). Genet Resour Crop Evol 55:849–859CrossRefGoogle Scholar
  10. Dhaliwal HS, Singh H, William M (2002) Transfer of rust resistance from Aegilops ovata into bread wheat (Triticum aestivum L.) and molecular characterisation of resistant derivatives. Euphytica 126:153–159CrossRefGoogle Scholar
  11. Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996a) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87CrossRefGoogle Scholar
  12. Friebe B, Tuleen NA, Badaeva ED, Gill BS (1996b) Cytogenetic identification of Triticum peregrinum chromosomes added to wheat. Genome 39:272–276PubMedCrossRefGoogle Scholar
  13. Friebe B, Tuleen NA, Gill BS (1999) Development and identification of a complete set of Triticum aestivum-Aegilops geniculata chromosome addition lines. Genome 42:374–380Google Scholar
  14. Garg M, Tanaka H, Ishikawa N, Takaka K, Yanaka H, Tsujimoto H (2009) Agropyron elongatum HMW glutenins have a potential to improve wheat end product quality through targeted chromosome introgression. J Cereal Sci 50:358–363CrossRefGoogle Scholar
  15. Jauhar PP (2008) Synthesis of an FBH-resistant durum disomic alien addition line with a pair of diploid wheat grass chromosomes. Cereal Res Commun 36:77–82CrossRefGoogle Scholar
  16. Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007a) Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet 114:1379–1389PubMedCrossRefGoogle Scholar
  17. Kuraparthy V, Sood S, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007b) A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58. Crop Sci 47:1–9 Google Scholar
  18. Monasterio I, Graham RD (2000) Breeding for trace minerals in wheat. Food Nutr Bull 21:392–396Google Scholar
  19. Murray MG, Thomson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 8:4321–4325PubMedCrossRefGoogle Scholar
  20. Oliver RE, Cai X, Xu SS, Chen X, Stack RW (2005) Wheat-alien species derivatives: a novel source of resistance to Fusarium head blight in wheat. Crop Sci 45:1353–1360CrossRefGoogle Scholar
  21. Pedersen C, Rasmussen SK, Linde-Laursen I (1996) Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence. Genome 39:93–104Google Scholar
  22. Peleg Z, Saranga Y, Yazici A, Fahima T, Ozturk L, Cakmak I (2008) Grain zinc, iron and protein concentrations and zinc-efficiency in wild emmer wheat under contrasting irrigation regimes. Plant Soil 306:57–67CrossRefGoogle Scholar
  23. Pestsova E, Ganal MW, Röder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697PubMedCrossRefGoogle Scholar
  24. Rayburn AL, Gill BS (1987) Molecular analysis of the D-genome of the Triticeae. Theor Appl Genet 73:385–388Google Scholar
  25. Rawat N, Tiwari VK, Neelam K, Randhawa GS, Singh K, Chhuneja P, Dhaliwal HS (2009a) Development and characterization of wheat- Aegilops kotschyi amphiploids with high grain iron and zinc. Plant Genet Resour 7:271–280CrossRefGoogle Scholar
  26. Rawat N, Tiwari VK, Singh N, Randhawa GS, Singh K, Chhuneja P, Dhaliwal HS (2009b) Evaluation and utilization of Aegilops and wild Triticum species for enhancing iron and zinc content in wheat. Genet Res Crop Evol 56:53–64CrossRefGoogle Scholar
  27. Röder MS, Korzun V, Wandehake K, Planschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  28. Shi R, Li H, Tong Y, Jing R, Zhang F, Zou C (2008) Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain. Plant Soil 306:95–104CrossRefGoogle Scholar
  29. Singh NK, Raghuvanshi S, Srivastava SK, Gaur A, Pal AK, Dalal V, Ghazi IA, Bhargav A, Yadav M, Dxit A, Batra K, Gaikwad K, Sharma TR, Mohanty A, Bharti AK, Kapur A, Gupta V, Kumar D, Vij S, Vidianathan R, Khurana P, Sharma S, McCombie WR, Messing J, Wing K, Sasaki T, Khurana P, Mohapatra Trilochan, Khurana JP, Tyagi AK (2004) Sequencec analysis of the long arm of rice chromosome 11 for rice- wheat synteny. Funct Integr Genomics 4:102–114PubMedCrossRefGoogle Scholar
  30. Somers DJ, Peter I, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  31. Stangoulis JCR, Huynh B, Welch RM, Choi E, Graham RD (2007) Quantitative trait loci for phytate in rice grain and their relationship with grain micronutrient content. Euphytica 154:289–294CrossRefGoogle Scholar
  32. Tiwari VK, Rawat N, Neelam K, Randhawa GS, Singh K, Chhuneja P, Dhaliwal HS (2008) Development of Triticum turgidum ssp. durumAegilops longissima amphiploids with high iron and zinc content through unreduced gamete formation in F1 hybrids. Genome 51:757–766PubMedCrossRefGoogle Scholar
  33. Tiwari VK, Rawat N, Chhuneja P, Neelam K, Aggarwal R, Randhawa GS, Dhaliwal HS, Keller B, Singh K (2009) Mapping of quantitative trait loci for grain iron and zinc concentration in A genome diploid wheat. J Hered 100:771–776PubMedCrossRefGoogle Scholar
  34. Tiwari VK, Rawat N, Neelam K, Malik S, Randhawa GS, Dhaliwal HS (2010) Substitution of 2S and 7U chromosomes of Aegilops kotschyi in wheat enhances grain iron and zinc concentration. Theor Appl Genet 121:259–269PubMedCrossRefGoogle Scholar
  35. Zhang P, Friebe B, Lukaszewski AJ, Gill BS (2001) The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosoma 110:335–344Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Kumari Neelam
    • 1
  • Nidhi Rawat
    • 1
  • Vijay K. Tiwari
    • 1
  • Sundip Kumar
    • 2
  • Parveen Chhuneja
    • 3
  • Kuldeep Singh
    • 3
  • Gursharn S. Randhawa
    • 1
  • Harcharan S. Dhaliwal
    • 1
  1. 1.Department of BiotechnologyIndian Institute of Technology RoorkeeRoorkeeIndia
  2. 2.Department of Molecular Biology and Genetic EngineeringCBSH, GB Pant University of Agriculture and TechnologyPantnagarIndia
  3. 3.School of Agricultural BiotechnologyPunjab Agricultural UniversityLudhianaIndia

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