Fine mapping of a male sterility gene, vr1, on chromosome 4 in rice
xs1 is a male sterile rice mutant derived from a spontaneous mutation. Pollen development in the xs1 mutant proceeds normally until the vacuolation stage, at which time xs1 pollen fails to vacuolate and no viable pollen is produced. Genetic analysis indicates that the xs1 mutant phenotype is controlled by a single recessive gene, designated vacuolation retardation 1 (vr1), which was mapped to rice chromosome 4. In order to fine-map the vr1 locus, two large mapping populations were generated and several SSR and InDel markers were developed from publicly available rice genomic sequences. By employing a strategy of chromosome-walking, the vr1 gene was finally located within a genetic interval of 0.27 cM, flanked by the markers FID30 and FS15, with distances of 0.11 and 0.16 cM, respectively, and co-segregating with the marker FC4-2. Based on the japonica rice genome sequence, the vr1 locus is estimated to cover a 48-kb region containing eight putative genes. Our results will facilitate the cloning and functional characterization of the vr1 gene.
KeywordsMale sterility Molecular maker Fine mapping Rice
The work is financially supported by the National Natural Science Foundation of China (30800084), the Hi-Tech Research and Development Program (863) of China (2006AA10A103), and the National Basic Research (973) Program of China (2006CB101700).
- Crouzet J, Levy-Schil S, Cameron B, Cauchois L, Rigault S, Rouyez MC, Blanche F, Debussche L, Thibaut D (1991) Nucleotide sequence and genetic analysis of a 13. 1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase. J Bacteriol 173:6074–6087PubMedGoogle Scholar
- Feng JH, Lu YG, Liu XD, Xu XB (2001) Pollen development and its stage in rice. Chin J Rice Sci 15(1):21–28Google Scholar
- Lander ES, Green P, Abrahamson J (1987) Mapmarker: an interactive computer for constructing primary genetics linkage maps of experimental and natural populations. Genetics 1:174–181Google Scholar
- Li SC, Yang L, Deng QM, Wang SQ, Wu FQ, Li P (2006a) Phenotypic characterization of a female sterile mutant in rice. J Integr Plant Biol 48:308–314Google Scholar
- Miao Y, Chen MC (2000) Studies on molecular mechanism of male sterility in plants. Chin Bull Bot 17:1–10Google Scholar
- Moritoh S, Miki D, Akiyama M, Kawahara M, Izawa T, Maki H, Shimamoto K (2005) RNAi-mediated silencing of OsGEN-L(OsGEN-like)), a new member of the rad2/xpg nuclease family, causes male sterility by defect of microspore development in rice. Plant Cell Physiol 46(5):699–715PubMedCrossRefGoogle Scholar
- Nonomura K, Nakano M, Fukuda T, Eiguchi M, Miyao A, Miyao A, Hirochika H, Kurata N (2004a) The novel gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of rice encodes a putative coiled-coil protein required for homologous chromosome pairing in meiosis. Plant Cell 16:1008–1020PubMedCrossRefGoogle Scholar
- Tan HX, Wen TQ, Zhang DB (2007) Molecular mechanisms of pollen development in Oryza sativa. Chin Bull Bot 24(3):330–339Google Scholar
- Zhu YG, Yang DC (1992) Studies and utilization on photoperiod-sensitive genetic male sterile in rice. Wuhan University Press, ChinaGoogle Scholar