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Molecular Breeding

, Volume 27, Issue 4, pp 525–532 | Cite as

Utility testing of an apple skin color MdMYB1 marker in two progenies

  • Yanmin Zhu
  • Kate Evans
  • Cameron Peace
Article

Abstract

A reported allele-specific dCAPS (derived cleaved amplified polymorphic sequence) marker, within the gene for the anthocyanin regulatory transcription factor MdMYB1, associated with apple fruit skin color, was tested in 17 elite breeding parents and two apple seedling progenies. In both progenies, the red skin color phenotype was usually associated with the MdMYB1-1 allele. This dCAPS marker provided approximately 80% predictability in a ‘Golden Delicious’ × ‘Arlet’ and a ‘Honeycrisp’ × ‘Cripps Pink’ progeny. Other potential genetic co-regulators may explain the less-than-perfect association. The specific dCAPS bands associated with red skin for the latter population were not the same as those identified in the former population or those reported in previous studies, indicating that skin color genotyping based on this marker will require prior association between specific marker alleles and color phenotypes for any given cross. The current form of this marker could be a useful tool for apple marker-assisted breeding, particularly in breeding programs in which ‘Golden Delicious’ is a parent.

Keywords

dCAPS Functional marker Malus × domestica Marker-assisted breeding MYB transcription factor 

Notes

Acknowledgments

The authors are grateful to Fred Bliss, James McFerson, and Tianbao Yang for their helpful comments. We wish to thank Mallela Magana and Bonnie Konishi for their contribution in tissue collection, DNA extraction, and PCR amplification. This study was supported by Washington Tree Fruit Research Commission.

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Copyright information

© US Government 2010

Authors and Affiliations

  1. 1.USDA, ARS, Tree Fruit Research LabWenatcheeUSA
  2. 2.Tree Fruit Research and Extension CenterWashington State UniversityWenatcheeUSA
  3. 3.Department of Horticulture and Landscape ArchitectureWashington State UniversityPullmanUSA

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