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Mapping of southern corn rust-resistant genes in the W2D inbred line of maize (Zea mays L.)

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Abstract

Southern corn rust, caused by Puccinia polysora Underw., has destructive potential on the susceptible host. In this study, the resistance inheritance was investigated in an F 2 and its F 2:3 populations derived from a cross from two inbred lines W2D (resistant) and W222 (susceptible). The 3:1 ratio of resistant to susceptible plants indicated that the resistance is controlled by one dominant gene (named as RppD). The gene RppD was located by means of the F 2 population. Total of 11 markers, including five SSR markers, five sequence-tagged site markers and one cleaved-amplified polymorphic sequence (CAPS) marker, were identified to narrow the gene RppD down to a smaller interval. The closest markers flanking RppD were SSR marker umc1291 and CAPS marker CAPS858, with genetic distances of 2.9 and 0.8 cM, respectively. Moreover, RppD might be a novel Rpp resistance gene or haplotype differing from RppQ and RppP25 according to an allelism test among the three crosses W2D × Qi319, W2D × P25 and Qi319 × P25. As a result, RppD haplotype might be helpful to maize germplasm enhancement and disease-resistant breeding.

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Acknowledgments

This work was supported by grants from the Chinese National Programs for High Technology Research and Development (863 Program) (No. 2007AA10Z172), the National Science and Technology Sustained Project of China (973 Project) (No. 2006BAD13B03) and the National Natural Science Foundation of China (NO. 30370885).

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Authors and Affiliations

  1. National Maize Improvement Center of China, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, No. 2 West Yuanmingyuan Road, 100193, Haidian District, Beijing, China

    Ya Zhang, Li Xu, Deng-Feng Zhang, Jing-Rui Dai & Shou-Cai Wang

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  1. Ya Zhang
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  2. Li Xu
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  3. Deng-Feng Zhang
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  4. Jing-Rui Dai
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  5. Shou-Cai Wang
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Correspondence to Shou-Cai Wang.

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Zhang, Y., Xu, L., Zhang, DF. et al. Mapping of southern corn rust-resistant genes in the W2D inbred line of maize (Zea mays L.). Mol Breeding 25, 433–439 (2010). https://doi.org/10.1007/s11032-009-9342-3

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  • DOI: https://doi.org/10.1007/s11032-009-9342-3

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