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Identification of two major resistance genes against race IE-1k of Magnaporthe oryzae in the indica rice cultivar Zhe733

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Abstract

The race IE-1k of Magnaporthe oryzae recovered from the Southern US overcomes the resistance (R) gene Pita. The objectives of the present study were to identify and tag R genes to IE-1k for rice breeding. TM2, S1, 94071, and B isolates of the race IE-1k were used to identify and map R genes from a resistant indica rice cultivar Zhe733 using a recombinant inbred line population from a cross of the genetic stock KBNTlpa1-1 and Zhe733. The ratio of 3 resistant:1 susceptible in 162 RIL of an F10-11 KBNTlpa1-1/Zhe733 (K/Z) population indicated that two major R genes in Zhe733 confer resistance to IE-1k. A total of 118 polymorphic simple sequence repeat markers were analyzed in 162 F10-11 individuals of the K/Z population to determine chromosomal locations of the loci conferring resistance to race IE-1k using composite interval mapping. Two major R genes temporarily designated as Pi42(t) and Pi43(t) each providing complete resistance to IE-1k were identified on chromosomes 8 and 11, respectively. RILs containing Pi42(t) and Pi43(t) were also resistant to other US races IB-1, IB-45, IB-49, IB-54, IC-17, IE-1, IG-1, and IH-1. The Pi42(t) gene was mapped between RM310 and RM72, and the location of Pi43(t) was closely associated with two flanking SSR markers RM1233 and RM224 on chromosome 11 in a chromosomal region carrying the resistance gene Pi1. Two molecular markers RM72 and RM1233 identified in this study should be useful for fine mapping and for facilitating incorporation of Pi42(t) and Pi43(t) into advanced breeding lines by marker-assisted selection.

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Acknowledgments

The authors thank the Arkansas Rice Research and Promotion Board for partial financial support, Michael Lin, Tony Beaty, Kristen Pratt, Drs. Joseph Kepiro, Hesham Agrama, Robert Fjellstrom (USDA-ARS DB NRRC) for excellent technical support, Lorie Bernhardt and Dr. J. Neil Rutger of Genetic stock-Oryza (GSOR) collection of DB NRRC for providing the RIL population of the cross of KBNTlpa1-1 and Zhe733, and Dr. Thomas Mitchell (Department of Plant Pathology, Ohio State University) and Dr. Stephen Neate (Department of Plant Pathology, North Dakota State University) for technical comments. This report is part of the NP301 project # 6225-21220-002-00 of USDA-ARS and is also supported by National Science Foundation under Grant No. 0638820, USA.

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Authors and Affiliations

  1. Rice Research and Extension Center, University of Arkansas, 72160, Stuttgart, AR, USA

    S. Lee, Y. Wamishe & G. Liu

  2. USDA-ARS Dale Bumpers National Rice Research Center (USDA-ARS DB NRRC), 72160, Stuttgart, AR, USA

    Y. Jia & M. H. Jia

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  1. S. Lee
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  2. Y. Wamishe
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  3. Y. Jia
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  4. G. Liu
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  5. M. H. Jia
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Correspondence to Y. Jia.

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The authors S. Lee and Y. Wamishe contribute equally to this work.

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Lee, S., Wamishe, Y., Jia, Y. et al. Identification of two major resistance genes against race IE-1k of Magnaporthe oryzae in the indica rice cultivar Zhe733. Mol Breeding 24, 127–134 (2009). https://doi.org/10.1007/s11032-009-9276-9

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  • DOI: https://doi.org/10.1007/s11032-009-9276-9

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