The integration of mutant loci affecting maize endosperm development in a dense genetic map using an AFLP-based procedure
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In this paper, 10 mutations conditioning the appearance of defective, miniature or collapsed endosperm, but with normal sporophyte development, were considered. Homozygous mutant kernels have reduced grain weight, kernel size, density and, in some of these, higher than normal seed protein content. The mutant loci were integrated into a high-resolution genetic map in order to associate them to specific genes. We have placed 1167 AFLP markers on a consensus map using IBM2 as a backbone and reaching an average of 1 marker every 1.9 cM. We have identified AFLP markers linked to all individual mutant alleles. BSA was adopted to screen the largest possible number of primer combinations on homozygous F3 mutant and wild type plants. The ten mutant loci are linked to the closest AFLP or SSR markers with distances ranging from 0 to 17.9 cM. The genes we have defined by the existence of mendelian mutants can now be considered good candidates for testing the association to QT loci.
KeywordsZea mays Viable endosperm mutants Bulked segregant analysis Integrated molecular maps
We gratefully acknowledge support from Sieglinde Effgen and Hakan Özkan for AFLP analysis. This work was supported by the Ministero dell’Università e della Ricerca of Italy (FIRB contract no. RBAU01MHMR, Functionmap and PRIN contract no. 2006074848).
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