Advertisement

Molecular Breeding

, Volume 22, Issue 4, pp 517–526 | Cite as

Detection of Fusarium head blight resistance QTLs using five populations of top-cross progeny derived from two-row × two-row crosses in barley

  • Kazuhiro Sato
  • Kiyosumi Hori
  • Kazuyoshi Takeda
Article

Abstract

Fusarium head blight (FHB) resistance was evaluated in five recombinant inbred (RI) populations. The RI populations consisted of top-cross progeny derived from a diallel set of crosses. Each of five two-row barley lines differing in response to FHB were crossed with ‘Harbin 2-row’. FHB severity was scored on an 11-point scale, where resistant = 0 and susceptible = 10, based on the ‘cut-spike test’. Disease data were obtained for each population for 2 or 3 years. Linkage maps comprised of expressed sequence tag (EST) markers were developed for each population and used for quantitative trait locus (QTL) detection. Thirty two QTLs were detected using all data sets (individual populations and years). Thirteen QTLs were detected using averages across years; 10 of these were consistent across the individual year and average data sets. These QTLs clustered at 14 regions, with clusters on all chromosomes. At 11 of these clusters, Harbin 2-row contributed FHB resistance alleles. No QTLs were detected near the row type (vrs1) locus in any of the five RI populations, suggesting that the FHB resistance QTL in this region reported in two-row × six-row crosses may be pleiotropic effect of vrs1. QTL were coincident with the flowering type locus (cly1/Cly2) on chromosome 2H in every population. Some QTL × QTL interactions were significant, but these were smaller than QTL main effects. Considering the pleiotropic effect of spike morphology on FHB resistance, future FHB resistance mapping efforts in barley should focus on cross combinations in which alleles at vrs1 are not segregating.

Keywords

Barley Fusarium head blight Hordeum vulgare L. Scab Quantitative trait loci 

Abbreviations

FHB

Fusarium head blight

RI

Recombinant inbred

EST

Expressed sequence tag

QTL

Quantitative trait locus

SNP

Single nucleotide polymorphism

AFLP

Amplified fragment length polymorphism

SSR

Simple sequence repeat

STS

Sequence tagged site

CAPS

Cleaved amplified polymorphic sequence

Notes

Acknowledgements

We would like to thank Dr. Patrick M. Hayes for his critical reading of the manuscript, Ms. N. Nankaku and Y. Motoi, Res. Inst. Biores., Okayama Univ., for their technical assistance.

Supplementary material

11032_2008_9195_MOESM1_ESM.xls (44 kb)
(XLS 45 kb)

References

  1. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766Google Scholar
  2. Dahleen LS, Agrama HA, Horsley RD, Steffenson BJ, Schwarz PB, Mesfin A, Franckowiak JD (2003) Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley. Theor Appl Genet 108:95–104PubMedCrossRefGoogle Scholar
  3. de la Pena RC, Smith KP, Capettini F, Muehlbauer GJ, Gallo-Meagher M, Dill-Macky R, Somers DA, Rasmusson DC (1999) Quantitative trait loci associated with resistance to Fusarium head blight and kernel discoloration in barley. Theor Appl Genet 99:561–569CrossRefGoogle Scholar
  4. Greene RA, DiMeo JJ, Malone ME, Swartwout S, Liu J, Buzby PR (2002) AcycloPrime, a novel method for SNP analysis using fluorescence polarization. Proc SPIE 4626:332–339CrossRefGoogle Scholar
  5. Hori K, Kobayashi T, Sato K, Takeda K (2005) QTL analysis of Fusarium head blight resistance using a high-density linkage map in barley. Theor Appl Genet 111:1661–1672PubMedCrossRefGoogle Scholar
  6. Hori K, Sato K, Kobayashi T, Takeda K (2006) QTL analysis of Fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties. Breed Sci 56:25–30CrossRefGoogle Scholar
  7. Horsley RD, Schmierer D, Maier C, Kudrna D, Urrea CA, Steffenson BJ, Schwarz PB, Franckowiak JD, Green MJ, Zhang B, Kleinhofs A (2006) Identification of QTLs associated with Fusarium head blight resistance in barley accession CIho 4196. Crop Sci 46:145–156CrossRefGoogle Scholar
  8. Kanatani R, Takeda K (1991) A method for mass sporulation in a scab disease pathogen (Fusarium graminearum Schwabe). Nogaku Kenkyu 62:177–189Google Scholar
  9. Komatsuda T, Pourkheirandish M, He C, Azhaguvel P, Kanamori H, Perovic D, Stein N, Graner A, Wicker T, Tagiri A, Lundqvist U, Fujimura T, Matsuoka M, Matsumoto T, Yano M (2007) Six-rowed barley originated from a mutation in a homeodomain-leucine zipper I-class homeobox gene. Proc Natl Acad Sci 104:1424–1429PubMedCrossRefGoogle Scholar
  10. Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175Google Scholar
  11. Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181PubMedCrossRefGoogle Scholar
  12. Ma Z, Steffenson BJ, Prom LK, Lapitan NL (2000) Mapping of quantitative trait loci for Fusarium head blight resistance in barley. Phytopathology 90:1079–1088CrossRefGoogle Scholar
  13. Mesfin A, Smith KP, Dill-Macky R, Evans CK, Waugh R, Gustus CD, Muehlbauer GJ (2003) Quantitative trait loci for Fusarium head blight resistance in barley detected in a two-rowed by six-rowed population. Crop Sci 43:307–318Google Scholar
  14. Ramsay L, Macaulay M, degli Ivanissevich S, MacLean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005PubMedGoogle Scholar
  15. Sato K, Nankaku N, Motoi Y, Takeda K (2004) Large scale mapping of ESTs on barley genome. In: Spunar J, Janikova J (eds) Proceedings of the 9th international barley genetics symposium, vol 1. Brno, Czech Republic, pp 79–85Google Scholar
  16. Steffenson BJ (2003) Fusarium head blight of barley: impact, epidemics, management, and strategies for identifying and utilizing genetic resistance. In: Leonard KJ, Bushnell WR (eds) Fusarium head blight of wheat and barley. American Phytopathology Press, St. Paul, pp 241–295Google Scholar
  17. Takeda K (1990) Selection response and parent-offspring correlation of the resistance to Fusarium head blight in barley. Jpn J Breed 40:91–101Google Scholar
  18. Takeda K, Heta H (1989) Establishing the testing method and a search for the resistant varieties to Fusarium head blight in barley. Jpn J Breed 39:203–216Google Scholar
  19. Takeda K, Wu J (1996) Inheritance of the resistance to Fusarium head blight in F1 hybrids of barley. Breed Sci 46:269–274Google Scholar
  20. Takeda K, Heta H, Fukuyama T (1986) A test of Fusarium blight resistance of barley by inoculation on cut-spike at anthesis. Nogaku Kenkyu 61:129–138Google Scholar
  21. Tanno K, Taketa S, Takeda K, Komatsuda T (2002) A DNA marker closely linked to the vrs1 locus (row-type gene) indicates multiple origins of six-rowed cultivated barley (Hordeum vulgare L.). Theor Appl Genet 104:54–60PubMedCrossRefGoogle Scholar
  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882CrossRefGoogle Scholar
  23. Turuspekov Y, Mano Y, Honda I, Kawada N, Watanabe Y, Komatsuda T (2004) Identification and mapping of cleistogamy genes in barley. Theor Appl Genet 109:480–487PubMedCrossRefGoogle Scholar
  24. Wang S, Basten CJ, Zeng ZB (2005) Windows QTL Cartographer version 2.5. Department of Statistics, North Carolina State University, Raleigh. http://statgen.ncsu.edu/qtlcart/HTML/index.html
  25. Yoshida M, Kawada N, Tohnooka T (2005) Effect of row type, flowering type and several other spike characters on resistance to Fusarium head blight in barley. Euphytica 141:217–227CrossRefGoogle Scholar
  26. Zhou X, Chao M, Liang X (1991) Screening and testing of barley varieties for scab resistance. Acta Phytophylacia Sin 18:261–264Google Scholar
  27. Zhu H, Gilchrist L, Hayes P, Kleinhofs A, Kudrna D, Liu Z, Prom L, Steffenson B, Toojinda T, Vivar H (1999) Does function follow form? Principal QTLs for Fusarium head blight (FHB) resistance are coincident QTLs for inflorescence traits and plant height in a doubled haploid population of barley. Theor Appl Genet 99:1221–1232CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kazuhiro Sato
    • 1
  • Kiyosumi Hori
    • 1
  • Kazuyoshi Takeda
    • 1
  1. 1.Research Institute for BioresourcesOkayama UniversityKurashikiJapan

Personalised recommendations