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QTL mapping of resistance to Fusarium ear rot using a RIL population in maize

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Abstract

Fusarium ear rot is a prevalent disease in maize, reducing grain yields and quality. Resistance breeding is an efficient way to minimize losses caused by the disease. In this study, 187 lines from a RIL population along with the resistant (87-1) and susceptible (Zong 3) parents were planted in Zhengzhou and Beijing with three replications in years 2004 and 2006. Each line was artificially inoculated using the nail-punch method. Significant genotypic variation in response to Fusarium ear rot was detected in both years. Based on a genetic map containing 246 polymorphic SSR markers with average genetic distances of 9.1 cM, the ear-rot resistance QTL were firstly analyzed by composite interval mapping (CIM). Three QTL were detected in both Zhengzhou and Beijing in 2004; and three and four QTL, respectively, were identified in 2006. The resistant parent contributed all resistance QTL. By using composite interval mapping and a mixed model (MCIM), significant epistatic effects on Fusarium ear rot as well as interactions between mapped loci and environments were observed across environments. Two QTL on chromosome 3 (3.04 bin) were consistently identified across all environments by the two methods. The major resistant QTL with the largest effect was flanked by markers umc1025 and umc1742 on chromosome 3 (3.04 bin), explaining 13–22% of the phenotypic variation. The SSR markers closely flanking the major resistance QTL will facilitate marker-assisted selection (MAS) of resistance to Fusarium ear rot in maize breeding programs.

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Acknowledgements

This work was supported by the Chinese High Technology Project. The authors acknowledge the help of Professor R. A. McIntosh for language editing.

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Authors and Affiliations

  1. National Maize Improvement Centre of China, China Agricultural University, Yuanmingyuan West Road, Haidian, 100094, Beijing, China

    Jun-Qiang Ding, Subhash Chander, Jian-Bing Yan & Jian-Sheng Li

  2. Crop Research Institute, Chinese Academy of Agricultural Sciences, Zhongguanchun South Street 12, Haidian, 10081, Beijing, China

    Xiao-Ming Wang

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  1. Jun-Qiang Ding
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  2. Xiao-Ming Wang
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  3. Subhash Chander
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  4. Jian-Bing Yan
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  5. Jian-Sheng Li
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Correspondence to Jian-Sheng Li.

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Ding, JQ., Wang, XM., Chander, S. et al. QTL mapping of resistance to Fusarium ear rot using a RIL population in maize. Mol Breeding 22, 395–403 (2008). https://doi.org/10.1007/s11032-008-9184-4

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  • DOI: https://doi.org/10.1007/s11032-008-9184-4

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