Molecular Breeding

, Volume 20, Issue 4, pp 359–374 | Cite as

Genetic mapping and localization of quantitative trait loci affecting fungal disease resistance and leaf morphology in grapevine (Vitis vinifera L)

  • Leocir J. Welter
  • Nilgün Göktürk-Baydar
  • Murat Akkurt
  • Erika Maul
  • Rudolf Eibach
  • Reinhard Töpfer
  • Eva M. Zyprian


The aim of this study was the improvement of a genetic map of the F1 population from the cross between the fungus-resistant grapevine cv. “Regent” and the susceptible cv. “Lemberger” and its use to localize factors affecting pathogen resistance and leaf morphology. To construct an integrated map combining the information from both parental meiotic recombination frequencies co-dominant microsatellite markers were employed. Resistance gene analog (RGA)-derived and sequence characterized amplified regions (SCAR) markers correlated with powdery and downy mildew resistance were additionally mapped. The new integrated map contains 398 markers aligned along 19 linkage groups, covers a total length of 1,631 cM and shows an average distance between markers of 4.67 cM. One hundred and twenty-two microsatellite markers were newly mapped. This genetic map was used to localize QTLs (quantitative trait loci) conferring resistance to powdery and downy mildew pathogens transmitted from “Regent”. Factors influencing specific leaf morphology traits were identified in addition. A major QTL for powdery mildew resistance and one major and one minor QTL for downy mildew resistance were detected. Some RGA-derived markers are found co-located in the region covered by the major QTL for resistance to downy mildew hinting at their putative functional relevance. Furthermore, 27 QTLs affecting leaf morphology descriptors were identified. This map is an important tool for grapevine breeding and resistance research.


Erysiphe (syn. Uncinula) necator Genetic mapping Leaf morphology Pathogen resistance Plasmopora viticola QTL analysis Resistance gene analogs 



We wish to thank Gabriele di Gaspero (University of Udine, Italy) for exchange of information prior to publication. Sabine Wiedemann-Merdinoglu (INRA Colmar) contributed with downy mildew resistance data from greenhouse experiments in order to validate them with field data. Johan W. Van Ooijen was helpful for QTL analysis. Charlotte Gleich provided expert technical assistance. This work was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES)-Brasilia/Brazil (Ph.D. fellowship provided to L.J.W.), the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD, visiting scientist fellowship for N. G.-B.), Ankara University (Ph.D fellowship provided to M. A.) and funds from the German Federal Ministry of Nutrition, Agriculture and Consumers Protection (Bundesministerium für Ernährung, Landwirtschaft und Verbraucherschutz, BMELV).


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Leocir J. Welter
    • 1
  • Nilgün Göktürk-Baydar
    • 1
    • 2
  • Murat Akkurt
    • 1
    • 3
  • Erika Maul
    • 1
  • Rudolf Eibach
    • 1
  • Reinhard Töpfer
    • 1
  • Eva M. Zyprian
    • 1
  1. 1.Federal Centre for Breeding Research on Cultivated PlantsInstitute for Grapevine Breeding GeilweilerhofSiebeldingenGermany
  2. 2.Faculty of Agriculture, Department of HorticultureSüleyman Demirel UniversityIspartaTurkey
  3. 3.Faculty of Agriculture, Department of Horticulture Ankara UniversityDiskapi, AnkaraTurkey

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