Molecular Breeding

, Volume 20, Issue 3, pp 261–270 | Cite as

Microsatellite markers flanking a stem solidness gene on chromosome 3BL in durum wheat

  • Saadollah Houshmand
  • Ronald E. Knox
  • Fran  R. Clarke
  • John  M. Clarke


Sawfly (Cephus cinctus Norton) is a major insect pest of wheat (Triticum spp.). The development of durum wheat (Triticum turgidum L. var durum) with stem solidness for resistance to sawfly is a strategy to minimize loss from this insect. This study was undertaken to identify a DNA marker linked to stem solidness and sawfly cutting in durum wheat for use in marker-assisted selection. A set of 151 doubled haploid lines developed from the cross of Kyle*2/Biodur sel. (solid stemmed) and Kofa (hollow stemmed) were evaluated for stem solidness and sawfly cutting. Microsatelite primers that generated polymorphisms between the parental genotypes were tested on the whole population, and primers that followed a 1:1 ratio of parental bands were used in linkage analysis with least squares mean stem solidness scores. Three microsatellite markers, Xgwm247, Xgwm181 and Xgwm114 located on chromosome 3BL, were shown to be associated with the stem solidness locus and with sawfly cutting. The Xgwm114 marker was located on one side of the stem solidness locus with Xgwm247 and Xgwm181 on the opposing side. Recombinant inbred line populations G9580B-FE1C/AC Navigator and Golden Ball/DT379//STD65 segregating for the stem solidness trait confirmed the association between the markers and the stem solidness gene. The Golden Ball/DT379//STD65 population was also tested with the Xwmc632 microsatellite marker, which showed a polymorphism associated with stem solidness. The results also indicated the stem solidness trait was controlled by a single locus in both doubled haploid and recombinant inbred line populations. The markers should be useful in breeding programs for the identification and selection of stem solidness.


Cephuscinctus Durum Microsatellite marker Sawfly Stem solidness Triticum 



We are grateful for the financial support of the Matching Investment Initiative of Agriculture and Agri-Food Canada and the Wheat Producer Check-Off administered by the Western Grains Research Foundation (WGRF) as well as an endowment grant provided by the WGRF. The technical assistance of Jay Ross, Shawn Yates, Brad Meyer, Isabelle Piché and Marlin Olfert, and marker and map information provided by Dr. Daryl Somers is appreciated.


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Saadollah Houshmand
    • 1
  • Ronald E. Knox
    • 2
  • Fran  R. Clarke
    • 2
  • John  M. Clarke
    • 2
  1. 1.Faculty of AgricultureUniversity of ShahrekordShahrekordIran
  2. 2.Agriculture and Agri-Food Canada, Semiarid Prairie Agricultural Research CentreSwift CurrentCanada

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