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Molecular Breeding

, Volume 17, Issue 1, pp 1–6 | Cite as

Fine Mapping of Xa2, a Bacterial Blight Resistance Gene in Rice

  • Qi He
  • Dongbo Li
  • Yongsheng Zhu
  • Mingpu Tan
  • Duanpin Zhang
  • Xinghua Lin
Article

Abstract

The rice bacterial blight resistance gene, Xa2, confers resistance to T7147 of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. It is located on the long arm of chromosome 4. Here, we report the fine mapping of Xa2 by genetic recombination analysis with simple sequence repeat (SSR) markers according to the genome sequence. Two F2 populations are constructed to localize Xa2. In a primary analysis with 136 random F2 plants of Zhenzhuai/IRBB2, it was found that Xa2 was located in approximately 20 cM region. To accurately determine the locus of Xa2, 120 new SSR markers were developed in this region by screening the sequence. Twelve new SSR markers were successfully used in genetic recombination analysis in IR24/IRBB2 population, while 20 in ZZA/IRBB2 population. We found that the nearest SSR markers to Xa2 are HZR950-5 and HZR970-4, which cover approximately 190-kb region. The sequence analysis of this 190-kb region revealed the presence of a homologous sequence of leucine rich repeat (LRR)-kinase. These results are very useful for transferring or pyramiding Xa2 by molecular marker-assistant selection in rice breeding programs and for cloning Xa2 by map-based cloning in combination with a long-range PCR strategy.

Keywords

Genetic map Oryza sativa L. Resistance gene Simple sequence repeat (SSR) Xanthomonas oryzae pv. oryzae 

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References

  1. Causse, M.A., Fulton, T.M., Cho, Y.G., Ahn, S.N., Chunwongse, J., Wu, K.S., Xiao, J.H., Yu, Z.H., Ronald, P.C., Harrington, S.E., Second, G., McCouch, S.R., Tanksley, S.D. 1994Saturated molecular map of the rice genome based on an interspecific backcross populationGenetics13812511274PubMedGoogle Scholar
  2. Chen, S., Lin, X.H., Xu, C.G., Zhang, Q.F. 2000Improvement of bacterial blight resistance of ‘Minghui 63’, an elite restorer line of hybrid riceby molecular marker-assisted selectionCrop Sci.40239244Google Scholar
  3. Chen, H., Wang, S., Zhang, Q. 2002New gene for bacterial blight resistance in rice located on chromosome 12 identified from Minghui 63, an elite restore linePhytopathology92750754Google Scholar
  4. Gnanamanickam, S.S., Priyadarisini, V.B., Narayanan, N.N., Vasudevan, P., Kavitha, S. 1999An overview of bacterial blight disease of rice and strategies for its managementCurrent Sci.7714351443Google Scholar
  5. Gu, K., Tian, D., Yang, F., Wu, L., Sreekala, C., Wang, D., Wang, G.L., Yin, Z. 2004High-resolution genetic mapping of Xa27(t), a new bacterial blight resistance gene in riceOryza sativa LTheor. Appl. Genet.108800807PubMedGoogle Scholar
  6. Harushima, Y., Yano, M., Shomura, A., Sato, M., Shimano, T., Kuboki, Y., Yamamoto, T., Lin, S.Y., Antonio, B.A., Parco, A., Kajiya, H., Huang, N., Yamamoto, K., Nagamura, Y., Kurata, N., Khush, G.S., Sasaki, T. 1998A high-density rice genetic linkage map with 2275 markers using a single F2 populationGenetics148479494PubMedGoogle Scholar
  7. Huang, N., Angeles, E.R., Domingo, J., Magpantay, G., Singh, S., Zhang, G., Kumaravadivel, N., Bennett, J., Khush, G.S. 1997Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCRTheor. Appl. Genet.95313320Google Scholar
  8. Kinoshita, T. 1995Report of committee on gene symbolization, nomenclature and linkage groupsRice Genet. Newslett.129153Google Scholar
  9. Kauffman, H.E., Reddy, A.P.K., Hsieh, S.P.Y., Merca, S.D. 1973An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzaePlant Disease Rep.57537541Google Scholar
  10. Lee, K.S., Rasabandith, S., Angeles, E.R., Khush, G.S. 2003Inheritance of resistance to bacterial blight in 21 cultivars of ricePhytopathology93147152Google Scholar
  11. Lin, X.H., Zhang, D.P., Xie, Y.F., Gao, H.P., Zhang, Q. 1996Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markersPhytopathology8611561159Google Scholar
  12. Lincoln S., Daly M. and Lander E. 1992. Constructing genetic maps with Mapmaker/Exp 3.0. 3rd ed. Whitehead Institute Technical Report, Cambridge, Massachusetts.Google Scholar
  13. McCouch, S.R., Teytelman, L., Xu, Y., Lobos, K.B., Clare, K., Walton, M., Fu, B., Maghirang, R., Li, Z., Xing, Y., Zhang, Q., Kono, I., Yano, M., Fjellstrom, R., Declerck, G., Schneider, D., Cartinhour, S., Ware, D., Stein, L. 2002Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.)DNA Res.9199207PubMedGoogle Scholar
  14. Ogawa, T., Morinaka, T., Fujii, K., Kimura, T. 1978Inheritance of resistance of rice varieties Kogyoku and Java 14 to bacterial group V of Xanthomonas oryzaeAnn. Phytopathol. Soc. Japan44137141Google Scholar
  15. Ogawa, T., Yamamoto, T., Khush, G.S., Mew, T.W., Kaku, H. 1988Near-isogenic lines as international differentials for resistance to bacterial blight of riceRice Genet. Newslett.5106107Google Scholar
  16. Ogawa, T., Yamamoto, T., Khush, G.S., Mew, T.W. 1991Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas campestris pv. oryzae)Japan J. Breed.41523529Google Scholar
  17. Panaud, O., Chen, X., McCouch, S.R. 1996Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)Mol. Gen. Genet.252597607PubMedGoogle Scholar
  18. Pryor, T. 1987The origin and structure of fungal disease resistance genes in plantsTrends Genet.3157161CrossRefGoogle Scholar
  19. Ronald, P.C., Albano, B., Tabien, R., Abenes, L., Wu, K., McCouch, S., Tanksley, S.D. 1992Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21Mol. Gen. Genet.236113120PubMedGoogle Scholar
  20. Rozen, S., Skaletsky, H. 2000Primer3 on the WWW for general users and for biologist programmersMethods Mol. Biol.132365386PubMedGoogle Scholar
  21. Sakaguchi, S. 1967Linkage studies on the resistance to bacterial leaf blightXanthomonas oryzae (Uyeda et Ishiyama) DOWSONin riceBull. Natl. Inst. Agr. Sci. Ser. D16118Google Scholar
  22. Song, J., Bradeen, J.M., Naess, S.K., Raasch, J.A., Weilgus, S.M., Haberlach, G.T., Liu, J., Kuang, H., Austin-Phillips, S., Buell, C.R., Helgeson, J.P., Jiang, J. 2003Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blightProc. Natl. Acad. Sci. USA10091289133PubMedGoogle Scholar
  23. Song, W.Y., Wang, G.L., Chen, L.L., Kim, H.S., Pi, L.Y., Holsten, T., Gardner, J., Wang, B., Zhai, W.X., Zhu, L.H., Fauquet, C., Ronald, P. 1995A receptor kinase-like protein encoded by the rice disease resistance gene Xa21Science27018041806PubMedGoogle Scholar
  24. Sun, X.L., Cao, Y.L., Yang, Z.F., Xu, C.G., Li, X.H., Wang, S.P., Zhang, Q.F. 2004Xa26a gene conferring resistance to Xanthomonas oryzae pv. oryzae in riceencodes an LRR receptor kinase-like proteinPlant J.37517527CrossRefPubMedGoogle Scholar
  25. Taura, S., Ogawa, T., Tabien, R.E., Khush, G.S., Yoshimura, A., Omura, T. 1987The specific reaction of Taichung Native 1 to Philippine race of bacterial blight and inheritance of resistance to race 5 (PX0112)Rice Genet. Newslett.4101102Google Scholar
  26. Temnykh, S., Park, W.D., Ayres, N., Cartinhour, S., Hauck, N., Lipovich, L., Cho, Y.G., Ishii, T., McCouch, S.R. 2000Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.)Theor. Appl. Genet.100697712CrossRefGoogle Scholar
  27. Wakimoto, S. 1954The determination of the presence of Xanthomonas oryzae by the phage techniqueSci. Bull. Fac. Agric. Kyushu Univ.14485493Google Scholar
  28. Yang, Z., Sun, X., Wang, S., Zhang, Q. 2003Genetic and physical mapping of a new gene for bacterial blight resistance in riceTheor. Appl. Genet.10614671472PubMedGoogle Scholar
  29. Yoshimura, S., Kurata, N., Sasaki, T., Yoshimura, A. 1994Genetic analysis of bacterial blight resistance genes in rice by using molecular markersRecent Adv. Breed.363336Google Scholar
  30. Yoshimura, S., Yoshimura, A., Iwata, N., McCouch, S.R., Abenes, M.L., Baraoidan, M.R., Mew, T.W., Nelson, R. 1995Tagging and combing bacterial blight resistance genes in rice using RAPD and RFLP markersMol. Breed.1375387CrossRefGoogle Scholar
  31. Yoshimura, S., Yamanouchi, U., Katayose, Y., Toki, S., Wang, Z.X., Kono, I., Kurata, N., Yano, M., Iwata, N., Sakaki, T. 1998Expression of Xa1a bacterial blight-resistance gene in riceis induced by bacterial inoculationProc. Natl. Acad. Sci. USA9516631668CrossRefPubMedGoogle Scholar
  32. Zhang, Q., Lin, S.C., Zhao, B.Y., Wang, C.L., Yang, W.C., Zhou, Y.L., Li, D.Y., Chen, C.B., Zhu, L.H. 1998Identification and tagging a new gene for resistance to bacterial blight (Xanthomonas oryzae pv. oryzae) from O. rufipogonRice Genet. Newslett.15138142Google Scholar
  33. Zhang, Q., Shen, B.Z., Dai, X.K., Mei, M.H., Saghai Maroof, M.A., Li, Z.B. 1994Using bulked extremes and recessive classes to map genes for photoperiod-sensitive genic male sterility in riceProc. Natl. Acad. Sci. USA9186758679PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Qi He
    • 1
  • Dongbo Li
    • 1
  • Yongsheng Zhu
    • 1
  • Mingpu Tan
    • 1
  • Duanpin Zhang
    • 1
  • Xinghua Lin
    • 1
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular BreedingHuazhong Agricultural UniversityWuhanChina

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