Molecular Breeding

, Volume 16, Issue 4, pp 271–278 | Cite as

Maternal Inheritance of Chloroplasts between Brassica rapa and F1-hybrids Demonstrated by cpDNA Markers Specific to Oilseed Rape and B. rapa

  • Marina M. Johannessen
  • Bente A. Andersen
  • Christian Damgaard
  • Rikke B. Jørgensen


Controlled reciprocal crosses between B. rapa and F1-hybrids (B. napus (♀) ×  B. rapa), giving 20 pair-crossings, were made to reveal possible irregularities in chloroplast inheritance during production of BC1s. Despite the close relationship of chloroplasts in B. rapa and B. napus, development of PCR-based molecular markers specific to B. rapa chloroplasts and B. napus chloroplasts was successful. Offspring from each cross were investigated and among these, we found no irregular chloroplast inheritance, since their plastid genotypes in all cases were identical to that of their mother. With a certainty of 95% our data indicate that the probability that chloroplasts are being inherited paternally is less than 0.015. In oilseed rape, pollen-mediated transgene-dispersal poses a well-known risk. Our results support development of transplastomic oilseed rape as an approach to reduce transgene dispersal.


Brassica napus PCR-RFLP (restriction fragment length polymorphism) Plastids Transplastomic 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Marina M. Johannessen
    • 1
  • Bente A. Andersen
    • 1
  • Christian Damgaard
    • 2
  • Rikke B. Jørgensen
    • 1
  1. 1.Plant Research DepartmentRisø National LaboratoryRoskildeDenmark
  2. 2.Department of Terrestrial EcologyNational Environmental Research InstituteSilkeborgDenmark

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