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Molecular Breeding

, Volume 16, Issue 4, pp 271–278 | Cite as

Maternal Inheritance of Chloroplasts between Brassica rapa and F1-hybrids Demonstrated by cpDNA Markers Specific to Oilseed Rape and B. rapa

  • Marina M. Johannessen
  • Bente A. Andersen
  • Christian Damgaard
  • Rikke B. Jørgensen
Article

Abstract

Controlled reciprocal crosses between B. rapa and F1-hybrids (B. napus (♀) ×  B. rapa), giving 20 pair-crossings, were made to reveal possible irregularities in chloroplast inheritance during production of BC1s. Despite the close relationship of chloroplasts in B. rapa and B. napus, development of PCR-based molecular markers specific to B. rapa chloroplasts and B. napus chloroplasts was successful. Offspring from each cross were investigated and among these, we found no irregular chloroplast inheritance, since their plastid genotypes in all cases were identical to that of their mother. With a certainty of 95% our data indicate that the probability that chloroplasts are being inherited paternally is less than 0.015. In oilseed rape, pollen-mediated transgene-dispersal poses a well-known risk. Our results support development of transplastomic oilseed rape as an approach to reduce transgene dispersal.

Keywords

Brassica napus PCR-RFLP (restriction fragment length polymorphism) Plastids Transplastomic 

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References

  1. Belzile, F.J. 2002Transgenic, transplastomic and othergenetically modified plants: a Canadian perspectiveBiochimie8411111118CrossRefPubMedGoogle Scholar
  2. Bewley, J.D., Hempel, F.D., McCormick, S., Zambryski, P. 2000Reproductive developmentBuchanan, B.B.Gruissem, W.Jones, R.L. eds. Biochemistry & Molecular Biology of PlantsThe American Society of Plant PhysiologistsUSA9881043Google Scholar
  3. Bock, R. 2001Transgenic plastids in basic research and plant biotechnologyJ. Mol. Biol.312425438CrossRefPubMedGoogle Scholar
  4. Carlin, B.P., Louis, T.A. 1996Bayes and Empirical Bayes Methods for Data AnalysisChapman & HallLondonGoogle Scholar
  5. Chamberlain, D., Stewart, C.N. 1999Transgene escape and transplastomicsNat. Biotechnol.17330331CrossRefPubMedGoogle Scholar
  6. Charters, Y.M., Robertson, A., Wilkinson, M.J., Ramsay, G. 1996PCR analysis of oilseed rape cultivars (Brassica napus L. ssp oleifera) using 5′-anchored simple sequence repeat(SSR) primersTheor. Appl. Genet.92442447Google Scholar
  7. Corriveau, J.L., Coleman, A.W. 1988Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm speciesAm. J. Bot.7514431458Google Scholar
  8. Daniell, H., Khan, M.S., Allison, L. 2002Milestones inchloroplast genetic engineering: an environmentally friendly era in biotechnologyTrends Plant Sci.78491CrossRefPubMedGoogle Scholar
  9. Daniell, H., Muthukumar, B., Lee, S.B. 2001Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selectionCurr. Genet.39109116CrossRefPubMedGoogle Scholar
  10. Cosa, B., Moar, W., Lee, S.B., Miller, M., Daniell, H. 2001Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystalsNat. Biotechnol.197174PubMedGoogle Scholar
  11. Hansen, L.B., Siegismund, H.R., Jørgensen, R.B. 2001Introgression between oilseed rape (Brassica napus L.) and its weedy relative B. rapa L in a natural populationGenet. Resour. Crop Evol.48621627CrossRefGoogle Scholar
  12. Hansen, L.B., Siegismund, H.R., Jørgensen, R.B. 2003Progressive introgression between Brassica napus (oilseed rape) and B. rapaHeredity91276283CrossRefPubMedGoogle Scholar
  13. Haygood, R., Ives, A.R., Andow, D.A. 2004Population genetics of transgene containmentEcol. Lett.7213220CrossRefGoogle Scholar
  14. Hou, B.K., Zhou, Y.H., Wan, L.H., Zhang, Z.L., Shen, G.F., Chen, Z.H., Hu, Z.M. 2003Chloroplast transformation in oilseed rapeTransgenic Res.12111114CrossRefPubMedGoogle Scholar
  15. Johannessen, M.M., Mikkelsen, T.N., Jørgensen, R.B. 2002CO2exploitation and genetic diversity in winter varieties of oilssed rape (Brassica napus); varieties of tomorrowEuphytica1287586Google Scholar
  16. Jørgensen, R.B., Andersen, B. 1994Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B.campestris (Brassicaceae): a risk of growing genetically modified oilseed rapeAm. J. Bot.8116201626Google Scholar
  17. Khan, M.S., Maliga, P. 1999Flourescent antibiotic resistance marker for tracking plastid transformation in higher plantsNat. Biotechnol.17910914PubMedGoogle Scholar
  18. Kiang, A.-S., Connolly, V., McConnell, D., Kavanagh, T. 1994Paternal inheritance of mitochondria and chloroplasts in Festucapratensis-Loliumperenne intergeneric hybridsTheor. Appl. Genet.87681688CrossRefGoogle Scholar
  19. Landbo, L., Jørgensen, R.B. 1997Seed germination in weedy Brassica campestrisits hybrids with B. napus; Implications for risk assesment of oilseed rapeEuphytica.97209216CrossRefGoogle Scholar
  20. Lu, G.Y., Yang, G.S., Fu, T.D. 2004Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassicanapus)Plant Breed.123262265Google Scholar
  21. Mikkelsen, T.R., Andersen, B., Jørgensen, R.B. 1996The risk of crop transgene spreadNature38031CrossRefGoogle Scholar
  22. Milligan, B.G. 1998Total DNA isolationHoelzel, A.R. eds. Molecular Genetic Analysis of Populations: A Practical ApproachIRL PressOxford2964Google Scholar
  23. Mogensen, H.L. 1996The hows and whys of cytoplasmic inheritance in seed plantsAm. J. Bot.83383404Google Scholar
  24. Murgia, M., Detchepare, S., Vanwent, J.L., Cresti, M. 1991Brassica napus pollen development during generative cell and sperm cell-formationSex. Plant Reprod.4176181Google Scholar
  25. Pertl, M., Hauser, T.P., Damgaard, C., Jørgensen, R.B. 2002Male fitness of oilseed rape (Brassica napus), weedy B.rapatheir F1 hybrids when pollinating B. rapa seedsHeredity89212218CrossRefPubMedGoogle Scholar
  26. Pradhan, A.K., Prakash, S., Mukhopadhyay, A., Pental, D. 1992Phylogeny of Brassicaallied genera based on variation in chloroplast and mitochondrial DNA patterns – Molecular and taxonomic classifications are incongruousTheor. Appl. Genet.85331340CrossRefGoogle Scholar
  27. Reboud, X., Zeyl, C. 1994Organelle inheritance in plantsHeredity72132140Google Scholar
  28. Ruf, S., Hermann, M., Berger, I.J., Carrer, H., Bock, R. 2001Stable genetic transformation of tomato plastids and expression of a foreign protein in fruitNat. Biotechnol.19870875CrossRefPubMedGoogle Scholar
  29. Scott, S., Wilkinson, M. 1999Low probability of chloroplast movement from oilseed rape (Brassica napus) into wild Brassica rapaNat. Biotechnol.17390393CrossRefPubMedGoogle Scholar
  30. Sidorov, V.A., Kasten, D., Pang, S.Z., Hajdukiewicz, P.T.J., Staub, J.M., Nehra, N.S. 1999Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid markerPlant J.19209216CrossRefPubMedGoogle Scholar
  31. Sikdar, S.R., Serino, G., Chaudhuri, S., Maliga, P. 1998Plastid transformation in Arabidopsis thalianaPlant Cell Rep.182024CrossRefGoogle Scholar
  32. Skarjinskaia, M., Svab, Z., Maliga, P. 2003Plastid transformation in Lesquerella fendlerian oilseed BrassicaceaTransgenic Res.12115122CrossRefPubMedGoogle Scholar
  33. Somanchi, A., Mayfield, S.P. 1999Nuclear-chloroplast signallingCurr. Opin. Plant Biol.2404409CrossRefPubMedGoogle Scholar
  34. Spillane, C., Curtis, M.D., Grossniklaus, U. 2004Apomixis technology development – virgin births in farmers′ fields?Nat. Biotechnol.22687691CrossRefPubMedGoogle Scholar
  35. Staniland, B.K., Mcvetty, P.B.E., Friesen, L.F., Yarrow, S., Freyssinet, G., Freyssinet, M. 2000Effectiveness of border areas in confining the spread of transgenic Brassica napus pollenCan. J. Plant Sci.80521526Google Scholar
  36. Svab, Z., Hajdukiewicz, P.T.J., Maliga, P. 1990Stable transformation of plastids in higher-plantsProc. Natl. Acad. Sci. USA8785268530PubMedGoogle Scholar
  37. Taberlet, P., Gielly, L., Pautou, G., Bouvet, J. 1991Universal primers for amplification of three non-coding regions of chloroplast DNAPlant Mol. Biol.1711051109CrossRefPubMedGoogle Scholar
  38. Tolstrup K., Andersen S.B., Boelt B., Buus M., Gylling M. Holm P.B., Kjellson G., Pedersen S., Østergard H., Mikkel- sen S.A.Report from the Danish working group on the co- existence of genetically modified crops with conventional organic crops. 2003. Frederiksberg Bogtryk. DIAS report, Plant Production Ref Type: Report.Google Scholar
  39. U.N.1935Genome-analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilizationJpn. J. Bot.VII389453Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Marina M. Johannessen
    • 1
  • Bente A. Andersen
    • 1
  • Christian Damgaard
    • 2
  • Rikke B. Jørgensen
    • 1
  1. 1.Plant Research DepartmentRisø National LaboratoryRoskildeDenmark
  2. 2.Department of Terrestrial EcologyNational Environmental Research InstituteSilkeborgDenmark

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