The selection and properties of Penicillium verruculosum mutants with enhanced production of cellulases and xylanases
The paper describes three Penicillium verruculosum 28K mutants with about threefold enhanced production of five industrially important carbohydrases. The two-stage fermentation process that we developed provided a further two- to threefold increase in the production of carbohydrases. Physiological and biochemical studies showed that the synthesis of all five carbohydrases is inducible. Carboxymethylcellulase, xylanase, and β-glucanase are synthesized under a common regulatory control, as is evident from the concurrent increase in the synthesis of these enzymes in the presence of microcrystalline cellulose. The synthesis of avicelase and β-glucosidase is evidently induced by other cellulose- and hemicellulose-containing compounds present in the fermentation medium and, hence, is regulated independently of the three aforementioned enzymes.
Key wordscellulase xylanase mutants fed-batch cultivation
Unable to display preview. Download preview PDF.
- 6.Gomarteli, M., Kvesitadze, E., Bezborodov, A.M., Shkol’nyi, A.T., Dzhobava, M., and Adeishvili, E., Mutants of Penicillium canescens with Enhanced Xylanase Activity, Prikl. Biokhim. Mikrobiol., 1998, vol. 34, pp. 650–654.Google Scholar
- 7.Kastel’yanos, O.F., Ermolova, O.V., Sinitsyn, A.P., Popova, N.N., Okunev, O.N., Kerns, G., and Kude, E., Purification of Enzymes of the Cellulase Complex of Penicillium verruculosum and the Study of Their Biochemical Properties and Specificity, Biokhimiya, 1995, vol. 60, pp. 925–943.Google Scholar
- 10.Sinitsyn, A.P., Chernoglazov, V.M., and Gusakov, A.V., Metody opredeleniya aktivnosti tsellyulaz, Itogi Nauki Tekh., Ser. Biotekhnol., 1993, vol. 25, pp. 38–64.Google Scholar
- 11.Nelson, A., A Photometric Adaptation of the Determination of Reducing Sugars, J. Biol. Chem., 1944, vol. 153, pp. 375–380.Google Scholar
- 12.Somogy, M., A New Reagent for the Determination of Sugars, J. Biol. Chem., 1945, vol. 160, pp. 61–68.Google Scholar
- 17.Nevalainen, H. and Pentilla, M., Molecular Biology of Cellulolytic Fungi, The Mycota: II. Genetic and Biotechnology, Esser, K. and Lemke, P.A., Eds., Berlin: Springer, 1995.Google Scholar
- 18.Kurasawa, T., Yachi, M., Suto, M., Kamagata, Y., Takao, S., and Tomita, F., Induction of Cellulase by Gentiobiose and Its Sulfur-Containing Analog in Penicillium purpurogenum, Appl. Environ. Microbiol., 1992, vol. 58, pp. 106–110.Google Scholar