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Therapeutic effects of probiotics on neurotoxicity induced by clindamycin and propionic acid in juvenile hamsters

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A Correction to this article was published on 31 August 2018

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Abstract

The present study investigated the therapeutic effects of probiotics on brain intoxication induced by clindamycin and propionic acid (PPA) in hamsters. Fifty golden Syrian hamsters were randomly divided into five experimental groups of ten animals each: (A) control group receiving phosphate buffered saline; (B) oral buffered PPA-treated group being administered with a neurotoxic dose of 250 mg/kg PPA during three days; (C) oral clindamycin-treated group receiving a single dose of 30 mg clindamycin/kg; and (D, E) the two therapeutic groups being administered the same doses of clindamycin and PPA followed by probiotics for three weeks at a daily dose of 0.2 g/kg. Biochemical parameters of energy metabolism and oxidative stress were examined in brain homogenates from all hamsters. The development of pathogenic bacteria was monitored on stool samples from all hamsters. Descriptive changes in fecal microbiota and overgrowth of Clostridium species in clindamycin and PPA treated hamsters were recorded. Interestingly, probiotics were shown effective to restore normal gut microbiota. Clindamycin and PPA treatments caused an elevation in lipid peroxidation and catalase activity, as oxidative stress markers, together with a reduction in GST activity and GSH level. Energy metabolism impairment was ascertained via the activation of creatine kinase and a decrease of lactate dehydrogenase. These findings suggest that bacteria overgrowth caused by PPA and clindamycin was efficient to illustrate signs of neuronal toxicity. The present study indicates that probiotic treatment can improve poor detoxification, oxidative stress, and altered gut microbiota as mechanisms implicated in the etiology of many neurological disorders.

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Change history

  • 31 August 2018

    The original version of this article unfortunately contained a mistake. The family name of the fourth author listed in the title was incorrect, and the correct name is Nadine Moubayed, as noted in the addresses. Her name is now corrected in the author group of this article.

Abbreviations

AUC :

area under the curve

BAP :

Sheep Blood Agar plate

BBB :

blood-brain barrier

CDNB :

1-chloro-2,4-dinitrobenzene

CFU :

Colony-forming unit

CK :

creatine kinase

CNS :

center nerves system

ENS :

enteric nervous system

ETC :

electron transport chain

GI :

gastrointestinal tract

GSH :

glutathione

GST :

glutathione-s-transferase

H 2 O 2 :

hydrogen peroxide

HK :

hexokinase

LDH :

lactate dehydrogenase

MCA :

MacConkey Agar

MD :

malondialdehyde

PPA :

propionic acid

ROC :

Receiver Operating Characteristics

ROS :

reactive oxygen species

SCFA :

Short-Chain Fatty Acids

SDA :

Sabouroud Dextrose agar

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Acknowledgments

This research project was supported by a grant from the Research Center of the Female Scientific and Medical Colleges, Deanship of Scientific Research, King Saud University.

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Authors and Affiliations

  1. Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

    Nora Al-Orf, Ramesa Shafi Bhat & Abir Ben Bacha

  2. The Materials Science Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia

    Nora Al-Orf

  3. Central Laboratory, King Saud University, Riyadh, Saudi Arabia

    Afaf El-Ansary

  4. Council for Nutritional and Environmental Medicine, Toften 24, 8610, Mo i Rana, Norway

    Geir Bjørklund

  5. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Nadine Moubayed

  6. Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Science of Sfax, University of Sfax, Sfax, Tunisia

    Abir Ben Bacha

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  1. Nora Al-Orf
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Correspondence to Geir Bjørklund.

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The authors declare no potential conflicts of interest with respect to the authorship, and/or publication of this article.

Ethical approval

This study was ethically approved by the Ethical Committee, College of Science, King Saud University.

Additional information

The original version of this article was revised: The family name of the fourth author listed in the title was incorrect, and the correct name is Nadine Moubayed, as noted in the addresses. Her name is now corrected in the author group of this article.

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Al-Orf, N., El-Ansary, A., Bjørklund, G. et al. Therapeutic effects of probiotics on neurotoxicity induced by clindamycin and propionic acid in juvenile hamsters. Metab Brain Dis 33, 1811–1820 (2018). https://doi.org/10.1007/s11011-018-0284-5

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  • DOI: https://doi.org/10.1007/s11011-018-0284-5

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