Abstract
Inclusion of vegetables in the diet not only provides dietary fiber, vitamins, minerals, trace elements but also significantly reduces the risk of several diseases. Raphanus sativus L. Var. caudatus belongs to the family Brassicaceae are pods of Radish, and are commonly known as Mungra or Sungra in Pakistan and India. The English name for this species is Rat-tailed radish. This variety of radish is unique, less familiar to the population, and not commonly used as a food source. Furthermore there have been very few studies that report on the potential antioxidant and anti-cancer capabilities of this radish. The present study was designed to evaluate anxiolytic potential of Raphanus caudatus in mice using different behavioral paradigms. The ethanol extract of the plant was evaluated at three different doses i.e. 250, 500 and 1000 mg/kg. The extract at doses of 250 and 500 mg/kg produced a significant (p < 0.05) reduction of anxiety-like behavior in mice and results are comparable to standard anxiolytic drug diazepam.
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References
Aguirre-Hernández E, González-Trujano ME, Terrazas T, Santoyo JH, Guevara-Fefer P (2016) Anxiolytic and sedative-like effects of flavonoids from Tilia americana var. mexicana: GABAergic and serotonergic participation. Salud Mental 39(1):37–46
Bigliani MC, Rosso MC, Zunino PM, Baiardi G, Ponce AA (2013) Anxiogenic-like effects of Uncaria tomentosa (Willd.) DC. aqueous extract in an elevated plus maze test in mice: a preliminary study. Nat Prod Res 27(18):1682–1685
Charoonratana T, Settharaksa S, Madaka F, Songsak T (2014) Screening of antioxidant activity and total phenolic content in Raphanus sativus pod. Int J Pharm Pharm Sci 6(8):224–226
Crawley J, Goodwin FK (1980) Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepines. Pharmacol Biochem Behav 13(2):167–170
Doukkali Z, Taghzouti K, Bouidida EH, Nadjmouddine M, Cherrah Y, Alaoui K (2015) Evaluation of anxiolytic activity of methanolic extract of Urtica urens in a mice model. Behav Brain Funct 11(1):19
Farrukh A, Iqbal A, Zafar M (2006) Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 30:177–183
Guideline O (2001) 423: Acute oral toxicity-acute toxic class method. OECD Guidelines for the Testing of Chemicals. OECD. OECD Publishing, Paris, France, p 1–14
Han JM, Lee YJ, Lee SY, Kim EM, Moon Y, Kim HW, Hwang O (2007) Protective effect of sulforaphane against dopaminergic cell death. J Pharmacol Exp Ther 321(1):249–256
Hosein Farzaei M, Bahramsoltani R, Rahimi R, Abbasabadi F, Abdollahi M (2016) A systematic review of plant-derived natural compounds for anxiety disorders. Curr Top Med Chem 16(17):1924–1942
Khattak KF (2011) Nutrient composition, phenolic content and free radical scavenging activity of some uncommon vegetables of Pakistan. Pak J Pharm Sci 24(3):277–283
Kim KH, Moon E, Kim SY, Choi SU, Lee JH, Lee KR (2014) 4-Methylthio-butanyl derivatives from the seeds of Raphanus sativus and their biological evaluation on anti-inflammatory and antitumor activities. J Ethnopharmacol 151(1):503–508
Kulkarni S (1999) Handbook of Experimental Pharmacology. 3rd rev. Ed. Vallabh Prakashan, New Delhi, pp 123–125
Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, Paladini AC (1997) Overview—flavonoids: a new family of benzodiazepine receptor ligands. Neurochem Res 22(4):419–425
Melo CTV d, Monteiro AP, Leite CP, Araújo FLO d, Lima VTM, Barbosa-Filho JM et al (2006) Anxiolytic-like effects of (O-methyl)-N-2, 6-dihydroxybenzoyl-tyramine (riparin III) from Aniba riparia (Nees) Mez (Lauraceae) in mice. Biol Pharm Bull 29(3):451–454
Mesfin M, Asres K, Shibeshi W (2014) Evaluation of anxiolytic activity of the essential oil of the aerial part of Foeniculum vulgare Miller in mice. BMC Complement Altern Med 14(1):310
Okoduwa SIR, Umar IA, James DB, Inuwa HM, Habila JD (2016) Evaluation of extraction protocols for anti-diabetic phytochemical substances from medicinal plants. World J Diabetes 7(20):605
Reynolds E (2003) Brain and mind: a challenge for WHO. Lancet 361(9373):1924–1925
Sethi A, Das B, Bajaj B (2005) The anxiolytic activity of gabapentin in mice. J Appl Res Clin Exp Ther 5(3):415
Siddiqui NA, Ahmad N, Musthaq N, Chattopadhyaya I, Kumria R, Gupta S (2014) Neuropharmacological profile of extracts of aerial parts of Convolvulus pluricaulis Choisy in mice model. Open Neurol J 8:11
Sultana N, Najam R (2012) Anxiolytic activity of Aloe vera (L.) Burm. F tested in rodents. Pak J Pharmacol 29(1):7–15
Treit D, Fundytus M (1988) Thigmotaxis as a test for anxiolytic activity in rats. Pharmacol Biochem Behav 31(4):959–962
Wu S, Gao Q, Zhao P, Gao Y, Xi Y, Wang X et al (2016) Sulforaphane produces antidepressant-and anxiolytic-like effects in adult mice. Behav Brain Res 301:55–62
Zhang Z-J (2004) Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 75(14):1659–1699
Zhao J, Kobori N, Aronowski J, Dash PK (2006) Sulforaphane reduces infarct volume following focal cerebral ischemia in rodents. Neurosci Lett 393(2):108–112
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Authors are thankful to Department of Pharmacology, University of Karachi for providing facilities to conduct this research.
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All the research protocol, procedures, euthanasia as well as ethical clearance were approved by the BASR (Board of Advanced Studies & Research), University of Karachi (BASR number 02419/Pharm). All the selected animals were handled using ethical principles of research. Specifications described in Helsinki Resolution 1964 were adopted for handling of animals in the current research.
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Siddiq, A., Younus, I. The Radish, Raphanus sativus L. Var. caudatus reduces anxiety-like behavior in mice. Metab Brain Dis 33, 1255–1260 (2018). https://doi.org/10.1007/s11011-018-0240-4
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DOI: https://doi.org/10.1007/s11011-018-0240-4