Skip to main content

Advertisement

Log in

ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells

Metabolic Brain Disease Aims and scope Submit manuscript

Abstract

Ionizing radiation induces various pathophysiological conditions by altering central nervous system (CNS) homeostasis, leading to neurodegenerative diseases. However, the potential effect of ionizing radiation response on cellular physiology in glial cells is unclear. In the present study, micronucleus test, comet assay, and RT-PCR were performed to investigate the potential effect of gamma radiation in cultured oligodendrocytes and astrocytes with respect to genomic instability, Endoplasmic Reticulum (ER) stress, and inflammation. Further, we studied the effect of alteration in ER stress specific gene expression in cortex post whole body radiation in mice. Results showed that exposure of gamma radiation of 2Gy in-vitro cultured astrocytes and oligodendrocytes and 7Gy in-vivo induced ER stress and Inflammation along with profuse DNA damage and Chromosomal abnormality. Additionally, we observed downregulation of myelin basic protein levels in cultured oligodendrocytes exposed to radiation. The present data suggests that ER stress and pro inflammatory cytokines serve as the major players in inducing glial cell dysfunction post gamma irradiation along with induction of genomic instability. Taken together, these results indicate that ER stress, DNA damage, and inflammatory pathways may be critical events leading to glial cell dysfunction and subsequent cell death following exposure to ionizing radiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Abbreviations

Bcl 2:

B-cell lymphoma 2

BIP:

Binding immunoglobulin protein

CASP:

Comet assay software project

CCK:

Cell Counting Kit

DAPI:

4′,6-diamidino-2-phenylindole

DMEM:

Dulbecco′s modified eagle′s medium

DMSO:

Dimethyl sulfoxide

DNA:

Deoxyribonucleic acid

FBS:

Fetal bovine serum

FITC:

Fluorescein isothiocyanate

Gadd153:

DNA damage-inducible gene 153

GFAP:

Glial fibrillary acidic protein

Gy:

Gray

MMW:

Millimeter wave

PGE 2:

Prostaglandin E2

RT-PCR:

Reverse transcription polymerase chain reaction

TRITC:

Tetramethylrhodamine

References

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mohammed S. Mustak.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chatterjee, J., Nairy, R.K., Langhnoja, J. et al. ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells. Metab Brain Dis 33, 855–868 (2018). https://doi.org/10.1007/s11011-018-0183-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11011-018-0183-9

Keywords

Navigation