Metabolic Brain Disease

, Volume 33, Issue 3, pp 725–731 | Cite as

Effect of garlic powder on hippocampal long-term potentiation in rats fed high fat diet: an in vivo study

  • Iraj Salehi
  • Alireza Komaki
  • Seyed Asaad Karimi
  • Abdolrahman Sarihi
  • Mohammad Zarei
Original Article


The objective of this study was to determine the relation between the chronic consumption of garlic powder in combination with high-fat diet (HFD) on long term potentiation (LTP) in the dentate gyrus (DG) of rat hippocampus. Male rats were divided to 4 groups, control with the standard diet, control with a standard diet plus garlic, high-fat diet (HFD) group and high-fat diet with garlic. Following 6 months of controlled dietary in each experimental group, the rats were anesthetized with i.p. injection of ketamine and xylazin (100 and 2.5 mg/kg, respectively), and placed into a stereotaxic apparatus for surgery, electrode implantation and field potential recording. The population spike (PS) amplitude and slope of excitatory post synaptic potentials (EPSP) were measured in the DG area of adult rats in response to stimulation applied to the perforant path (PP) (by 400 Hz tetanization). The results showed that garlic increased EPSP slope and PS amplitude respect to HFD group. It was suggested that the garlic powder administration could attenuate the deteriorating effect of HFD on in vivo hippocampal LTP in the granular cells of the DG.


Hippocampus Dentate gyrus Long-term potentiation High fat diet Garlic Powder 



The authors would like to express their gratitude to the staff of the Neurophysiology Research Center for helping us to carry out this project. The research was supported in part by grants of Hamadan University of Medical Sciences and Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Compliance with ethical standards

Conflict of interest

We confirm that the authors do not have any conflict of interest with this publication.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Iraj Salehi
    • 1
  • Alireza Komaki
    • 1
  • Seyed Asaad Karimi
    • 1
    • 2
  • Abdolrahman Sarihi
    • 1
  • Mohammad Zarei
    • 1
  1. 1.Neurophysiology Research CenterHamadan University of Medical SciencesHamadanIran
  2. 2.Department of Physiology and Neurophysiology Research Center, School of MedicineShahid Beheshti University of Medical SciencesTehranIran

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