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Metabolic Brain Disease

, Volume 33, Issue 2, pp 601–613 | Cite as

Hypothalamic-pituitary-adrenal axis variants and childhood trauma influence anxiety sensitivity in South African adolescents

  • Jacqueline S. Womersley
  • Lindi I. Martin
  • Lize van der Merwe
  • Soraya Seedat
  • Sian M. J. Hemmings
Original Article

Abstract

Anxiety sensitivity (AS) is characterised by the fear of anxiety-related symptoms and is a risk factor for the development of anxiety-related disorders. We examined whether genetic variation in three stress response genes, CRHR1, NR3C1, and FKBP5, interact with childhood trauma (CT) to predict AS in South African adolescents. Xhosa (n = 634) and Coloured (n = 317) students completed self-report measures of AS and CT, and a total of eighteen polymorphisms within CRHR1, NR3C1, and FKBP5 were genotyped. Differences in AS based on genetic variation and CT were analysed within population and gender groups using multiple linear regression. Associations were found between AS and FKBP5 rs9296158 (p = 0.025) and rs737054 (p = 0.045) in Coloured males. Analysis of gene x CT interactions indicated that NR3C1 rs190488 CC-genotype, NR3C1 rs10482605 G-allele addition, and FKBP5 rs3800373 C-allele addition protect against AS with increasing CT in Xhosa females (p = 0.009), Xhosa males (p = 0.036) and Coloured males (p = 0.049), respectively. We identified two different protective single nucleotide polymorphism (SNP) combinations in a four-SNP CRHR1 haplotype in Coloured males. An analysis of the interaction between CT and a six-SNP FKBP5 haplotype in Coloured males revealed both protective and risk allelic combinations. Our results provide evidence for the influence of both genetic variation in CRHR1, NR3C1 and FKBP5, as well as CT x SNP interactions, on AS in South African adolescents. This study reinforces the importance of examining the influence of gene-environment (G X E) interactions within gender and population groups.

Keywords

Anxiety CRHR1 FKBP5 Genetics Hypothalamic-pituitary-adrenal axis NR3C1 

Notes

Acknowledgements

This study was supported by a South African Medical Research Council (MRC) Self-initiated Research grant (PI: S. Hemmings). This work is based upon research supported by the South African Research Chairs Initiative in PTSD of the Department of Science and Technology and the National Research Foundation.

Compliance with ethical standards

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendment or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

11011_2017_138_MOESM1_ESM.docx (24 kb)
Supplementary Table 1 (DOCX 24 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Jacqueline S. Womersley
    • 1
  • Lindi I. Martin
    • 1
  • Lize van der Merwe
    • 2
  • Soraya Seedat
    • 1
  • Sian M. J. Hemmings
    • 1
  1. 1.Department of Psychiatry, Faculty of Medicine and Health SciencesStellenbosch UniversityTygerbergSouth Africa
  2. 2.Department of Human Genetics, Faculty of AgricultureStellenbosch UniversityStellenboschSouth Africa

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