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Metabolic Brain Disease

, Volume 33, Issue 2, pp 369–375 | Cite as

Curcumin-supplemented diets improve antioxidant enzymes and alter acetylcholinesterase genes expression level in Drosophila melanogaster model

  • Ayodele Jacob Akinyemi
  • Ganiyu Oboh
  • Opeyemi Ogunsuyi
  • Amos Olalekan Abolaji
  • Adetutu Udofia
Original Article

Abstract

Curcumin, a bioactive polyphenolic compound in turmeric (Curcuma longa) rhizomes has been shown to exert anti-aging properties with limited scientific basis. Hence, this study sought to examine the antioxidant and anti-cholinesterase activities of curcumin-supplemented diets as well as their molecular effect on superoxide dismutase (SOD) and acetylcholinesterase (AChE) genes expression level associated with lifespan extension in Drosophila melanogaster model. In this experiment, D. melanogaster (both genders) of 1 to 3 days old were fed diets either containing no curcumin (control) or supplemented with curcumin at 0.2 and 1.0 mg/g of diet for 7 days. Subsequently, the survival and locomotor activities were determined. In addition, we evaluated RT-PCR expressions of SOD and AChE mRNA genes. Furthermore, catalase, SOD and AChE activities were determined. Curcumin-supplemented diet improves survival ability but did not affect locomotor activity when compared with the control. In addition, there was a significant increase in SOD and catalase with a concomitant decrease of AChE activities when compared with the control. Furthermore, curcumin-supplemented diets suppress AChE mRNA expression but no alteration on SOD gene expression level was observed when compared with control. In conclusion, our present results suggest that a down-regulation of AChE gene expression with a concomitant decrease of AChE activity as well as improving antioxidant status could be some possible mechanism in which curcumin exert anti-aging potential and increases lifespan of D. melanogaster.

Keywords

Curcumin D. melanogaster Acetylcholinesterase Superoxide dismutase Aging 

Notes

Acknowledgments

One of the authors (Ayodele Jacob Akinyemi) is a beneficiary of 2016 IBRO/ARC bursary award and wish to thank the organization for their support towards this study.

Compliance with ethical standards

Conflict of interest

No conflict of interest to be stated for any of the authors.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Ayodele Jacob Akinyemi
    • 1
    • 2
  • Ganiyu Oboh
    • 2
  • Opeyemi Ogunsuyi
    • 2
    • 3
  • Amos Olalekan Abolaji
    • 4
  • Adetutu Udofia
    • 5
  1. 1.Department of Biochemistry, College of SciencesAfe Babalola University Ado-EkitiAdo EkitiNigeria
  2. 2.Department of BiochemistryFederal University of TechnologyAkureNigeria
  3. 3.Department of Biomedical TechnologyFederal University of TechnologyAkureNigeria
  4. 4.Department of Biochemistry, Faculty of Basic Medical Sciences, College of MedicineUniversity of IbadanIbadanNigeria
  5. 5.Department of Bioscience CenterInternational Institute of Tropical AgricultureIbadanNigeria

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