Skip to main content
SpringerLink
Log in

Changes in the brain antioxidant profile after chronic vanadium administration in mice

  • Original Article
  • Published:
Metabolic Brain Disease Aims and scope Submit manuscript

Abstract

Vanadium is known to induce reactive oxygen species (ROS) in biological systems. Exposure to vanadium has been linked to neurological defects affecting the central nervous system (CNS) early in life and culminates later to neurodegeneration. This study was designed to evaluate the effects of chronic vanadium exposure on antioxidant profile in mice, and progressive changes after withdrawal from treatment. A total of 85 male BALB/c mice (4 weeks old) were used for the experiment and were divided into three groups of vanadium exposed (3 mg/kg i.p at 3–18 months treatment), matched controls, and animals exposed to vanadium for three months and thereafter vanadium was withdrawn. Vanadium exposure caused significant increases (p<0.05) in levels of malondialdehyde (MDA), hydrogen peroxide (H2O2) generation and nitric oxide with a concomitant decrease (p<0.05) in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-S-transferase and a decline in the level of reduced glutathione (GSH) after 6 months of vanadium exposure in the brain. This trend continued in all vanadium-exposed groups (9, 12, 15 and 18 months) relative to the matched controls. Withdrawal after 3 months of vanadium exposure significantly reversed oxidative stress in intoxicated mice from 9 to 15 months after vanadium withdrawal. We have shown that chronic administration of vanadium led to oxidative stress in the brain which is reversible only after a long period of vanadium withdrawal.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Adebiyi OE, Obisesan AD, Olayemi FO, Olopade JO (2015) Protective effect of ethanolic extract of Grewia Carpinifolia leaves on vanadium induced toxicity. Alex J Vet Sci 47:1–6

    Google Scholar 

  • Adejuwon SA, Imosemi IO, Ebokaiwe PA, Omirinde JO, Adenipekun AA (2014) Protective role of Telfairia Occidentalis in irradiation-induced oxidative stress in rat brain. Int J Biol Chem Sci 3(8):843–853

    Article  Google Scholar 

  • Amorim FAC, Welz B, Costa ACS, Lepri FG, Vale MGR, Ferreira SLC (2007) Determination of vanadium in petroleum and petroleum products using atomic spectrometric techniques. Talanta 72:349–359

    Article  CAS  PubMed  Google Scholar 

  • Avila-Costa MR, Zepeda-Rodríquez A, Antuna SB, Saldivar OL, Espejel-Maya G, Mussali-Galante P, Avila-Casado MC, Reyes-Olivera A, Anaya-Martinez V, Fortoul TI (2005) Ependymal epithelium disruption after vanadium pentoxide inhalation: a mice Experimental model. Neurosci Lett 381:21–25

  • Aydin A, Sayal A, Sayin S, Erdem O (2005) An investigation on the relationship between vanadium and antioxidative enzyme system in rats. Turk J Pharm Sci 2:17–24

    CAS  Google Scholar 

  • Azeez IA, Olopade F, Laperchia C, Andrioli A, Scambi I, Onwuka SK, Marina B, Olopade JO (2016) Regional myelin and axon damage and Neuroinflammation in the adult mouse brain after long-term postnatal vanadium exposure. J Neuropathol Exp Neurol 75:843–854

    Article  CAS  PubMed  Google Scholar 

  • Bast A, Barr PR (1997) The antioxidant/prooxidant balance in neurodegeneration. In: Bar PR, Beal MF (eds) Neuroprotection in CNS diseases. Marcel Dekker, New York, pp 147–159

    Google Scholar 

  • Brendt DS (1999) Endogenous nitric oxide synthesis: biological functions and pathophysiology. Free Radic Res J 31:577–596

    Article  Google Scholar 

  • Briner W (2014) The Alchemist’s approach to metal poisoning: transforming the metal burden. Toxics 2:364–376

    Article  Google Scholar 

  • Cairney S, Maruff P, Burns CB, Currie J, Currie BJ (2005) Neurological and cognitive recovery following abstinence from petrol sniffing. J Neuropsychopharmacology 30:1019–1027

    Article  Google Scholar 

  • Cam MC, Li WM, McNeill JH (1997) Partial preservation of pancreatic beta cells by vanadium: evidence for long-term amelioration of diabetes. Metabolism 46:769–778

    Article  CAS  PubMed  Google Scholar 

  • Cochrane AL, Kett MM, Samuel CS, Campanale NV, Anderson WP (2005). Renal structural and functional repair in a mouse model of reversal of ureteral obstruction. J Am Soc Nephrol 16: 3623–3630.

  • Corcoran GB, Fix L, Jones DP, Moslen MT, Nicotera P, Oberhaimmer FZ, Buttyan R (1994) Apoptosis: molecular control point in toxicity. Toxicol Appl Phamarcol J 128:169–181

    Article  CAS  Google Scholar 

  • Ebokaiwe AP, Adedara IA, Owoeye O, Farombi EO (2013) Neurotoxicity of Nigerian bonny light crude oil in rats. Drug Chem Toxicol 36:187–195

    Article  CAS  PubMed  Google Scholar 

  • Ercal N, Gurer-Orhan H, Aykin-Burns N (2001) Toxic metal and oxidative stress part 1: mechanism involved in metal induced oxidative damage. Curr Top Med Chem J 6:529–539

    Article  Google Scholar 

  • Farombi EO, Tahnteng JG, Agboola AO, Nwankwo JO, Emerole GO (2000) Chemoprevention of 2-acetylaminofluoreneinduced hepatotoxicity and lipid peroxidation in rats by kolaviron a Garcinia Kola seed extract. J Food Chem Toxicol 38:535–541

    Article  CAS  Google Scholar 

  • Farombi EO, Adedara IA, Ebokaiwe AP, Teberen R, Ehwerhemuepha T (2010) Nigerian bonny light crude oil disrupts antioxidant systems in the testes and sperm of rats. Arch Environ Contam Toxicol 59:166–174

    Article  CAS  PubMed  Google Scholar 

  • Flora SJS, Pachauri V (2010) Chelation in metal intoxication. Int J Environ Res Public Health 7(7):2745–2788

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Folarin O, Olopade F, Onwuka S, Olopade J (2016) Memory deficit recovery after chronic vanadium exposure in mice. J Oxidative Med Cell Longev (Spec Issue) 2016:7

    Google Scholar 

  • Fortoul TI, Rodriguez-Lara V, González-Villalva A et al (2014) Inhalation of vanadium pentoxide and its toxic effects in a mouse model. Inorg Chim Acta 420:8–15

    Article  CAS  Google Scholar 

  • Fukai T, Ushio-Fukai M (2011) Superoxide dismutases: role in redox signaling, vascular function, and diseases. Antioxid Redox Signal J 15:1583–1606

    Article  CAS  Google Scholar 

  • Garcia GB, Quiroga AD, Sturtz N, Martinez AL, Biancardi ME (2004) Morphological alterations of central nervous system (CNS) myelin in vanadium (V)-exposed adult rats. J Drug Chem Toxicol 27:281–293

    Article  CAS  Google Scholar 

  • Garcia GB, Biancardi ME, Quiroga AD (2005) Vanadium (V)-induced neurotoxicity in the rat central nervous system: a Histo-Immunohistochemical study. J Drug Chem Toxicol 28:329–344

    Article  CAS  Google Scholar 

  • Giergiel M, Kankofer M (2015) Age and sex-related changes in superoxide dismutase activity in bovine tissues. Czech J Anim Sci 60(8):367–374

    Article  Google Scholar 

  • Gligorovski S, Strekowski R, Barbati S, Vione D (2015) Environmental implications of hydroxyl radicals (•OH). Chem Rev 155:13051–13092

    Article  Google Scholar 

  • Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JK, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem J 126:131–136

    Article  CAS  Google Scholar 

  • Groot H, Littauer A (1989) Hypoxia, reactive oxygen and cell injury. Free Radic Biol Med J 6:541–551

    Article  Google Scholar 

  • Habig WH, Pabst MJ, Jacoby WB (1974) Glutathione-Stransferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  PubMed  Google Scholar 

  • Hosseini M, Seyedrazi N, Shahraki J, Pourahmad J (2012) Vanadium induces liver toxicity through reductive activation by glutathione and mitochondrial dysfunction. Adv Biosci Biotechnol 3:1096–1103

    Article  Google Scholar 

  • Hubatsch I, Ridderstrom M, Mannervik B (1998) Human glutathione transferase A4–4: An alpha class enzyme with high catalytic efficience in the conjugation of 4-hydroxynonenal and other genotoxic products of lipid peroxidation. Biochem J 15:175–179

    Article  Google Scholar 

  • Ibrahim D, Froberg B, Wolf A, Rusyniak DE (2006) Heavy metal poisoning: clinical presentations and pathophysiology. J Clin Lab Med 26:67–97

    Article  Google Scholar 

  • Igado OO, Olopade JO, Adesida A, Aina OO, Farombi EO (2012) Morphological and biochemical investigation into the possible neuroprotective effects of kolaviron (Garcinia kola bioflavonoid) on the brains of rats exposed to vanadium. Drug Chem Toxicol 35:370–380

    Article  Google Scholar 

  • Jollow DJ, Mitchell JR, Zamppaglione Z, Gillette JR (1974) Bromobenzene induced liver necrosis. Protective role of glutathione and evidence for 3, 4- bromobenzene oxide as the hepatotoxic metabolites. J Pharmacol 11:151–157

    Article  CAS  Google Scholar 

  • Kamal EM, Tamara S, Shaban D, Bauyrzhan U (2011) Combined effect of vanadium and nickel on lipidperoxidation and selected parameters of antioxidantsystem in liver and kidney of male rat. Afr J Biotechnology 10:18319-18325

  • Korbecki J, Baranowska-Bosiacka I, Gutowska I, Chlubek D (2015) Vanadium compounds as pro-inflammatory agents: effects on cyclooxygenases. Science 16:12648–12668

    CAS  Google Scholar 

  • Mahmoud KE, Shalahmetova T, Deraz S, Umbayev B (2011) Combined effect of vanadium and nickel on lipid peroxidation and selected parameters of antioxidant system in liver and kidney of male rat. Afr J Biotechnol 79:18319–18325

    Google Scholar 

  • Margret ES (2013) Chelation: harnessing and enhancing heavy metal detoxification. A Rev Sci World J 2013:219840

    Google Scholar 

  • Marouane W, Soussi A, Murat J, Bezzine S, Feki A (2011) The protective effect of Malva Sylvestris on rat kidney damaged by vanadium. Lipids Health Dis 10:65

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Melo A, Monteiro L, Lima LMF, de Oliveira DM, De Cerqueira MD, El-Bachá RS (2011) Oxidative stress in neurodegenerative diseases: mechanisms and therapeutic perspectives. J Oxidative Med Cell Longev 2011:14

    Google Scholar 

  • Meng Q, Wong YT, Chen J, Ruan R (2007) Age-related changes in mitochondrial function and antioxidative enzyme activity in Fischer 344 rats. Mech Ageing Dev 128:286–292

    Article  CAS  PubMed  Google Scholar 

  • Misra H, Fridovich I (1972) The role of superoxide anion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247(10):3170–3175

  • Monleau M, De M’,eo M, Paquet F, Chazel V, Dum’enil G, Donnadieu-Claraz M (2006) Genotoxic and inflammatory effects of depleted uranium particles inhaled by rats. J Toxicol Sci 89:287–295

  • Moskalyk RR, Alfantazi AM (2003) Processing of vanadium: a review. Miner Eng 16:793–805

    Article  CAS  Google Scholar 

  • Navarro A, Sanchez Del Pino MJ, Gomez C, Peralta JL, Boveris A (2002) Behavioral dysfunction, brain oxidative stress, and impaired mitochondrial electron transfer in ageing mice. Am J Physiol Regul Integr Comp Physiol 282:985–992

    Article  Google Scholar 

  • Ngwa HA, Kanthasamy A, Anantharam V, Witte CST, Houk R, Kanthasamy AG (2009) Vanadium induces dopaminergic neurotoxicity via protein kinase Cdelta dependent oxidative signaling mechanisms: relevance to etiopathogenesis of Parkinson's disease. J Toxicol Appl Pharmacol 240:273–285

    Article  Google Scholar 

  • Olopade JO, Connor JR (2010) Vanadium and neurotoxicity: a review. Curr Top Toxicol 7:33–39

    Google Scholar 

  • Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Biochemical role as a component of glutathione peroxidase. Sci J 179:588–590

    Article  CAS  Google Scholar 

  • Santos R, de Ruiz Almodóvar C, Bulteau A, Gomes CM (2013) Neurodegeneration, neurogenesis, and oxidative stress. Adv Biosci Biotechnol J 3:1096–1103

  • Sardar K, Ali S, Hameed S, Afzal S, Fatima S, Shakoor MB, Bharwana SA, Tauqeer HM (2013) Heavy metals contamination and what are the impacts on living organisms. Greener J Environ Manag Public Saf 2:172–179

    Article  Google Scholar 

  • Sasi MM, Haider SS, el-Fakhri M, Ghwarsha KM (1994) Microchromatographic analysis of lipids, protein, and occurrence of lipid peroxidation in various brain areas of vanadium exposed rats: a possible mechanism of vanadium neurotoxicity. Neurotoxicology 15:413–420

  • Ścibior A, Gołębiowska D, Niedźwiecka I (2013) Magnesium can protect against vanadium-induced lipid peroxidation in the hepatic tissue. J Oxidative Med Cell Longev. doi:10.1155/2013/802734

  • Sears ME (2013) Chelation: harnessing and enhancing heavy metal detoxification—a review. Sci World J 2013:13

    Article  Google Scholar 

  • Sobocanec S, Balog T, Sverko V (2003) Sex-dependent antioxidant enzyme activities and lipid peroxidation in ageing mouse brain. Free Radic Res 37:743–748

    Article  CAS  PubMed  Google Scholar 

  • Sohal RS, Arnold L, Orr WC (1990) Effect of age on superoxide dismutase, catalase, glutathione reductase, inorganic peroxides, TBA-reactive material, GSH/GSSG, NADPH/ NADP+ and NADH/NAD+ in Drosophila Melanogaster. Mech Ageing Dev 56:223–235

    Article  CAS  PubMed  Google Scholar 

  • Stohs SJ, Ragchi D (1995) Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 18(1999):321–336

    Article  CAS  PubMed  Google Scholar 

  • Todorich B, Olopade JO, Surguladze N, Zhang X, Neely E, Connor JR (2011) The mechanism of vanadium-mediated developmental Hypomyelination is related to destruction of oligodendrocyte progenitors through a relationship with ferritin and iron. Neurotox Res J 19:361–373

    Article  CAS  Google Scholar 

  • Uboh FE, Ufot SU, Eyong EU (2013) Comparative effect of withdrawal from exposure on gasoline and diesel induced nephrotoxicity in male albino Wistar rats. J Clin Toxicol 3:170

    Google Scholar 

  • Valko M, Rhodes CJ, Moncola J, Izakovic M, Mazur M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. J Chem Biol Interact 160:1–40

    Article  CAS  Google Scholar 

  • Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84

    Article  CAS  PubMed  Google Scholar 

  • Wolff SP (1994) Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods Enzymol J 233:182–189

    Article  CAS  Google Scholar 

  • Wozniak A, Drewa G, Wozniak B, Schachtschabel DO (2004) Activity of antioxidant enzymes and concentration of lipid peroxidation products in selected tissues of mice of different ages, both healthy and melanoma-bearing. Z Gerontol Geriatr 37:184–189

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

    O. R. Folarin & J. O. Olopade

  2. Department of Medical Laboratory Science, Ladoke Akintola University, Oshogbo, Nigeria

    O. R. Folarin

  3. Department of Biochemistry, Faculty of Basic Medical Sciences, University of Ibadan, Ibadan, Nigeria

    O. A. Adaramoye & O. O. Akanni

Authors
  1. O. R. Folarin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. A. Adaramoye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. O. Akanni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. O. Olopade
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. O. Olopade.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest regarding this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Folarin, O.R., Adaramoye, O.A., Akanni, O.O. et al. Changes in the brain antioxidant profile after chronic vanadium administration in mice. Metab Brain Dis 33, 377–385 (2018). https://doi.org/10.1007/s11011-017-0070-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11011-017-0070-9

Keywords

Search

Navigation