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Metabolic Brain Disease

, Volume 25, Issue 1, pp 11–15 | Cite as

Portacaval anastomosis-induced hyperammonemia does not lead to oxidative stress

  • Xiaoling Yang
  • Cristina R. Bosoi
  • Wenlei Jiang
  • Mélanie Tremblay
  • Christopher F. Rose
Original Paper

Abstract

Ammonia is neurotoxic and believed to play a major role in the pathogenesis of hepatic encephalopathy (HE). It has been demonstrated, in vitro and in vivo, that acute and high ammonia treatment induces oxidative stress. Reactive oxygen species (ROS) are highly reactive and can lead to oxidization of proteins resulting in protein damage. The present study was aimed to assess oxidative status of proteins in plasma and brain (frontal cortex) of rats with 4-week portacaval anastomosis (PCA). Markers of oxidative stress, 4-hydroxy-2-nonenal (HNE) and carbonylation were evaluated by immunoblotting in plasma and frontal cortex. Western blot analysis did not demonstrate a significant difference in either HNE-linked or carbonyl derivatives on proteins between PCA and sham-operated control rats in both plasma and frontal cortex. The present study suggests PCA-induced hyperammonemia does not lead to systemic or central oxidative stress.

Keywords

Hepatic encephalopathy PCA Oxidative stress Hyperammonemia 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Xiaoling Yang
    • 1
  • Cristina R. Bosoi
    • 1
  • Wenlei Jiang
    • 1
  • Mélanie Tremblay
    • 1
  • Christopher F. Rose
    • 1
  1. 1.Neuroscience Research UnitUniversité de Montréal (CRCHUM)MontrealCanada

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