LncRNA EWSAT1 upregulates CPEB4 via miR-330-5p to promote cervical cancer development

Abstract

Long non‐coding RNA (lncRNA) Ewing sarcoma associated transcript 1 (EWSAT1) is an oncogene in a variety of tumors. Here, we planned to demonstrate EWSAT1 function in cervical cancer and further illustrate its underlying mechanism. EWSAT1 expression in cervical cancer was evaluated through qRT-PCR. Colony forming capacity was measured by colony formation assay and cell proliferation ability was measured by CCK-8 kit. Wound healing experiment was applied to test the cell migration and transwell assay was applied to test the invasion ability. Luciferase assay was employed to demonstrate EWSAT1 and miR-330-5p interaction. In cervical cancer, the expression of EWSAT1 was enhanced and contributed to the poor prognosis. Downregulated EWSAT1 expression inhibited Hela cell migration, proliferation, and invasion. EWSAT1 targeted to miR-330-5p and upregulated cytoplasmic polyadenylation element-binding protein 4 (CPEB4) expression by sponging miR-330-5p. Our study revealed that EWSAT1 enhances CPEB4 expression through sponging miR-330-5p, thereby promoting cervical cancer development, which might provide potential therapeutic targets for clinically cervical cancer patients.

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Correspondence to Qingyan Zhou.

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Zhou, Q., Xie, Y., Wang, L. et al. LncRNA EWSAT1 upregulates CPEB4 via miR-330-5p to promote cervical cancer development. Mol Cell Biochem 471, 177–188 (2020). https://doi.org/10.1007/s11010-020-03778-8

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Keywords

  • Long non‐coding RNA (lncRNA) ewing sarcoma associated transcript 1 (EWSAT1)
  • miR-330-5p
  • Cytoplasmic polyadenylation element-binding protein 4 (CPEB4)
  • Cervical cancer