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WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway

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Abstract

Skeletal muscle is one of the most important tissues of the human body necessary for sporting activities. The differentiation of muscle-derived satellite cells (MDSCs) plays an important role in the development and regeneration of skeletal muscles. Similarly, the Wnt/β-catenin signalling pathway plays an important role in the process of muscle differentiation. Wnt1-inducible signalling pathway protein-1 (WISP1), a downstream protein of the Wnt/β-catenin signalling pathway and a member of the CCN family that also plays an important role in the differentiation process, and its expression increase during the differentiation of bovine MDSCs. However, its role in MDSC differentiation is poorly understood. Therefore, we investigated the mechanisms regulating this process via Western blot and immunofluorescence staining. Immunoprecipitation and mass spectrometry detected annexin A1 (ANXA1), a protein that interacts with WISP1. To determine whether WISP1 influences TGF-β signalling and differentiation independently of ANXA1, the latter was knocked down, while WISP1 was activated. WISP1 expression increased significantly during bovine MDSC differentiation. However, WISP1 did not affect the TGF-β signalling pathway protein marker when ANXA1 was inhibited. Taken together, WISP1 regulates the TGF-β signalling pathway through ANXA1 recruitment, thereby promoting bovine MDSC differentiation, suggesting the Wnt/β-catenin signalling pathway as another target to promote cell differentiation.

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All data generated or analysed during this study are included in this published article and its supplementary information files.

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Funding

This work was supported by the Natural Science Foundation of Heilongjiang Province (Grant No. C2017025).

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Authors and Affiliations

  1. The Laboratory of Cell and Development, Northeast Agricultural University, Changjiang Road No. 600, Xiang Fang District, Harbin, 150030, Heilongjiang, China

    Chunyu Zhang, Yuhan Zhang, Wenyu Zhang, Huili Tong, Shufeng Li & Yunqin Yan

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  1. Chunyu Zhang
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  2. Yuhan Zhang
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  3. Wenyu Zhang
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Contributions

CZ, HT, SL, and YY involved in conceptualization; CZ and YZ took part in methodology; CZ and WZ performed formal analysis and investigation; CZ involved in writing—original draft preparation; CZ and YY performed writing—review and editing; and HT and SL participated in supervision.

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Correspondence to Yunqin Yan.

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Zhang, C., Zhang, Y., Zhang, W. et al. WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway. Mol Cell Biochem 470, 215–227 (2020). https://doi.org/10.1007/s11010-020-03763-1

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