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Acetyl-11-keto-β-boswellic acid modulates membrane dynamics in benzo(a)pyrene-induced lung carcinogenesis

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Abstract

Membrane fluidity is the most important physiochemical property of cell membranes and governs its functional attributes. The current investigations were undertaken to understand the potential role of acetyl-11-keto-β-boswellic acid (AKBA), if any, on regulation of membrane dynamics under conditions of benzo(a)pyrene (BaP)-induced lung carcinogenesis in female rats. The animals were divided into five groups which included (I) Normal control, (II) Vehicle treated (olive oil), (III) BaP treated, (IV) AKBA treated and (V) BaP + AKBA treated. BaP was administered at a dose level of 50 mg/kg b.wt. in olive oil orally twice a week for 4 weeks. AKBA was given at a dose level of 50 mg/kg b.wt. in olive oil orally thrice a week for 24 weeks. In addition, AKBA was also administered at a similar dose to BaP-treated animals 4 weeks prior to BaP administration and continued for another 20 weeks. The lipid profile and membrane dynamics were analysed in lung tissue. Total lipids, phospholipids content, membrane fluidity, polarization and order of membrane were significantly (p ≤ 0.001) increased in BaP-exposed animals. However, significant decrease was observed in glycolipids, cholesterol, microviscosity and anisotropy levels compared with normal control animals. Appreciable improvements in above indices were recorded when AKBA was administered to BaP-treated animals. Moreover, the structural variations observed in Fourier-transform infrared spectroscopy spectrum were also normalized in BaP-treated rats with AKBA supplementation. This suggests that the AKBA has a potential role in improving membrane fluidity and associated lipid content in BaP-induced lung carcinogenesis.

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Acknowledgement

The authors are thankful to the Department of Science and Technology, India for providing financial assistance in the form of DST-Inspire Fellowship (IF160041) to Priti Bhardwaj. The authors are also thankful to the University Grants Commission, New Delhi, India for providing financial assistance in terms of Dr. D.S. Kothari Postdoctoral Fellowship (DSKPDF) award to the co-authors (Dr. Manoj Kumar and Dr. Sunil Kumar Dhatwalia). The authors also acknowledge the financial assistance under DST-PURSE grant to the Department of Biophysics, Panjab University, Chandigarh, India.

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  1. Department of Biophysics, Panjab University, Chandigarh, India

    Priti Bhardwaj, Manoj Kumar, Sunil Kumar Dhatwalia, Mohan Lal Garg & Devinder Kumar Dhawan

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  1. Priti Bhardwaj
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Bhardwaj, P., Kumar, M., Dhatwalia, S.K. et al. Acetyl-11-keto-β-boswellic acid modulates membrane dynamics in benzo(a)pyrene-induced lung carcinogenesis. Mol Cell Biochem 460, 17–27 (2019). https://doi.org/10.1007/s11010-019-03566-z

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